PRRT1 (NM_030651) Human Tagged ORF Clone

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SKU
RC208958
PRRT1 (Myc-DDK-tagged)-Human proline-rich transmembrane protein 1 (PRRT1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRRT1
Synonyms C6orf31; DSPD1; IFITMD7; NG5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208958 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCATCCGAAAAGTCAGGACTCCCAGACTCAGTCCCTCACACTTCTCCGCCGCCCTACAATGCCCCTC
AGCCTCCAGCCGAACCCCCAGCCCCACCGCCACAGGCAGCCCCTTCCTCACACCATCACCACCACCACCA
CTACCATCAGTCTGGCACCGCCACCCTCCCGCGCTTAGGGGCAGGGGGCCTGGCCTCTTCCGCGGCCACC
GCTCAGCGCGGTCCCTCCTCCTCTGCCACGCTGCCGAGGCCCCCCCACCACGCCCCTCCCGGCCCTGCTG
CCGGGGCACCCCCACCCGGCTGCGCTACCTTGCCCCGCATGCCACCCGACCCTTACCTGCAGGAGACTCG
CTTCGAGGGCCCACTTCCCCCGCCGCCGCCCGCTGCCGCCGCCCCGCCCCCGCCGGCGCCAGCCCAGACT
GCCCAGGCCCCTGGCTTCGTGGTGCCCACGCACGCGGGGACTGTGGGCACGCTGCCGCTGGGGGGCTACG
TAGCGCCCGGATACCCCCTGCAGCTGCAGCCTTGCACTGCTTACGTGCCGGTCTACCCGGTGGGCACGCC
ATATGCAGGCGGGACCCCGGGGGGAACAGGAGTGACCTCCACTCTCCCCCCGCCGCCCCAGGGCCCAGGG
CTGGCCCTACTGGAGCCGAGGCGCCCGCCACACGACTACATGCCCATCGCGGTGCTGACCACCATCTGTT
GCTTCTGGCCTACTGGCATCATTGCCATCTTCAAGGCCGTGCAGGTGCGCACGGCCTTGGCCCGCGGAGA
CATGGTGTCGGCCGAGATCGCTTCACGCGAGGCCCGGAACTTCTCCTTCATCTCCCTGGCCGTGGGCATC
GCGGCCATGGTGCTCTGTACCATCCTCACCGTAGTCATCATCATCGCCGCGCAGCACCACGAGAACTACT
GGGATCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208958 protein sequence
Red=Cloning site Green=Tags(s)

MSSEKSGLPDSVPHTSPPPYNAPQPPAEPPAPPPQAAPSSHHHHHHHYHQSGTATLPRLGAGGLASSAAT
AQRGPSSSATLPRPPHHAPPGPAAGAPPPGCATLPRMPPDPYLQETRFEGPLPPPPPAAAAPPPPAPAQT
AQAPGFVVPTHAGTVGTLPLGGYVAPGYPLQLQPCTAYVPVYPVGTPYAGGTPGGTGVTSTLPPPPQGPG
LALLEPRRPPHDYMPIAVLTTICCFWPTGIIAIFKAVQVRTALARGDMVSAEIASREARNFSFISLAVGI
AAMVLCTILTVVIIIAAQHHENYWDP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030651
ORF Size 918 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030651.4
RefSeq Size 1931 bp
RefSeq ORF 921 bp
Locus ID 80863
UniProt ID Q99946, A0A1U9X8D6
Cytogenetics 6p21.32
Protein Families Transmembrane
MW 31.4 kDa
Summary Required to maintain a pool of extrasynaptic AMPA-regulated glutamate receptors (AMPAR) which is necessary for synapse development and function. Regulates basal AMPAR function and synaptic transmission during development but is dispensable at mature hippocampal synapses. Plays a role in regulating basal phosphorylation levels of glutamate receptor GRIA1 and promotes GRIA1 and GRIA2 cell surface expression.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030651" in other vectors (4)
SKU Description Size Price
RC208958L3 Lenti-ORF clone of PRRT1 (Myc-DDK-tagged)-Human proline-rich transmembrane protein 1 (PRRT1) 10 ug
$600.00
RC208958L4 Lenti-ORF clone of PRRT1 (mGFP-tagged)-Human proline-rich transmembrane protein 1 (PRRT1) 10 ug
$600.00
RG208958 PRRT1 (tGFP-tagged) - Human proline-rich transmembrane protein 1 (PRRT1) 10 ug
$489.00 $500.00
SC122989 PRRT1 (untagged)-Human proline-rich transmembrane protein 1 (PRRT1) 10 ug
$300.00

Plasmid Map for RC208958

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Cloning scheme for RC208958

Circular map for RC208958

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