HSPC111 (NOP16) (NM_016391) Human Untagged Clone

SKU SC126120

NOP16 (untagged)-Human NOP16 nucleolar protein homolog (yeast) (NOP16)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HSPC111
• Synonyms: HSPC111; HSPC185
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016391 edited; GAGAGACAGAGGCAGCGTGTTTGAGCTGCTGGTGCGGTGGTCAGCGCGATGCCCAAGGCC; AAGGGCAAAACCCGGAGGCAGAAGTTTGGTTACAGTGTCAACCGAAAGCGTCTGAACCGG; AATGCTCGACGGAAGGCAGCGCCGCGGATCGAATGCTCCCACATCCGACATGCCTGGGAC; CACGCTAAATCGGTACGGCAGAACCTGGCCGAGATGGGGTTGGCTGTGGACCCCAACAGG; GCGGTGCCCCTCCGTAAGAGAAAGGTGAAGGCCATGGAGGTGGACATAGAGGAGAGGCCT; AAAGAGCTTGTACGGAAGCCCTATGTGCTGAATGACCTGGAGGCAGAAGCCAGCCTTCCA; GAAAAGAAAGGAAATACTCTGTCTCGGGACCTCATTGACTATGTACGCTACATGGTAGAG; AACCACGGGGAGGACTATAAGGCCATGGCCCGTGATGAGAAGAATTACTATCAAGATACC; CCAAAACAGATTCGGAGTAAGATCAACGTCTATAAACGCTTTTACCCAGCAGAGTGGCAA; GACTTCCTCGATTCTTTGCAGAAGAGGAAGATGGAGGTGGAGTGACTGGTTTACATCACA; GCTGCCCCAGGCTGAGGCGTCCCCCGGACCAGTGAAGCTGGAGCCAGGGTGTAAGGCAAG; GAGGTGCTGTGTGGCTCCAGAGGAGCTGGCCAGGTCCCATGGAATCAGAAGGTTACACAC; ACACGTGCACACTCCCCGCTCTGGGGAAGGAACTGTTCTCAGAGGCTCCAATTTATATTC; ATCTGGGGGTTCACGGAAAAGCCAGAACCTGCTGTTTTCAGGGTGGGTGATGTAAATATA; GTGTGTACATAATAAAGCAAATATATTTTACTTCTCTAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016391 unedited; GGGGTTCAGATTTTGTAATACGACTTACTATAGGGCGGCCGCGCATTCAGATCTGGTACC; GGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGAGAGACAGAGGCAGCGTGTT; TGAGCTGCTGGTGCGGTGGTCAGCGCGATGCCCAAGGCCAAGGGCAAAACCCGGAGGCAG; AAGTTTGGTTACAGTGTCAACCGAAAGCGTCTGAACCGGAATGCTCGACGGAAGGCAGCG; CCGCGGATCGAATGCTCCCACATCCGACATGCCTGGGACCACGCTAAATCGGTACGGCAG; AACCTGGCCGAGATGGGGTTGGCTGTGGACCCCAACAGGGCGGTGCCCCTCCGTAAGAGA; AAGGTGAAGGCCATGGAGGTGGACATAGAGGAGAGGCCTAAAGAGCTTGTACGGAAGCCC; TATGTGCTGAATGACCTGGAGGCAGAAGCCAGCCTTCCAGAAAAGAAAGGAAATACTCTG; TCTCGGGACCTCATTGACTATGTACGCTACATGGTAGAGAACCACGGGGAGGACTATAAG; GCCATGGCCCGTGATGAGAAGAATTACTATCAAGATACCCCAAAACAGATTCGGAGTAAG; ATCAACGTCTATAAACGCTTTTACCCAGCAGAGTGGCAAGACTTCCTCGATTCTTTGCAG; AAGAGGAAGATGGAGGTGGAGTGACTGGTTTACATCACAGCTGCCCCAGGCTGANGCGTC; CCCCGGACCAGTGAAGCTGGAGCCAGGGTGTAAGGCAAGGAGGTGCTGTGTGGCTCCAGA; GGAGCTGGGCCGGTCCCATGGAATCAGAANGNTACACACACACGTGCACACTCCCCGCTC; TGGGGAAAGGACTGNNTCTCAGAGCTCCAATTTATATTCATCTGGGGGTTCACGGAAAAG; CCAGACCTGN;
• Restriction Sites: NotI-NotI
• ACCN: NM_016391
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016391.2, NP_057475.1
• RefSeq Size: 1280 bp
• RefSeq ORF: 537 bp
• Locus ID: 51491
• UniProt ID: Q9Y3C1
• Cytogenetics: 5q35.2
• Protein Families: Druggable Genome
• Summary: This gene encodes a protein that is localized to the nucleolus. Expression of this gene is induced by estrogens and Myc protein and is a marker of poor patient survival in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]; Transcript Variant: This variant (3) uses an alternate splice in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.