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FECH (NM_000140) Human Tagged ORF Clone

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SKU
RC208058
FECH (Myc-DDK-tagged)-Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug 10 ug
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Other products for "FECH or NM_000140"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FECH
Synonyms EPP; EPP1; FCE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208058 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTTCACTCGGCGCAAACATGGCTGCGGCCCTGCGCGCCGCGGGCGTCCTGCTCCGCGATCCGCTGG
CATCCAGCAGCTGGAGGGTCTGTCAGCCATGGAGGTGGAAGTCAGGTGCAGCTGCAGCGGCCGTCACCAC
AGAAACAGCCCAGCATGCCCAGGGTGCAAAACCTCAAGTTCAACCGCAGAAGAGGAAGCCGAAAACTGGA
ATATTAATGCTAAACATGGGAGGCCCTGAAACTCTTGGAGATGTTCACGACTTCCTTCTGAGACTCTTCT
TGGACCGAGACCTCATGACACTTCCTATTCAGAATAAGCTGGCACCATTCATCGCCAAACGCCGAACCCC
CAAGATTCAAGAGCAGTACCGCAGGATTGGAGGCGGATCCCCCATCAAGATATGGACTTCCAAGCAGGGA
GAGGGCATGGTGAAGCTGCTGGATGAATTGTCCCCCAACACAGCCCCTCACAAATACTATATTGGATTTC
GGTACGTCCATCCTTTAACAGAAGAAGCAATTGAAGAGATGGAGAGAGATGGCCTAGAAAGGGCTATTGC
TTTCACACAGTATCCACAGTACAGCTGCTCCACCACAGGCAGCAGCTTAAATGCCATTTACAGATACTAT
AATCAAGTGGGACGGAAGCCCACGATGAAGTGGAGCACTATTGACAGGTGGCCCACACATCACCTCCTCA
TCCAGTGCTTTGCAGATCATATTCTAAAGGAACTGGACCATTTTCCACTTGAGAAGAGAAGCGAGGTGGT
CATTCTGTTTTCTGCTCACTCACTGCCCATGTCTGTGGTCAACAGAGGCGACCCATATCCTCAGGAGGTA
AGCGCCACTGTCCAAAAAGTCATGGAAAGGCTGGAGTACTGCAACCCCTACCGACTGGTGTGGCAATCCA
AGGTTGGTCCAATGCCCTGGTTGGGTCCTCAAACAGACGAATCTATCAAAGGGCTTTGTGAGAGGGGGAG
GAAGAATATCCTCTTGGTTCCGATAGCATTTACCAGTGACCATATTGAAACGCTGTATGAGCTGGACATC
GAGTACTCTCAAGTTTTAGCCAAGGAGTGTGGAGTTGAAAACATCAGAAGAGCTGAGTCTCTTAATGGAA
ATCCATTGTTCTCTAAGGCCCTGGCCGACTTGGTGCATTCACACATCCAGTCAAACGAGCTGTGTTCCAA
GCAGCTGACCCTGAGCTGTCCGCTCTGTGTCAATCCTGTCTGCAGGGAGACTAAATCCTTCTTCACCAGC
CAGCAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208058 protein sequence
Red=Cloning site Green=Tags(s)

MRSLGANMAAALRAAGVLLRDPLASSSWRVCQPWRWKSGAAAAAVTTETAQHAQGAKPQVQPQKRKPKTG
ILMLNMGGPETLGDVHDFLLRLFLDRDLMTLPIQNKLAPFIAKRRTPKIQEQYRRIGGGSPIKIWTSKQG
EGMVKLLDELSPNTAPHKYYIGFRYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYY
NQVGRKPTMKWSTIDRWPTHHLLIQCFADHILKELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPYPQEV
SATVQKVMERLEYCNPYRLVWQSKVGPMPWLGPQTDESIKGLCERGRKNILLVPIAFTSDHIETLYELDI
EYSQVLAKECGVENIRRAESLNGNPLFSKALADLVHSHIQSNELCSKQLTLSCPLCVNPVCRETKSFFTS
QQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000140
ORF Size 1269 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000140.5
RefSeq Size 7277 bp
RefSeq ORF 1272 bp
Locus ID 2235
UniProt ID P22830
Cytogenetics 18q21.31
Domains Ferrochelatase
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Porphyrin and chlorophyll metabolism
MW 47.9 kDa
Summary The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.provided by RefSeq, May 2010
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Documents
Resources
"NM_000140" in other vectors (6)
SKU Description Size Price
RC208058L1 Lenti ORF clone of Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC208058L2 Lenti ORF clone of Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged 10 ug
$757.00
RC208058L3 Lenti ORF clone of Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC208058L4 Lenti ORF clone of Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged 10 ug
$757.00
RG208058 FECH (tGFP-tagged) - Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$489.00 $657.00
SC125310 FECH (untagged)-Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$457.00

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