FECH (NM_000140) Human Untagged Clone

SKU SC125310

FECH (untagged)-Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: FECH
• Synonyms: EPP; EPP1; FCE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125310 sequence for NM_000140 edited (data generated by NextGen Sequencing); ATGCGTTCACTCGGCGCAAACATGGCTGCGGCCCTGCGCGCCGCGGGCGTCCTGCTCCGC; GATCCGCTGGCATCCAGCAGCTGGAGGGTCTGTCAGCCATGGAGGTGGAAGTCAGGTGCA; GCTGCAGCGGCCGTCACCACAGAAACAGCCCAGCATGCCCAGGGTGCAAAACCTCAAGTT; CAACCGCAGAAGAGGAAGCCGAAAACTGGAATATTAATGCTAAACATGGGAGGCCCTGAA; ACTCTTGGAGATGTTCACGACTTCCTTCTGAGACTCTTCTTGGACCGAGACCTCATGACA; CTTCCTATTCAGAATAAGCTGGCACCATTCATCGCCAAACGCCGAACCCCCAAGATTCAA; GAGCAGTACCGCAGGATTGGAGGCGGATCCCCCATCAAGATATGGACTTCCAAGCAGGGA; GAGGGCATGGTGAAGCTGCTGGATGAATTGTCCCCCAACACAGCCCCTCACAAATACTAT; ATTGGATTTCGGTACGTCCATCCTTTAACAGAAGAAGCAATTGAAGAGATGGAGAGAGAT; GGCCTAGAAAGGGCTATTGCTTTCACACAGTATCCACAGTACAGCTGCTCCACCACAGGC; AGCAGCTTAAATGCCATTTACAGATACTATAATCAAGTGGGACGGAAGCCCACGATGAAG; TGGAGCACTATTGACAGGTGGCCCACACATCACCTCCTCATCCAGTGCTTTGCAGATCAT; ATTCTAAAGGAACTGGACCATTTTCCACTTGAGAAGAGAAGCGAGGTGGTCATTCTGTTT; TCTGCTCACTCACTGCCCATGTCTGTGGTCAACAGAGGCGACCCATATCCTCAGGAGGTA; AGCGCCACTGTCCAAAAAGTCATGGAAAGGCTGGAGTACTGCAACCCCTACCGACTGGTG; TGGCAATCCAAGGTTGGTCCAATGCCCTGGTTGGGTCCTCAAACAGACGAATCTATCAAA; GGGCTTTGTGAGAGGGGGAGGAAGAATATCCTCTTGGTTCCGATAGCATTTACCAGTGAC; CATATTGAAACGCTGTATGAGCTGGACATCGAGTACTCTCAAGTTTTAGCCAAGGAGTGT; GGAGTTGAAAACATCAGAAGAGCTGAGTCTCTTAATGGAAATCCATTGTTCTCTAAGGCC; CTGGCCGACTTGGTGCATTCACACATCCAGTCAAACGAGCTGTGTTCCAAGCAGCTGACC; CTGAGCTGTCCGCTCTGTGTCAATCCTGTCTGCAGGGAGACTAAATCCTTCTTCACCAGC; CAGCAGCTGTGA; ; Clone variation with respect to NM_000140.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000140 unedited; TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAAT; TCGGCACGAGGGGAGCCCGGACTCGGGCCGAGGCTGCCCAGGCAATGCGTTCACTCGGCG; CAAACATGGCTGCGGCCCTGCGCGCCGCGGGCGTCCTGCTCCGCGATCCGCTGGCATCCA; GCAGCTGGAGGGTCTGTCAGCCATGGAGGTGGAAGTCAGGTGCAGCTGCAGCGGCCGTCA; CCACAGAAACAGCCCAGCATGCCCAGGGTGCAAAACCTCAAGTTCAACCGCAGAAGAGGA; AGCCGAAAACTGGAATATTAATGCTAAACATGGGAGGCCCTGAAACTCTTGGAGATGTTC; ACGACTTCCTTCTGAGACTCTTCTTGGACCGAGACCTCATGACACTTCCTATTCAGAATA; AGCTGGCACCATTCATCGCCAAACGCCGAACCCCCAAGATTCAAGAGCAGTACCGCAGGA; TTGGAGGCGGATCCCCCATCAAGATATGGACTTCCAAGCAGGGAGAGGGCATGGTGAAGC; TGCTGGATGAATTGTCCCCCAACACAGCCCCTCACAAATACTATATTGGATTTCGGTACG; TCCATCCTTTAACAGAAGAAGCAATTGAAGAGATGGAGAGAGATGGCCTAGAAAGGGCTA; TTGCTNTCACACAGTATCCACAGTACAGCTGCTCCACCACAGGCAGCAGCTTAAATGCCA; TTTACAGATACTATAATCAAGTGGGACGGAAGCCCACGATGAAGTGGAGCACTATTGACA; GGTGGGCCACACATNNACTNCTCATNCAGTGCTTTGCAGATCATATTCTAAAGAACTGGA; CATTTTNCACTTGAGAGAGAAGCGAGGTGTCATTCTGTTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000140 unedited; CTATGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGACATTATTTCT; ATATTTTTAGAAACACTTTTCACTCATAAATCCAAGGTGACAATGGCATTAAATGGCATT; AAAAATATCTACAGGAAAGGACTACCACGAAATACAATATTATGATAACAGTTATTGCAT; TAAAGTGGTTACTTTTCTGTATTCTTCACATGTCCCATTGTATCACTATACATTTTTATA; ATAAAAAATCGTAAATTAAGAAAGAATTCAAGCAAAGTATTGTTGACTTTATAAAATAAT; TCTTAAAACAAATGTTCAGAAAGAAGGCATTCAACTAAGATAGAGAAGATGCTGGATGGA; GAGGCTTGCAAGGTCCCGGGAGGAGCTCCAGGGTGAAGCTCATCACTAGCCTTTCCAGCA; GCACTGTCCCTGGAGACCAGAAGCAGTGGGAGCCCCTTCCCTGTGGCACCACGGACTCCC; ACCAAGGCACGTATCATGAACACTGTACTTGTACACGTTTATTACCCTTGGGTGTATGGG; GAATCTAAGAGCACCTCTGAAATCCATACTTGATTCCTTTTTACATTGAGGAAAGTGAGA; TTCACACACAAATACCAAGATGACCAATGAATGGTGGCCTCGCTGGCAAAAGCAGCCATT; GCAGGTAACCAAATTGCCTCCCTAGCATTCTGTAACCCATGGGCTTAAGGAAAAATTAAG; TAACATTTCTTTTTCAAATTCATTTCAAAAACTCAACTGTGTTCATTTTAAAAACAGCAA; CTATATTGCCCGAAACTAAACAGATTTCATTCACACTGCCAGCCCACAGAGGGGACTCTC; CCTACCCTTTAAGAAGGGTTTTGGCCAATAAACCCAAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000140
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000140.2, NP_000131.2
• RefSeq Size: 3817 bp
• RefSeq ORF: 1272 bp
• Locus ID: 2235
• UniProt ID: P22830
• Cytogenetics: 18q21.31
• Domains: Ferrochelatase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism
• Summary: The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.[provided by RefSeq, May 2010]; Transcript Variant: This variant (2) uses an alternate in-frame donor splice site at one of the internal coding exons, compared to transcript variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.