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PRR14 (NM_024031) Human Tagged ORF Clone

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SKU
RC208696
PRR14 (Myc-DDK-tagged)-Human proline rich 14 (PRR14)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $545.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRR14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208696 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTTGCCCGGGGACTCCAGCCCGCCTGGCCAGCCGCGTCTGTGCCGCCAGCCTCTGACTCGAGCAT
TATGGGGAGCCAGGAGCCCGAAACGGCCGAGGCTGCAGCTCCCGGGGGCCCCTTCTCCCCTGGAAAAGGC
CTCTCGGCGGGTCCTGGCCGTGGTGCTAGAAGATGTCATGGCTGTTCACATGGTCCCCGTGGTGCCCTCA
AAGCAGACCTCCATACCACAGCACCACAGCTACCATCAGGATCCTGTCCACAGGCAGCCGCCTGCCTCGC
CACCCCGGCAGGCCGGGTGGTCCTCGCAGGCCAGGCCTCCCGACCCTCTGTGTTTGTGTCGCGAGCCCTT
GAGCCGCATCCACCGGACCTCTTCCACCCTGAGGCGGCGATCAAGGACAACCCCTGGCCCAGAGGAGGGC
CCTTCACAAAAGGTGGACCGGGCCCCCCAGCCCACCCTGGTGGTGATGCTGGAAGACATCGCCAGTCCTA
GACCCCCCGCTGAGGGCTTCATTGATGAGACCCCCAACTTCATCATCCCAGCACAAAGAGCTGAGCCCAT
GAGGATAGTTCGCCAGCCAACGCCTCCACCTGGGGACCTAGAACCCCCATTCCAGCCATCTGCTCTGCCT
GCAGACCCTCTGGAGAGCCCACCAACAGCCCCAGATCCTGCTCTGGAGCTCCCATCCACCCCACCACCGT
CCAGCCTTTTACGCCCCCGCCTCAGTCCCTGGGGCTTGGCCCCGCTCTTCCGTTCCGTCCGCTCCAAGCT
GGAGAGCTTTGCTGACATCTTCCTCACGCCCAACAAAACCCCACAGCCCCCACCCCCGTCCCCCCCAATG
AAGCTGGAGTTGAAGATCGCCATCTCAGAGGCCGAGCAGTCTGGGGCTGCTGAGGGCACTGCGTCTGTCA
GCCCCCGGCCCCCAATCCGCCAGTGGCGAACTCAGGACCACAATACCCCAGCACTTCTCCCTAAGCCCTC
TCTGGGCCGAAGCTACTCCTGCCCTGATCTGGGGCCCCCTGGCCCAGGTACCTGCACCTGGCCACCTGCT
CCACCCCAACCAAGCCGACCACGGCCGCGGCGGCACACTGTGGGTGGTGGGGAAATGGCCCGAGCCCCGC
CACCCCCTCGGCCCTGTCTCCGGAAAGAGGTCTTCCCTCTCGGAGGAGTGGGAGCCTCCCCTTCTCTCAC
CACATCTTGCTCGTCCACGGCATCCACTTCCTTCTCCGAACCAGCAGAACCCAGGTTGGGTTCAACCAAA
GGGAAGGAGCCAAGAGCCTCAAAGGACCAGGTGCTTTCAGAACCTGAGACCAAGACCATGGGAAAGGTTT
CTCGATTCAGAATACGCAGAACACCAGCCCGTCCTCAGCTAAACCTTACACCAATGGGACTGCCTCGACC
AATCAGGTTGAACAAGAAGGAGTTCAGCTTGGAAGAAATTTACACCAACAAGAATTACCAATCACCCACA
ACCAGGAGGACCTTTGAGACCATCTTTGAGGAACCCCGGGAGCGCAATGGGACTCTGATTTTCACCAGCT
CAAGGAAGCTCCGGCGGGCTGTGGAATTTCGGGACAGCAGCCTTCCTCGATCACGAAGACCGTCCCGTGG
GGTCCGGGCTGCAGGGGGCAGGACTGTTCCTCCCAATGTGGCCCCCAGCCCTGATGTGGGCCCCCTGCTC
CAGCAGCGGCTGGAGGAGCTAGATGCCTTGCTCCTGGAGGAAGAAACAGTAGATCGGGAGCAGCCCCACT
GGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208696 protein sequence
Red=Cloning site Green=Tags(s)

MDLPGDSSPPGQPRLCRQPLTRALWGARSPKRPRLQLPGAPSPLEKASRRVLAVVLEDVMAVHMVPVVPS
KQTSIPQHHSYHQDPVHRQPPASPPRQAGWSSQARPPDPLCLCREPLSRIHRTSSTLRRRSRTTPGPEEG
PSQKVDRAPQPTLVVMLEDIASPRPPAEGFIDETPNFIIPAQRAEPMRIVRQPTPPPGDLEPPFQPSALP
ADPLESPPTAPDPALELPSTPPPSSLLRPRLSPWGLAPLFRSVRSKLESFADIFLTPNKTPQPPPPSPPM
KLELKIAISEAEQSGAAEGTASVSPRPPIRQWRTQDHNTPALLPKPSLGRSYSCPDLGPPGPGTCTWPPA
PPQPSRPRPRRHTVGGGEMARAPPPPRPCLRKEVFPLGGVGASPSLTTSCSSTASTSFSEPAEPRLGSTK
GKEPRASKDQVLSEPETKTMGKVSRFRIRRTPARPQLNLTPMGLPRPIRLNKKEFSLEEIYTNKNYQSPT
TRRTFETIFEEPRERNGTLIFTSSRKLRRAVEFRDSSLPRSRRPSRGVRAAGGRTVPPNVAPSPDVGPLL
QQRLEELDALLLEEETVDREQPHWT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024031
ORF Size 1755 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024031.5
RefSeq Size 2154 bp
RefSeq ORF 1758 bp
Locus ID 78994
UniProt ID Q9BWN1, A0A024QZB5
Cytogenetics 16p11.2
MW 64.3 kDa
Summary The protein encoded by this gene tethers heterochromatin to the nuclear laminar scaffold by binding heterochromatin protein 1 (HP1) and the nuclear lamina. The tether is broken during mitosis and reforms quickly after mitosis, with the encoded protein first binding HP1 and then attaching to the nuclear lamina. This protein also has been shown to promote MyoD activity and skeletal myogenesis. Two transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Feb 2016
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Documents
Resources
"NM_024031" in other vectors (6)
SKU Description Size Price
RC208696L1 Lenti ORF clone of Human proline rich 14 (PRR14), Myc-DDK-tagged 10 ug
$845.00
RC208696L2 Lenti ORF clone of Human proline rich 14 (PRR14), mGFP tagged 10 ug
$845.00
RC208696L3 Lenti ORF clone of Human proline rich 14 (PRR14), Myc-DDK-tagged 10 ug
$845.00
RC208696L4 Lenti ORF clone of Human proline rich 14 (PRR14), mGFP tagged 10 ug
$845.00
RG208696 PRR14 (tGFP-tagged) - Human proline rich 14 (PRR14) 10 ug
$489.00 $745.00
SC321888 PRR14 (untagged)-Human proline rich 14 (PRR14) 10 ug
$545.00

Plasmid Map for RC208696

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208696
Circular map for RC208696

file-alt Citations

The human protein PRR14 tethers heterochromatin to the nuclear lamina during interphase and mitotic exit
Poleshko, Mansfield, Burlingame et al
Cell Rep (2013) 5 (2), 292-301 DOI: 10.1016/j.celrep.2013.09.024
Cited by 135
AI Summary: In this study, OriGene’s PRR14 cDNA clone (RC213307) was used to express and investigate the function of the PRR14 protein, enabling the authors to demonstrate its role in tethering heterochromatin to the nuclear lamina during interphase and mitotic exit.
FigureFigure
The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina during Interphase and Mitotic Exit
Poleshko, Mansfield, Burlingame
Cell Reports (2013) 5 (2), 292
AI Summary: OriGene products, including PRR14 expression vectors with TurboGFP (RG208696), Myc-DDK-tag (RC208696), and an N-terminal mGFP vector (PS100048), were used to study PRR14 localization and function in tethering heterochromatin to the nuclear lamina.
Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).
Showing 1-2 of 2 papers.
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