LYRM7 (NM_181705) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC208223
LYRM7 (Myc-DDK-tagged)-Human Lyrm7 homolog (mouse) (LYRM7)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LYRM7
Synonyms C5orf31; MC3DN8; MZM1L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208223 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACGGGCAGTCAAGGTTTTACAGCTCTTTAAAACACTGCACAGGACCAGACAACAAGTTTTTAAAA
ATGATGCCAGAGCATTAGAAGCAGCCAGAATAAAGATAAATGAAGAATTCAAAAATAATAAAAGTGAAAC
TTCTTCTAAGAAAATAGAAGAGCTAATGAAAATAGGTTCTGATATTGAATTATTACTCAGAACATCTGTT
ATACAAGGTATTCACACAGACCACAATACACTGAAACTGGTCCCTAGGAAAGACCTTCTTGTAGAAAATG
TGCCATATTGTGATGCACCAACTCAGAAGCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208223 protein sequence
Red=Cloning site Green=Tags(s)

MGRAVKVLQLFKTLHRTRQQVFKNDARALEAARIKINEEFKNNKSETSSKKIEELMKIGSDIELLLRTSV
IQGIHTDHNTLKLVPRKDLLVENVPYCDAPTQKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181705
ORF Size 312 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181705.1, NP_859056.1
RefSeq Size 6247 bp
RefSeq ORF 315 bp
Locus ID 90624
UniProt ID Q5U5X0
Cytogenetics 5q23.3-q31.1
MW 12 kDa
Summary Inner mitochondrial membrane complex III (CIII) is the main enzyme complex in the mitochondrial respiratory chain, and Rieske Fe-S protein (UQCRFS1) is the last catalytic subunit added to the complex. The protein encoded by this gene is a nuclear-encoded mitochondrial matrix protein that stabilizes UQCRFS1 and chaperones it to the CIII complex. Defects in this gene are a cause of mitochondrial complex III deficiency, nuclear type 8. Three transcript variants encoding two different isoforms have been found for this gene. provided by RefSeq, Jun 2014
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_181705" in other vectors (5)
SKU Description Size Price
RC208223L3 Lenti-ORF clone of LYRM7 (Myc-DDK-tagged)-Human Lyrm7 homolog (mouse) (LYRM7) 10 ug
$450.00
RC208223L4 Lenti-ORF clone of LYRM7 (mGFP-tagged)-Human Lyrm7 homolog (mouse) (LYRM7) 10 ug
$450.00
RG208223 LYRM7 (tGFP-tagged) - Human Lyrm7 homolog (mouse) (LYRM7) 10 ug
$350.00
SC124565 LYRM7 (untagged)-Human Lyrm7 homolog (mouse) (LYRM7) 10 ug
$150.00
SC322099 LYRM7 (untagged)-Human Lyrm7 homolog (mouse) (LYRM7) 10 ug
$150.00

Plasmid Map for RC208223

Download sequence for

Cloning scheme for RC208223

Circular map for RC208223

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products