GATA2 (NM_032638) Human Tagged ORF Clone

SKU
RC208554
GATA2 (Myc-DDK-tagged)-Human GATA binding protein 2 (GATA2), transcript variant 2
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$686.00
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Specifications
Product Data
Target Symbol GATA2
Synonyms DCML; IMD21; MONOMAC; NFE1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208554 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGCGCCGGAGCAGCCGCGCTGGATGGCGCACCCGGCCGTGCTGAATGCGCAGCACCCCGACT
CACACCACCCGGGCCTGGCGCACAACTACATGGAACCCGCGCAGCTGCTGCCTCCAGACGAGGTGGACGT
CTTCTTCAATCACCTCGACTCGCAGGGCAACCCCTACTATGCCAACCCCGCTCACGCGCGGGCGCGCGTC
TCCTACAGCCCCGCGCACGCCCGCCTGACCGGAGGCCAGATGTGCCGCCCACACTTGTTGCACAGCCCGG
GTTTGCCCTGGCTGGACGGGGGCAAAGCAGCCCTCTCTGCCGCTGCGGCCCACCACCACAACCCCTGGAC
CGTGAGCCCCTTCTCCAAGACGCCACTGCACCCCTCAGCTGCTGGAGGCCCTGGAGGCCCACTCTCTGTG
TACCCAGGGGCTGGGGGTGGGAGCGGGGGAGGCAGCGGGAGCTCAGTGGCCTCCCTCACCCCTACAGCAG
CCCACTCTGGCTCCCACCTTTTCGGCTTCCCACCCACGCCACCCAAAGAAGTGTCTCCTGACCCTAGCAC
CACGGGGGCTGCGTCTCCAGCCTCATCTTCCGCGGGGGGTAGTGCAGCCCGAGGAGAGGACAAGGACGGC
GTCAAGTACCAGGTGTCACTGACGGAGAGCATGAAGATGGAAAGTGGCAGTCCCCTGCGCCCAGGCCTAG
CTACTATGGGCACCCAGCCTGCTACACACCACCCCATCCCCACCTACCCCTCCTATGTGCCGGCGGCTGC
CCACGACTACAGCAGCGGACTCTTCCACCCCGGAGGCTTCCTGGGGGGACCGGCCTCCAGCTTCACCCCT
AAGCAGCGCAGCAAGGCTCGTTCCTGTTCAGAAGGCCGGGAGTGTGTCAACTGTGGGGCCACAGCCACCC
CTCTCTGGCGGCGGGACGGCACCGGCCACTACCTGTGCAATGCCTGTGGCCTCTACCACAAGATGAATGG
GCAGAACCGACCACTCATCAAGCCCAAGCGAAGACTGTCGGCCGCCAGAAGAGCCGGCACCTGTTGTGCA
AATTGTCAGACGACAACCACCACCTTATGGCGCCGAAACGCCAACGGGGACCCTGTCTGCAACGCCTGTG
GCCTCTACTACAAGCTGCACAATGTTAACAGGCCACTGACCATGAAGAAGGAAGGGATCCAGACTCGGAA
CCGGAAGATGTCCAACAAGTCCAAGAAGAGCAAGAAAGGGGCGGAGTGCTTCGAGGAGCTGTCAAAGTGC
ATGCAGGAGAAGTCATCCCCCTTCAGTGCAGCTGCCCTGGCTGGACACATGGCACCTGTGGGCCACCTCC
CGCCCTTCAGCCACTCCGGACACATCCTGCCCACTCCGACGCCCATCCACCCCTCCTCCAGCCTCTCCTT
CGGCCACCCCCACCCGTCCAGCATGGTGACCGCCATGGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208554 protein sequence
Red=Cloning site Green=Tags(s)

MEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARV
SYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSAAAAHHHNPWTVSPFSKTPLHPSAAGGPGGPLSV
YPGAGGGSGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARGEDKDG
VKYQVSLTESMKMESGSPLRPGLATMGTQPATHHPIPTYPSYVPAAAHDYSSGLFHPGGFLGGPASSFTP
KQRSKARSCSEGRECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTCCA
NCQTTTTTLWRRNANGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKMSNKSKKSKKGAECFEELSKC
MQEKSSPFSAAALAGHMAPVGHLPPFSHSGHILPTPTPIHPSSSLSFGHPHPSSMVTAMG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_032638
ORF Size 1440 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032638.3, NP_116027.2
RefSeq Size 3383 bp
RefSeq ORF 1443 bp
Locus ID 2624
UniProt ID P23769
Cytogenetics 3q21.3
Domains GATA
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
MW 50.5 kDa
Summary This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
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SKU Description Size Price
RC208554L1 Lenti ORF clone of Human GATA binding protein 2 (GATA2), transcript variant 2, Myc-DDK-tagged 10 ug
$986.00
RC208554L2 Lenti ORF clone of Human GATA binding protein 2 (GATA2), transcript variant 2, mGFP tagged 10 ug
$986.00
RC208554L3 Lenti ORF clone of Human GATA binding protein 2 (GATA2), transcript variant 2, Myc-DDK-tagged 10 ug
$986.00
RC208554L4 Lenti ORF clone of Human GATA binding protein 2 (GATA2), transcript variant 2, mGFP tagged 10 ug
$986.00
RG208554 GATA2 (tGFP-tagged) - Human GATA binding protein 2 (GATA2), transcript variant 2 10 ug
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SC125368 GATA2 (untagged)-Human GATA binding protein 2 (GATA2), transcript variant 2 10 ug
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SC321007 GATA2 (untagged)-Human GATA binding protein 2 (GATA2), transcript variant 2 10 ug
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Citations

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