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SYS1 (NM_033542) Human Tagged ORF Clone

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SKU
RC208279
SYS1 (Myc-DDK-tagged)-Human SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (SYS1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "SYS1 or NM_033542"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SYS1
Synonyms C20orf169; dJ453C12.4; dJ453C12.4.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208279 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGTCAGTTCCGCAGCTACGTGTGGGACCCGCTGCTGATCCTGTCGCAGATCGTCCTCATGCAGA
CCGTGTATTACGGCTCGCTGGGCCTGTGGCTGGCGCTGGTGGACGGGCTAGTGCGAAGCAGCCCCTCGCT
GGACCAGATGTTCGACGCCGAGATCCTGGGCTTTTCCACCCCTCCAGGCCGGCTCTCCATGATGTCCTTC
ATCCTCAACGCCCTCACCTGTGCCCTGGGCTTGCTGTACTTCATCCGGCGAGGAAAGCAGTGTCTGGATT
TCACTGTCACTGTCCATTTCTTTCACCTCCTGGGCTGCTGGTTCTACAGCTCCCGTTTCCCCTCGGCGCT
GACCTGGTGGCTGGTCCAAGCCGTGTGCATTGCACTCATGGCTGTCATCGGGGAGTACCTGTGCATGCGG
ACGGAGCTCAAGGAGATACCCCTCAACTCAGCCCCTAAATCCAATGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208279 protein sequence
Red=Cloning site Green=Tags(s)

MAGQFRSYVWDPLLILSQIVLMQTVYYGSLGLWLALVDGLVRSSPSLDQMFDAEILGFSTPPGRLSMMSF
ILNALTCALGLLYFIRRGKQCLDFTVTVHFFHLLGCWFYSSRFPSALTWWLVQAVCIALMAVIGEYLCMR
TELKEIPLNSAPKSNV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033542
ORF Size 468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033542.4
RefSeq Size 2802 bp
RefSeq ORF 471 bp
Locus ID 90196
UniProt ID Q8N2H4
Cytogenetics 20q13.12
Protein Families Transmembrane
MW 17.6 kDa
Summary SYS1 forms a complex with ADP-ribosylation factor-related protein ARFRP1 (MIM 604699) and targets ARFRP1 to the Golgi apparatus (Behnia et al., 2004 PubMed 15077113).supplied by OMIM, Aug 2009
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"NM_033542" in other vectors (4)
SKU Description Size Price
RC208279L3 Lenti ORF clone of Human SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (SYS1), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC208279L4 Lenti ORF clone of Human SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (SYS1), transcript variant 1, mGFP tagged 10 ug
$450.00
RG208279 SYS1 (tGFP-tagged) - Human SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (SYS1), transcript variant 1 10 ug
$489.00
SC111350 SYS1 (untagged)-Human SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (SYS1), transcript variant 1 10 ug
$150.00

Plasmid Map for RC208279

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208279
Circular map for RC208279

file-alt Citations

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