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NRARP (NM_001004354) Human Tagged ORF Clone

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SKU
RC208806
NRARP (Myc-DDK-tagged)-Human NOTCH-regulated ankyrin repeat protein (NRARP)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NRARP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208806 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCAGGCCGAGCTGTCCACCTGCTCCGCGCCGCAGACGCAGCGCATCTTCCAGGAGGCTGTGCGCA
AGGGCAACACGCAGGAGCTGCAGTCGCTGCTGCAGAACATGACCAACTGCGAGTTCAACGTGAACTCGTT
CGGGCCCGAGGGCCAGACGGCGCTGCACCAGTCGGTCATCGACGGCAACCTGGAGCTCGTGAAGCTGCTG
GTCAAGTTCGGCGCCGACATCCGCCTGGCCAACCGCGACGGCTGGAGCGCGCTGCACATCGCCGCGTTCG
GTGGCCACCAGGACATCGTGCTCTATCTCATCACCAAGGCGAAGTACGCGGCCAGCGGCCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208806 protein sequence
Red=Cloning site Green=Tags(s)

MSQAELSTCSAPQTQRIFQEAVRKGNTQELQSLLQNMTNCEFNVNSFGPEGQTALHQSVIDGNLELVKLL
VKFGADIRLANRDGWSALHIAAFGGHQDIVLYLITKAKYAASGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004354
ORF Size 342 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004354.3
RefSeq Size 2639 bp
RefSeq ORF 345 bp
Locus ID 441478
UniProt ID Q7Z6K4
Cytogenetics 9q34.3
MW 12.5 kDa
Summary Downstream effector of Notch signaling. Involved in the regulation of liver cancer cells self-renewal (PubMed:25985737). Involved in angiogenesis acting downstream of Notch at branch points to regulate vascular density. Proposed to integrate endothelial Notch and Wnt signaling to control stalk cell proliferation and to stablilize new endothelial connections during angiogenesis (PubMed:19154719). During somitogenesis involved in maintenance of proper somite segmentation and proper numbers of somites and vertebrae. Required for proper anterior-posterior somite patterning. Proposed to function in a negative feedback loop to destabilize Notch 1 intracellular domain (NICD) and downregulate the Notch signal, preventing expansion of the Notch signal into the anterior somite domain (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001004354" in other vectors (4)
SKU Description Size Price
RC208806L3 Lenti ORF clone of Human NOTCH-regulated ankyrin repeat protein (NRARP), Myc-DDK-tagged 10 ug
$450.00
RC208806L4 Lenti ORF clone of Human NOTCH-regulated ankyrin repeat protein (NRARP), mGFP tagged 10 ug
$450.00
RG208806 NRARP (tGFP-tagged) - Human NOTCH-regulated ankyrin repeat protein (NRARP) 10 ug
$489.00
SC125684 NRARP (untagged)-Human NOTCH-regulated ankyrin repeat protein (NRARP) 10 ug
$150.00

Plasmid Map for RC208806

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208806
Circular map for RC208806

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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