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HSPC111 (NOP16) (NM_016391) Human Tagged ORF Clone

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SKU
RC208009
NOP16 (Myc-DDK-tagged)-Human NOP16 nucleolar protein homolog (yeast) (NOP16)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSPC111
Synonyms HSPC111; HSPC185
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208009 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAGGCCAAGGGCAAAACCCGGAGGCAGAAGTTTGGTTACAGTGTCAACCGAAAGCGTCTGAACC
GGAATGCTCGACGGAAGGCAGCGCCGCGGATCGAATGCTCCCACATCCGACATGCCTGGGACCACGCTAA
ATCGGTACGGCAGAACCTGGCCGAGATGGGGTTGGCTGTGGACCCCAACAGGGCGGTGCCCCTCCGTAAG
AGAAAGGTGAAGGCCATGGAGGTGGACATAGAGGAGAGGCCTAAAGAGCTTGTACGGAAGCCCTATGTGC
TGAATGACCTGGAGGCAGAAGCCAGCCTTCCAGAAAAGAAAGGAAATACTCTGTCTCGGGACCTCATTGA
CTATGTACGCTACATGGTAGAGAACCACGGGGAGGACTATAAGGCCATGGCCCGTGATGAGAAGAATTAC
TATCAAGATACCCCAAAACAGATTCGGAGTAAGATCAACGTCTATAAACGCTTTTACCCAGCAGAGTGGC
AAGACTTCCTCGATTCTTTGCAGAAGAGGAAGATGGAGGTGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208009 protein sequence
Red=Cloning site Green=Tags(s)

MPKAKGKTRRQKFGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPLRK
RKVKAMEVDIEERPKELVRKPYVLNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMARDEKNY
YQDTPKQIRSKINVYKRFYPAEWQDFLDSLQKRKMEVE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016391
ORF Size 534 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016391.8
RefSeq Size 1071 bp
RefSeq ORF 537 bp
Locus ID 51491
UniProt ID Q9Y3C1
Cytogenetics 5q35.2
Protein Families Druggable Genome
MW 21.2 kDa
Summary This gene encodes a protein that is localized to the nucleolus. Expression of this gene is induced by estrogens and Myc protein and is a marker of poor patient survival in breast cancer. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2015
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"NM_016391" in other vectors (4)
SKU Description Size Price
RC208009L3 Lenti ORF clone of Human NOP16 nucleolar protein homolog (yeast) (NOP16), Myc-DDK-tagged 10 ug
$600.00
RC208009L4 Lenti ORF clone of Human NOP16 nucleolar protein homolog (yeast) (NOP16), mGFP tagged 10 ug
$600.00
RG208009 NOP16 (tGFP-tagged) - Human NOP16 nucleolar protein homolog (yeast) (NOP16) 10 ug
$489.00 $500.00
SC126120 NOP16 (untagged)-Human NOP16 nucleolar protein homolog (yeast) (NOP16) 10 ug
$300.00

Plasmid Map for RC208009

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208009
Circular map for RC208009

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