KRT34 (NM_021013) Human Tagged ORF Clone

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SKU
RC208312
KRT34 (Myc-DDK-tagged)-Human keratin 34 (KRT34)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRT34
Synonyms Ha-4; HA4; hHa4; KRTHA4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208312 representing NM_021013
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTATGCCAAGCCCCCACCCACAATTAATGGTATAAAAGGACTGCAGAGGAAGGAGAGACTCAAAC
CTGCCCACATCCACCTCCAGCAGCTTACCTGCTTTTCCATTACCTGTTCCAGCACCATGTCTTACAGTTG
TTGCCTGCCCAGCCTGGGCTGCCGCACCAGCTGCTCCTCCCGGCCCTGCGTGCCCCCCAGCTGCCACGGC
TACACCCTGCCTGGGGCCTGCAACATCCCCGCCAATGTGAGCAACTGCAACTGGTTCTGTGAGGGCTCCT
TCAATGGCAGCGAGAAGGAGACTATGCAGTTCCTGAACGACCGCCTGGCCAGCTACCTGGAGAAGGTGCG
TCAGCTGGAGCGGGACAACGCGGAGCTGGAGAAACTCATCCAGGAGCGGTCCCAGCAGCAGGAGCCCTTG
CTGTGCCCCAGCTACCAGTCGTACTTCAAGACCATTGAGGAGCTCCAGCAGAAGATTCTGTGTGCCAAGG
CTGAGAATGCCAGGCTGGTGGTGAACATTGACAATGCCAAGCTGGCCTCTGACGACTTCAGAAGCAAGTA
CCAGACGGAGCAGTCCCTGAGGCTGTTGGTGGAGTCGGACATCAACAGCATACGCAGGATCCTGGATGAG
CTGACCCTCTGCAAGTCTGACCTGGAGTCCCAGGTGGAGTCCCTGAGGGAGGAGCTGATCTGCTTGAAGA
AGAACCATGAGGAGGAGGTTAACACCCTGCGCTCCCAGCTTGGAGACCGCCTCAACGTGGAGGTGGACAC
TGCCCCCACTGTGGACCTGAACCAGGTCCTGAACGAGACCAGGAGTCAGTATGAGGCTCTGGTGGAAACT
AACCGCAGGGAAGTGGAGCAATGGTTCGCCACGCAGACCGAGGAGCTGAACAAGCAGGTGGTATCCAGCT
CAGAGCAGCTGCAGTCCTGCCAGGCGGAGATCATCGAGCTGAGACGCACAGTCAACGCCCTGGAGATCGA
GCTGCAGGCCCAGCACAACCTGCGAGACTCTCTGGAAAACACGCTGACGGAGAGCGAGGCCCACTACAGC
TCCCAGCTGTCCCAGGTGCAGAGCCTGATCACCAACGTGGAGTCTCAGCTGGCAGAGATCCGCTGTGACC
TGGAGCGGCAGAACCAGGAGTACCAGGTGCTGCTGGACGTGCGTGCCCGGCTGGAGTGTGAGATCAACAC
GTACCGGAGCCTCCTGGAGAGTGAGGACTGCAAGCTCCCCTGCAACCCATGCGCCACCACCAATGCTAGT
GGCAACTCCTGTGGACCCTGTGGCACCTCTCAAAAGGGTTGCTGTAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208312 representing NM_021013
Red=Cloning site Green=Tags(s)

MLYAKPPPTINGIKGLQRKERLKPAHIHLQQLTCFSITCSSTMSYSCCLPSLGCRTSCSSRPCVPPSCHG
YTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELEKLIQERSQQQEPL
LCPSYQSYFKTIEELQQKILCAKAENARLVVNIDNAKLASDDFRSKYQTEQSLRLLVESDINSIRRILDE
LTLCKSDLESQVESLREELICLKKNHEEEVNTLRSQLGDRLNVEVDTAPTVDLNQVLNETRSQYEALVET
NRREVEQWFATQTEELNKQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHNLRDSLENTLTESEAHYS
SQLSQVQSLITNVESQLAEIRCDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPCNPCATTNAS
GNSCGPCGTSQKGCCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021013
ORF Size 1308 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021013.3, NP_066293.2
RefSeq Size 1713 bp
RefSeq ORF 1311 bp
Locus ID 3885
UniProt ID O76011
Cytogenetics 17q21.2
MW 49.2 kDa
Summary The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_021013" in other vectors (4)
SKU Description Size Price
RC208312L3 Lenti ORF clone of Human keratin 34 (KRT34), Myc-DDK-tagged 10 ug
$757.00
RC208312L4 Lenti ORF clone of Human keratin 34 (KRT34), mGFP tagged 10 ug
$757.00
RG208312 KRT34 (tGFP-tagged) - Human keratin 34 (KRT34) 10 ug
$489.00 $657.00
SC126360 KRT34 (untagged)-Human keratin 34 (KRT34) 10 ug
$457.00

Plasmid Map for RC208312

Download sequence for

Cloning scheme for RC208312

Circular map for RC208312

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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