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CD163 (NM_004244) Human Tagged ORF Clone

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SKU
RC208557
CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,006.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD163
Synonyms M130; MM130; SCARI1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208557 representing NM_004244.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGCAAACTCAGAATGGTGCTACTTGAAGACTCTGGATCTGCTGACTTCAGAAGACATTTTGTCAAC
TTGAGTCCCTTCACCATTACTGTGGTCTTACTTCTCAGTGCCTGTTTTGTCACCAGTTCTCTTGGAGGA
ACAGACAAGGAGCTGAGGCTAGTGGATGGTGAAAACAAGTGTAGCGGGAGAGTGGAAGTGAAAGTCCAG
GAGGAGTGGGGAACGGTGTGTAATAATGGCTGGAGCATGGAAGCGGTCTCTGTGATTTGTAACCAGCTG
GGATGTCCAACTGCTATCAAAGCCCCTGGATGGGCTAATTCCAGTGCAGGTTCTGGACGCATTTGGATG
GATCATGTTTCTTGTCGTGGGAATGAGTCAGCTCTTTGGGATTGCAAACATGATGGATGGGGAAAGCAT
AGTAACTGTACTCACCAACAAGATGCTGGAGTGACCTGCTCAGATGGATCCAATTTGGAAATGAGGCTG
ACGCGTGGAGGGAATATGTGTTCTGGAAGAATAGAGATCAAATTCCAAGGACGGTGGGGAACAGTGTGT
GATGATAACTTCAACATAGATCATGCATCTGTCATTTGTAGACAACTTGAATGTGGAAGTGCTGTCAGT
TTCTCTGGTTCATCTAATTTTGGAGAAGGCTCTGGACCAATCTGGTTTGATGATCTTATATGCAACGGA
AATGAGTCAGCTCTCTGGAACTGCAAACATCAAGGATGGGGAAAGCATAACTGTGATCATGCTGAGGAT
GCTGGAGTGATTTGCTCAAAGGGAGCAGATCTGAGCCTGAGACTGGTAGATGGAGTCACTGAATGTTCA
GGAAGATTAGAAGTGAGATTCCAAGGAGAATGGGGGACAATATGTGATGACGGCTGGGACAGTTACGAT
GCTGCTGTGGCATGCAAGCAACTGGGATGTCCAACTGCCGTCACAGCCATTGGTCGAGTTAACGCCAGT
AAGGGATTTGGACACATCTGGCTTGACAGCGTTTCTTGCCAGGGACATGAACCTGCTGTCTGGCAATGT
AAACACCATGAATGGGGAAAGCATTATTGCAATCACAATGAAGATGCTGGCGTGACATGTTCTGATGGA
TCAGATCTGGAGCTAAGACTTAGAGGTGGAGGCAGCCGCTGTGCTGGGACAGTTGAGGTGGAGATTCAG
AGACTGTTAGGGAAGGTGTGTGACAGAGGCTGGGGACTGAAAGAAGCTGATGTGGTTTGCAGGCAGCTG
GGATGTGGATCTGCACTCAAAACATCTTATCAAGTGTACTCCAAAATCCAGGCAACAAACACATGGCTG
TTTCTAAGTAGCTGTAACGGAAATGAAACTTCTCTTTGGGACTGCAAGAACTGGCAATGGGGTGGACTT
ACCTGTGATCACTATGAAGAAGCCAAAATTACCTGCTCAGCCCACAGGGAACCCAGACTGGTTGGAGGG
GACATTCCCTGTTCTGGACGTGTTGAAGTGAAGCATGGTGACACGTGGGGCTCCATCTGTGATTCGGAC
TTCTCTCTGGAAGCTGCCAGCGTTCTATGCAGGGAATTACAGTGTGGCACAGTTGTCTCTATCCTGGGG
GGAGCTCACTTTGGAGAGGGAAATGGACAGATCTGGGCTGAAGAATTCCAGTGTGAGGGACATGAGTCC
CATCTTTCACTCTGCCCAGTAGCACCCCGCCCAGAAGGAACTTGTAGCCACAGCAGGGATGTTGGAGTA
GTCTGCTCAAGATACACAGAAATTCGCTTGGTGAATGGCAAGACCCCGTGTGAGGGCAGAGTGGAGCTC
AAAACGCTTGGTGCCTGGGGATCCCTCTGTAACTCTCACTGGGACATAGAAGATGCCCATGTTCTTTGC
CAGCAGCTTAAATGTGGAGTTGCCCTTTCTACCCCAGGAGGAGCACGTTTTGGAAAAGGAAATGGTCAG
ATCTGGAGGCATATGTTTCACTGCACTGGGACTGAGCAGCACATGGGAGATTGTCCTGTAACTGCTCTA
GGTGCTTCATTATGTCCTTCAGAGCAAGTGGCCTCTGTAATCTGCTCAGGAAACCAGTCCCAAACACTG
TCCTCGTGCAATTCATCGTCTTTGGGCCCAACAAGGCCTACCATTCCAGAAGAAAGTGCTGTGGCCTGC
ATAGAGAGTGGTCAACTTCGCCTGGTAAATGGAGGAGGTCGCTGTGCTGGGAGAGTAGAGATCTATCAT
GAGGGCTCCTGGGGCACCATCTGTGATGACAGCTGGGACCTGAGTGATGCCCACGTGGTTTGCAGACAG
CTGGGCTGTGGAGAGGCCATTAATGCCACTGGTTCTGCTCATTTTGGGGAAGGAACAGGGCCCATCTGG
CTGGATGAGATGAAATGCAATGGAAAAGAATCCCGCATTTGGCAGTGCCATTCACACGGCTGGGGGCAG
CAAAATTGCAGGCACAAGGAGGATGCGGGAGTTATCTGCTCAGAATTCATGTCTCTGAGACTGACCAGT
GAAGCCAGCAGAGAGGCCTGTGCAGGGCGTCTGGAAGTTTTTTACAATGGAGCTTGGGGCACTGTTGGC
AAGAGTAGCATGTCTGAAACCACTGTGGGTGTGGTGTGCAGGCAGCTGGGCTGTGCAGACAAAGGGAAA
ATCAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACAATGTTCAGTGTCCAAAA
GGACCTGACACGCTGTGGCAGTGCCCATCATCTCCATGGGAGAAGAGACTGGCCAGCCCCTCGGAGGAG
ACCTGGATCACATGTGACAACAAGATAAGACTTCAGGAAGGACCCACTTCCTGTTCTGGACGTGTGGAG
ATCTGGCATGGAGGTTCCTGGGGGACAGTGTGTGATGACTCTTGGGACTTGGACGATGCTCAGGTGGTG
TGTCAACAACTTGGCTGTGGTCCAGCTTTGAAAGCATTCAAAGAAGCAGAGTTTGGTCAGGGGACTGGA
CCGATATGGCTCAATGAAGTGAAGTGCAAAGGGAATGAGTCTTCCTTGTGGGATTGTCCTGCCAGACGC
TGGGGCCATAGTGAGTGTGGGCACAAGGAAGACGCTGCAGTGAATTGCACAGATATTTCAGTGCAGAAA
ACCCCACAAAAAGCCACAACAGGTCGCTCATCCCGTCAGTCATCCTTTATTGCAGTCGGGATCCTTGGG
GTTGTTCTGTTGGCCATTTTCGTCGCATTATTCTTCTTGACTAAAAAGCGAAGACAGAGACAGCGGCTT
GCAGTTTCCTCAAGAGGAGAGAACTTAGTCCACCAAATTCAATACCGGGAGATGAATTCTTGCCTGAAT
GCAGATGATCTGGACCTAATGAATTCCTCAGAAAATTCCCATGAGTCAGCTGATTTCAGTGCTGCTGAA
CTAATTTCTGTGTCTAAATTTCTTCCTATTTCTGGAATGGAAAAGGAGGCCATTCTGAGCCACACTGAA
AAGGAAAATGGGAATTTA
AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>Peptide sequence encoded by RC208557
Blue=ORF Red=Cloning site Green=Tag(s)

MSKLRMVLLEDSGSADFRRHFVNLSPFTITVVLLLSACFVTSSLGGTDKELRLVDGENKCSGRVEVKVQ
EEWGTVCNNGWSMEAVSVICNQLGCPTAIKAPGWANSSAGSGRIWMDHVSCRGNESALWDCKHDGWGKH
SNCTHQQDAGVTCSDGSNLEMRLTRGGNMCSGRIEIKFQGRWGTVCDDNFNIDHASVICRQLECGSAVS
FSGSSNFGEGSGPIWFDDLICNGNESALWNCKHQGWGKHNCDHAEDAGVICSKGADLSLRLVDGVTECS
GRLEVRFQGEWGTICDDGWDSYDAAVACKQLGCPTAVTAIGRVNASKGFGHIWLDSVSCQGHEPAVWQC
KHHEWGKHYCNHNEDAGVTCSDGSDLELRLRGGGSRCAGTVEVEIQRLLGKVCDRGWGLKEADVVCRQL
GCGSALKTSYQVYSKIQATNTWLFLSSCNGNETSLWDCKNWQWGGLTCDHYEEAKITCSAHREPRLVGG
DIPCSGRVEVKHGDTWGSICDSDFSLEAASVLCRELQCGTVVSILGGAHFGEGNGQIWAEEFQCEGHES
HLSLCPVAPRPEGTCSHSRDVGVVCSRYTEIRLVNGKTPCEGRVELKTLGAWGSLCNSHWDIEDAHVLC
QQLKCGVALSTPGGARFGKGNGQIWRHMFHCTGTEQHMGDCPVTALGASLCPSEQVASVICSGNQSQTL
SSCNSSSLGPTRPTIPEESAVACIESGQLRLVNGGGRCAGRVEIYHEGSWGTICDDSWDLSDAHVVCRQ
LGCGEAINATGSAHFGEGTGPIWLDEMKCNGKESRIWQCHSHGWGQQNCRHKEDAGVICSEFMSLRLTS
EASREACAGRLEVFYNGAWGTVGKSSMSETTVGVVCRQLGCADKGKINPASLDKAMSIPMWVDNVQCPK
GPDTLWQCPSSPWEKRLASPSEETWITCDNKIRLQEGPTSCSGRVEIWHGGSWGTVCDDSWDLDDAQVV
CQQLGCGPALKAFKEAEFGQGTGPIWLNEVKCKGNESSLWDCPARRWGHSECGHKEDAAVNCTDISVQK
TPQKATTGRSSRQSSFIAVGILGVVLLAIFVALFFLTKKRRQRQRLAVSSRGENLVHQIQYREMNSCLN
ADDLDLMNSSENSHESADFSAAELISVSKFLPISGMEKEAILSHTEKENGNL
SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Recombinant protein using RC208557 also available, TP308557
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_004244
ORF Size 3468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004244.4
RefSeq Size 4190 bp
RefSeq ORF 3471 bp
Locus ID 9332
UniProt ID Q86VB7
Cytogenetics 12p13.31
Domains SR
Protein Families Druggable Genome, Secreted Protein, Transmembrane
MW 125.4 kDa
Summary The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Aug 2011
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"NM_004244" in other vectors (6)
SKU Description Size Price
RC208557L1 Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, Myc-DDK-tagged 10 ug
$1,306.00
RC208557L2 Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, mGFP tagged 10 ug
$1,306.00
RC208557L3 Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, Myc-DDK-tagged 10 ug
$1,306.00
RC208557L4 Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, mGFP tagged 10 ug
$1,306.00
RG208557 CD163 (tGFP-tagged) - Human CD163 molecule (CD163), transcript variant 1 10 ug
$789.00 $1,206.00
SC117495 CD163 (untagged)-Human CD163 molecule (CD163), transcript variant 1 10 ug
$1,007.00

Plasmid Map for RC208557

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208557
Circular map for RC208557

file-alt Citations

Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions
Alvarado-Vazquez, Bernal, Paige et al
Immunobiology (2017)
AI Summary: OriGene contributed to this study by providing the CD163 and empty plasmid constructs used for transfecting macrophages to investigate the effects of CD163 overexpression on macrophage phenotype under inflammatory conditions.
Snippet: temperature. The plasmids were purchased from Origene (Rockville, MD). Then, 100 μL of the Man-PEI + plasmid preparation were added to primary macrophages or THP-1 macrophages (in 24-well plates, 250,000 cells/well). ... All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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