CTR9 (NM_014633) Human Tagged ORF Clone
SKU
RC208933
CTR9 (Myc-DDK-tagged)-Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9)
$1,021.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CTR9 |
| Synonyms | p150; p150TSP; SH2BP1; TSBP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014633 |
| ORF Size | 3519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014633.3 |
| RefSeq Size | 4348 bp |
| RefSeq ORF | 3522 bp |
| Locus ID | 9646 |
| UniProt ID | Q6PD62 |
| Cytogenetics | 11p15.4 |
| Domains | TPR |
| Protein Families | Protease |
| MW | 133.3 kDa |
| Summary | The protein encoded by this gene is a component of the PAF1 complex, which associates with RNA polymerase II and functions in transcriptional regulation and elongation. This complex also plays a role in the modification of histones. provided by RefSeq, Oct 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014633" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208933L1 | Lenti ORF clone of Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9), Myc-DDK-tagged | 10 ug |
$1,321.00
|
|
| RC208933L2 | Lenti ORF clone of Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9), mGFP tagged | 10 ug |
$1,321.00
|
|
| RC208933L3 | Lenti ORF clone of Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9), Myc-DDK-tagged | 10 ug |
$1,321.00
|
|
| RC208933L4 | Lenti ORF clone of Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9), mGFP tagged | 10 ug |
$1,321.00
|
|
| RG208933 | CTR9 (tGFP-tagged) - Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9) | 10 ug |
$789.00
$1,221.00
|
|
| SC114923 | CTR9 (untagged)-Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9) | 10 ug |
$1,022.00
|
|
| SC322682 | CTR9 (untagged)-Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9) | 10 ug |
$1,022.00
|
|
Citations
PAF1 complex interactions with SETDB1 mediate promoter H3K9 methylation and transcriptional repression of Hoxa9 and Meis1 in acute myeloid leukemia
Ropa, Saha, Chen et al
Oncotarget (2018) 9 (31), 22123-22136 DOI: 10.18632/oncotarget.25204
Ropa, Saha, Chen et al
Oncotarget (2018) 9 (31), 22123-22136 DOI: 10.18632/oncotarget.25204
Snippet: erated using site-directed mutagenesis using the QuikChangeXL kit according to the manufacturer’s protocol (Agilent). CMV-MYC-FLAG-PAF1, CMV-MYC-FLAG-CTR9, and CMV-MYC-FLAG-LEO1 were purchases from Origene. Cell line generation and cell culture conditions Cell lines were generated from either wild type C57Bl/6 (Taconic Farms) mouse bone marrow, Cdc73fl/fl-CreERT2[51] mouse bone marrow, or M1 mu
Drosophila CG2469 Encodes a Homolog of Human CTR9 and Is Essential for Development
Chaturvedi, Inaba, Scoggin et al
G3 (Bethesda) (2016) 6 (12), 3849-3857 DOI: 10.1534/g3.116.035196
Chaturvedi, Inaba, Scoggin et al
G3 (Bethesda) (2016) 6 (12), 3849-3857 DOI: 10.1534/g3.116.035196
AI Summary: OriGene's human CTR9 cDNA construct (NM_014633) was used in this study to rescue the Drosophila Ctr9 null phenotype, demonstrating functional conservation between species.
Snippet: ...cDNA construct NM_014633 (Origene) (see Supplemental Material...recombination (Rainbow Transgenics). Antibodies The following antibodies were used in this study: Rabbit...fluorescence-conjugated secondary antibodies were used at a 1:200 dilution...
Showing 1-2 of 2 papers.
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