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ABHD1 (NM_032604) Human Tagged ORF Clone

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SKU
RC208020
ABHD1 (Myc-DDK-tagged)-Human abhydrolase domain containing 1 (ABHD1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ABHD1
Synonyms LABH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208020 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGCTCCTTCCTGAGCCCCCAGAATGGCACCTGGGCAGACACCTTCTCTCTGCTCTTGGCTCTTG
CCGTTGCCCTCTACTTGGGCTACTACTGGGCATGTGTGCTTCAGAGGCCTCGGCTGGTGGCTGGGCCGCA
GTTTCTGGCCTTCCTGGAGCCACACTGTTCCATCACCACCGAGACTTTCTACCCAACGCTGTGGTGTTTT
GAGGGGCGACTACAAAGCATCTTCCAAGTCCTCCTGCAGTCTCAGCCCCTAGTCCTTTATCAGAGTGACA
TCCTCCAAACACCAGATGGAGGCCAGCTCCTGCTAGACTGGGCCAAGCAGCCTGACAGCAGCCAAGACCC
TGATCCTACTACCCAGCCCATTGTGCTGCTGCTTCCTGGCATCACTGGCAGTAGCCAGGAGACATACGTC
TTGCACCTAGTTAACCAAGCTCTGAGGGATGGCTACCAGGCTGTCGTGTTTAACAACCGGGGCTGCCGTG
GGGAGGAACTGCGGACCCACAGGGCTTTTTGTGCCAGCAATACTGAAGATCTAGAGACAGTCGTGAACCA
CATAAAGCATCGTTATCCCCAAGCTCCACTGCTGGCCGTGGGCATCTCTTTTGGAGGGATACTGGTGCTG
AATCACCTGGCACAGGCCAGGCAGGCTGCAGGGCTGGTGGCAGCACTGACTCTGTCTGCATGCTGGGATT
CCTTTGAGACCACTCGCTCCCTGGAAACCCCACTCAACTCACTGCTCTTCAATCAGCCCCTCACTGCTGG
GCTCTGCCAACTTGTGGAACGAAACAGAAAGGTGATTGAAAAGGTGGTGGACATAGACTTTGTACTACAG
GCCCGTACAATCCGCCAGTTTGATGAGCGCTACACATCTGTGGCCTTTGGATATCAAGACTGTGTTACCT
ACTACAAAGCAGCAAGCCCTAGAACCAAGATAGATGCCATCCGGATCCCTGTGCTCTATCTCAGTGCAGC
AGATGACCCCTTCTCCCCCGTCTGTGCCCTTCCCATACAGGCCGCCCAACACTCCCCCTACGTTGCGCTG
CTCATCACAGCCCGGGGTGGCCACATCGGCTTCCTGGAAGGGCTGCTCCCGTGGCAGCACTGGTACATGA
GCCGCCTCTTGCATCAGTACGCCAAAGCCATCTTCCAGGACCCAGAGGGGCTGCCTGACCTCAGGGCTCT
CTTACCTTCTGAGGACAGAAACAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208020 protein sequence
Red=Cloning site Green=Tags(s)

MLSSFLSPQNGTWADTFSLLLALAVALYLGYYWACVLQRPRLVAGPQFLAFLEPHCSITTETFYPTLWCF
EGRLQSIFQVLLQSQPLVLYQSDILQTPDGGQLLLDWAKQPDSSQDPDPTTQPIVLLLPGITGSSQETYV
LHLVNQALRDGYQAVVFNNRGCRGEELRTHRAFCASNTEDLETVVNHIKHRYPQAPLLAVGISFGGILVL
NHLAQARQAAGLVAALTLSACWDSFETTRSLETPLNSLLFNQPLTAGLCQLVERNRKVIEKVVDIDFVLQ
ARTIRQFDERYTSVAFGYQDCVTYYKAASPRTKIDAIRIPVLYLSAADDPFSPVCALPIQAAQHSPYVAL
LITARGGHIGFLEGLLPWQHWYMSRLLHQYAKAIFQDPEGLPDLRALLPSEDRNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032604
ORF Size 1215 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032604.4
RefSeq Size 1471 bp
RefSeq ORF 1218 bp
Locus ID 84696
UniProt ID Q96SE0
Cytogenetics 2p23.3
Protein Families Transmembrane
MW 45.2 kDa
Summary This gene is a member of the AB hydrolase superfamily and encodes a protein with an alpha/beta hydrolase fold. This domain is common to a number of hydrolytic enzymes of widely differing phylogenetic origins and catalytic functions. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_032604" in other vectors (4)
SKU Description Size Price
RC208020L3 Lenti ORF clone of Human abhydrolase domain containing 1 (ABHD1), Myc-DDK-tagged 10 ug
$757.00
RC208020L4 Lenti ORF clone of Human abhydrolase domain containing 1 (ABHD1), mGFP tagged 10 ug
$757.00
RG208020 ABHD1 (tGFP-tagged) - Human abhydrolase domain containing 1 (ABHD1) 10 ug
$489.00 $657.00
SC125958 ABHD1 (untagged)-Human abhydrolase domain containing 1 (ABHD1) 10 ug
$457.00

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