SOX9 (NM_000346) Human Tagged ORF Clone
$711.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SOX9 |
| Synonyms | CMD1; CMPD1; SRA1; SRXX2; SRXY10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000346 |
| ORF Size | 1527 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000346.4 |
| RefSeq Size | 3935 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 6662 |
| UniProt ID | P48436 |
| Cytogenetics | 17q24.3 |
| Domains | HMG |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| MW | 56 kDa |
| Summary | The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000346" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208944L1 | Lenti-ORF clone of SOX9 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) | 10 ug |
$1,011.00
|
|
| RC208944L2 | Lenti-ORF clone of SOX9 (mGFP-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) | 10 ug |
$1,011.00
|
|
| RC208944L3 | Lenti-ORF clone of SOX9 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) | 10 ug |
$1,011.00
|
|
| RC208944L4 | Lenti-ORF clone of SOX9 (mGFP-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) | 10 ug |
$1,011.00
|
|
| RG208944 | SOX9 (tGFP-tagged) - Human SRY (sex determining region Y)-box 9 (SOX9) | 10 ug |
$911.00
|
|
| SC321884 | SOX9 (untagged)-Human SRY (sex determining region Y)-box 9 (SOX9) | 10 ug |
$475.00
|
|
Citations
Repression of Wnt/β-catenin signaling by SOX9 and Mastermind-like transcriptional coactivator 2
Sinha, Fan, Ramakrishnan et al
Sci Adv (2021) 7 (8) DOI: 10.1126/sciadv.abe0849
Sinha, Fan, Ramakrishnan et al
Sci Adv (2021) 7 (8) DOI: 10.1126/sciadv.abe0849
Cited by 41
AI Summary: OriGene’s human SOX9 open reading frame (ORF; NM_000346) was used to generate SOX9 expression constructs that enabled the study of SOX9’s role in repressing Wnt/β-catenin signaling in this paper.
Snippet: Biolabs, and all the gBlocks and primers (listed in table S1) were ordered from Integrated DNA Technologies. The human SOX9 open reading frame (ORF) was cloned via PCR amplification of the SOX9 ORF (OriGene, NM_000346) and inserted in frame into the Bam HI/Xho I sites of 2×Flag pcDNA3.1. SOX9 ORF (OriGene;NM_000346) was cloned into Bam HI/Xho I sites, and all the mutated versions of SOX9 (Fig
A retrotransposon gag-like-3 gene RTL3 and SOX-9 co-regulate the expression of COL2A1 in chondrocytes
Ball, Ansari, Ahmad et al
Connect Tissue Res (2020)
Ball, Ansari, Ahmad et al
Connect Tissue Res (2020)
Cited by 6
SOX9 promotes stress-responsive transcription of VGF nerve growth factor inducible gene in renal tubular epithelial cells
Kim, Bai, Jayne et al
J. Biol. Chem. (2020) 295 (48), 16328-16341 DOI: 10.1074/jbc.RA120.015110
Kim, Bai, Jayne et al
J. Biol. Chem. (2020) 295 (48), 16328-16341 DOI: 10.1074/jbc.RA120.015110
AI Summary: OriGene’s Sox9 expression vector (pCMV6-Sox9) was used to generate stably transfected HEK293 cells for promoter luciferase assays, enabling the study of Sox9’s role in regulating VGF gene transcription in renal tubular epithelial cells.
Snippet: Promoter Luciferase Assay. HEK293 cells were stably transfected with either empty vector (pCMV6) or Sox9 expression vector (Origene). …
SOX9 promotes stress-responsive transcription of VGF nerve growth factor inducible gene in kidney epithelial cells
Kim, Bai, Jayne et al
bioRxiv (2020)
Kim, Bai, Jayne et al
bioRxiv (2020)
Cited by 1
Snippet: 48 hours. Promoter Luciferase Assay. HEK293 cells were stably transfected with either empty vector (pCMV6) or Sox9 expression vector (Origene). These cells were then utilized for promoter luciferase reporter assays using methods reported in our recent studies (31
Changes in Fluorescence Recovery After Photobleaching (FRAP) as an indicator of SOX9 transcription factor activity
Govindaraj, Hendriks, Lidke et al
Biochim Biophys Acta Gene Regul Mech (2018)
Govindaraj, Hendriks, Lidke et al
Biochim Biophys Acta Gene Regul Mech (2018)
AI Summary: OriGene products SOX9 cDNA (RC208944) and mGFP (PS100040) were used to construct SOX9-mGFP fusion proteins, enabling the study of SOX9 transcription factor activity via FRAP in this paper.
Snippet: ;The vector expressing SOX9-mGFP was constructed by cloning mGFP (PS100040, Origene) with the C-terminal of wild type SOX9 (RC208944, Origene) using SgfI and MluI restriction sites. … Non-dimerizing and non-DNA binding mutated SOX9-mGFP, A76E and W143R respectively, were generated from SOX9-mGFP by site-directed mutagenesis (210518, Agilent). …
Odd-skipped related 1 inhibits lung cancer proliferation and invasion by reducing Wnt signaling through the suppression of SOX9 and β-catenin
Wang, Lei, Zheng et al
Cancer Sci. (2018) 109 (6), 1799-1810 DOI: 10.1111/cas.13614
Wang, Lei, Zheng et al
Cancer Sci. (2018) 109 (6), 1799-1810 DOI: 10.1111/cas.13614
AI Summary: OriGene contributed pCMV6-OSR1, pCMV6-SOX9, pCMV6-CTNNB1, and pCMV6 control plasmids, which were used for gene overexpression experiments to investigate the roles of OSR1, SOX9, and β-catenin in lung cancer cell proliferation and invasion.
Snippet: … The pCMV6‐OSR1 plasmid, pCMV6‐SOX9 plasmid, pCMV6‐CTNNB1 plasmid and the control empty vector pCMV6 were purchased from Origene (Rockville, MD, USA). Small interfering (si)RNA against OSR1 (OSR1‐siRNA), SOX9‐siRNA, CTNNB1‐siRNA and control siRNA were synthesized by RIBOBIO (Guangzhou, China). …
Showing 1-6 of 11 papers.
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