CD163 (NM_004244) Human Untagged Clone
$1,007.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | CD163 |
| Synonyms | M130; MM130; SCARI1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_004244 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004244.3, NP_004235.3 |
| RefSeq Size | 3806 bp |
| RefSeq ORF | 3471 bp |
| Locus ID | 9332 |
| UniProt ID | Q86VB7 |
| Cytogenetics | 12p13.31 |
| Domains | SR |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Summary | The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Aug 2011 Transcript Variant: This variant (1), also known as long tail variant 1, represents the longer transcript and encodes the longer isoform (a). |
Reviews
Documents
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Resources
"NM_004244" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208557 | CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 1 | 10 ug |
$1,006.00
|
|
| RC208557L1 | Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,306.00
|
|
| RC208557L2 | Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, mGFP tagged | 10 ug |
$1,306.00
|
|
| RC208557L3 | Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,306.00
|
|
| RC208557L4 | Lenti ORF clone of Human CD163 molecule (CD163), transcript variant 1, mGFP tagged | 10 ug |
$1,306.00
|
|
| RG208557 | CD163 (tGFP-tagged) - Human CD163 molecule (CD163), transcript variant 1 | 10 ug |
$789.00
$1,206.00
|
|
Citations
Cytokine production capabilities of human primary monocyte-derived macrophages from patients with diabetes mellitus type 2 with and without diabetic peripheral neuropathy
Alvarado-Vázquez, Grosick, Moracho-Vilrriales et al
J Pain Res (2019) 12, 69-81 DOI: 10.2147/JPR.S186372
Alvarado-Vázquez, Grosick, Moracho-Vilrriales et al
J Pain Res (2019) 12, 69-81 DOI: 10.2147/JPR.S186372
AI Summary: OriGene products contributed to this study by providing the plasmid encoding the CD163 gene (pCD163) and the empty vector control plasmid (pCMV6-XL4), which were used to transfect human primary macrophages and investigate the effects of CD163 induction on their inflammatory phenotype.
Snippet: Macrophages from healthy controls and patients with DM2 or with DPN were transfected as previously described.24 Briefly, 0.5 µg of a plasmid-encoding CD163 gene (pCD163;Origene, Rockville, MD, USA) or an empty vector plasmid as the control (pEmpty;Origene, Rockville, MD, USA) or an empty vector plasmid as the control (pEmpty, pCMV6-XL4 vector;Origene) was mixed with 1 µL of Man-PEI nanoparticle (Polyplus, New York, NY, USA) in a total volume of 100 µL of NaCl solution (150 mM)
CD163 overexpression using a macrophage-directed gene therapy approach improves wound healing in ex vivo and in vivo human skin models
Ferreira, Ulecia-Morón, Alvarado-Vázquez et al
Immunobiology (2019) 225 (1), 151862 DOI: 10.1016/j.imbio.2019.10.011
Ferreira, Ulecia-Morón, Alvarado-Vázquez et al
Immunobiology (2019) 225 (1), 151862 DOI: 10.1016/j.imbio.2019.10.011
Cited by 40
AI Summary: OriGene’s pCMV6-XL4 vector plasmids (pCD163 and pEmpty) were used to transfect macrophages and induce CD163 overexpression, enabling the study of its effects on wound healing in human skin models.
Snippet: … ManPEI was mixed with a cDNA plasmid (pCMV6-XL4 vector, pCD163 or pEmpty, 0.5 μg of plasmid in NaCl, Origene, Rockville, MD) as per manufacturer’s instructions. …Then, after an incubation period of 48 h we performed the CD163 mRNA or protein assessments. Macrophages were added to wound healing preparations at this time point, 48 h after transfection, once CD163 consistently was overexpressed (see below) with our intervention
The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
Yang, Wang, Wang et al
J. Intern. Med. (2017)
Yang, Wang, Wang et al
J. Intern. Med. (2017)
AI Summary: OriGene products, including the recombinant human CD163 protein, CD163 expression plasmid (SC117495, NM_004244.3), and MegaTran 1.0 transfection reagent, were used in this study to investigate the role of CD163 in mediating anti-inflammatory responses to haptoglobin β subunit-HMGB1 complexes.
Identification of CD163 as an antiinflammatory receptor for HMGB1-haptoglobin complexes
Yang, Wang, Levine et al
JCI Insight (2016) 1 (7) DOI: 10.1172/jci.insight.85375
Yang, Wang, Levine et al
JCI Insight (2016) 1 (7) DOI: 10.1172/jci.insight.85375
Cited by 129
A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications
Bover, Cardó-Vila, Kuniyasu et al
J Immunol (2007) 178 (12), 8183-94 DOI: 10.4049/jimmunol.178.12.8183
Bover, Cardó-Vila, Kuniyasu et al
J Immunol (2007) 178 (12), 8183-94 DOI: 10.4049/jimmunol.178.12.8183
Cited by 216
CD163 is the macrophage scavenger receptor for native and chemically modified hemoglobins in the absence of haptoglobin
Schaer, Schaer, Buehler et al
Blood (2006) 107 (1), 373-80 DOI: 10.1182/blood-2005-03-1014
Schaer, Schaer, Buehler et al
Blood (2006) 107 (1), 373-80 DOI: 10.1182/blood-2005-03-1014
Cited by 285
Showing 1-6 of 7 papers.
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