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PGBD3 (NM_170753) Human Tagged ORF Clone

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SKU
RC208977
PGBD3 (Myc-DDK-tagged)-Human piggyBac transposable element derived 3 (PGBD3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $552.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PGBD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208977 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCGAACACTAAGTTTACATGAAATAACTGACCTTTTAGAGACAGATGACAGCATAGAAGCAAGTG
CTATAGTGATACAACCACCTGAAAATGCTACAGCACCTGTTTCTGATGAGGAATCAGGAGATGAAGAAGG
TGGAACAATAAATAATCTGCCAGGTTCTTTGTTGCACACAGCTGCGTATCTTATTCAAGATGGCTCTGAT
GCTGAGTCTGACTCAGATGATCCCTCATACGCACCTAAAGATGACTCTCCTGATGAAGTTCCATCTACGT
TTACTGTGCAGCAACCTCCACCATCAAGGAGGAGGAAAATGACAAAAATTCTTTGCAAATGGAAAAAAGC
CGACCTAACTGTACAACCCGTAGCAGGTAGAGTTACAGCACCACCAAACGATTTCTTCACCGTAATGAGA
ACTCCCACAGAAATTCTTGAACTTTTTCTTGATGACGAGGTCATTGAACTCATTGTCAAGTACTCCAACT
TATATGCTTGCAGTAAAGGTGTACATCTTGGCTTGACTAGCTCTGAATTCAAATGTTTTCTGGGAATTAT
TTTTCTGAGTGGTTATGTCTCAGTTCCTAGAAGGCGTATGTTTTGGGAACAAAGAACAGATGTGCATAAT
GTACTGGTTAGTGCTGCCATGAGACGTGACCGGTTTGAAACTATATTTTCTAATTTGCATGTTGCTGACA
ATGCAAATTTGGATCCAGTGGACAAATTTTCCAAATTGCGACCTCTCATAAGCAAACTTAATGAGAGATG
CATGAAATTTGTTCCAAATGAAACATATTTCAGCTTTGATGAATTCATGGTTCCTTATTTTGGTCGTCAC
GGGTGCAAACAATTTATTCGGGGAAAGCCCATTCGGTTTGGCTATAAGTTTTGGTGTGGTGCCACCTGTC
TGGGCTACATTTGCTGGTTTCAGCCGTATCAGGGTAAAAACCCAAATACTAAACATGAGGAATATGGTGT
CGGTGCGTCACTTGTCCTTCAGTTTAGTGAGGCACTTACAGAGGCACACCCTGGACAATACCATTTTGTA
TTCAATAACTTTTTCACCAGTATTGCACTTCTTGATAAGCTCAGTTCAATGGGACATCAGGCAACAGGTA
CAGTGAGAAAGGATCACATTGACAAAGTTCCACTGGAATCAGATGTAGCTTTAAAGAAAAAAGAAAGAGG
CACATTTGATTATCGAATTGATGGCAAAGGCAATATTGTCTGCAGATGGAATGATAACAGTGTTGTCACT
GTTGCCTCATCTGGTGCTGGTATCCATCCCCTGTGTCTTGTCAGTCGTTACTCCCAGAAACTGAAAAAGA
AGATACAAGTTCAGCAGCCAAACATGATCAAAGTGTATAACCAGTTCATGGGAGGCGTAGACAGAGCTGA
TGAAAACATTGATAAGTATCGGGCATCAATCCGTGGAAAGAAATGGTATTCAAGCCCTCTTTTGTTCTGT
TTCGAATTGGTCTTACAAAATGCTTGGCAATTGCATAAAACATATGATGAGAAACCAGTGGATTTTCTGG
AGTTTCGTCGACGTGTGGTATGCCATTATCTGGAGACCCATGGTCATCCTCCAGAACCTGGCCAAAAAGG
AAGACCTCAGAAGCGTAACATTGACTCACGTTATGATGGCATAAATCATGTGATAGTCAAACAGGGAAAG
CAAACGCGATGCGCTGAATGTCATAAGAACACAACTTTTCGATGTGAAAAATGTGATGTTGCCTTACATG
TGAAGTGTTCCGTTGAATATCACACTGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208977 protein sequence
Red=Cloning site Green=Tags(s)

MPRTLSLHEITDLLETDDSIEASAIVIQPPENATAPVSDEESGDEEGGTINNLPGSLLHTAAYLIQDGSD
AESDSDDPSYAPKDDSPDEVPSTFTVQQPPPSRRRKMTKILCKWKKADLTVQPVAGRVTAPPNDFFTVMR
TPTEILELFLDDEVIELIVKYSNLYACSKGVHLGLTSSEFKCFLGIIFLSGYVSVPRRRMFWEQRTDVHN
VLVSAAMRRDRFETIFSNLHVADNANLDPVDKFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRH
GCKQFIRGKPIRFGYKFWCGATCLGYICWFQPYQGKNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFV
FNNFFTSIALLDKLSSMGHQATGTVRKDHIDKVPLESDVALKKKERGTFDYRIDGKGNIVCRWNDNSVVT
VASSGAGIHPLCLVSRYSQKLKKKIQVQQPNMIKVYNQFMGGVDRADENIDKYRASIRGKKWYSSPLLFC
FELVLQNAWQLHKTYDEKPVDFLEFRRRVVCHYLETHGHPPEPGQKGRPQKRNIDSRYDGINHVIVKQGK
QTRCAECHKNTTFRCEKCDVALHVKCSVEYHTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_170753
ORF Size 1779 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_170753.3
RefSeq Size 2266 bp
RefSeq ORF 1782 bp
Locus ID 267004
UniProt ID Q8N328
Cytogenetics 10q11.23
MW 67.6 kDa
Summary This gene is a member of a small family of genes derived from piggyBac transposable elements. The encoded protein contains a zinc-ribbon domain characteristic of transposon-derived proteins and may function as a regulator of transcription. Alternative splicing occurs between a splice site from exon 5 of the adjacent upstream gene 'excision repair cross-complementation group 6' (ERCC6, GeneID: 2074) and the 3' splice site upstream of the open reading frame (ORF) of this gene, which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. Pseudogenes for this gene are defined on chromosomes 4, 5 and 12. provided by RefSeq, Mar 2016
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"NM_170753" in other vectors (4)
SKU Description Size Price
RC208977L3 Lenti ORF clone of Human piggyBac transposable element derived 3 (PGBD3), Myc-DDK-tagged 10 ug
$852.00
RC208977L4 Lenti ORF clone of Human piggyBac transposable element derived 3 (PGBD3), mGFP tagged 10 ug
$852.00
RG208977 PGBD3 (tGFP-tagged) - Human piggyBac transposable element derived 3 (PGBD3) 10 ug
$489.00 $752.00
SC100698 PGBD3 (untagged)-Human piggyBac transposable element derived 3 (PGBD3) 10 ug
$553.00

Plasmid Map for RC208977

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208977
Circular map for RC208977

file-alt Citations

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