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CREG2 (NM_153836) Human Tagged ORF Clone

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SKU
RC207985
CREG2 (Myc-DDK-tagged)-Human cellular repressor of E1A-stimulated genes 2 (CREG2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CREG2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207985 representing NM_153836
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGTGCGCCGCGGCCGGCGGCCGGCGCGGCCGGGGACCCGCCTCTCCTGGCTGCTGTGCTGCAGCG
CCCTGCTGTCCCCGGCCGCGGGCTACGTGATCGTGAGCTCCGTGTCTTGGGCCGTCACCAACGAGGTGGA
CGAGGAGCTGGACAGCGCCTCCACTGAGGAGGCTATGCCCGCGCTGCTAGAGGATTCGGGCAGCATCTGG
CAGCAAAGCTTCCCCGCCTCTGCCCACAAGGAGGACGCGCACCTGCGGCCCCGGGCGGGCGCCGCCCGGG
CCAGGCCGCCCCCCGCGCCACCCGGGATGTTCTCCTACCGGCGCGAGGGCGGCCAGACGGCCAGTGCGCC
CCCGGGCCCTAGACTGCGCGCCGCCACCGCCCGCTCCCTGGCCCATGCCAGCGTCTGGGGCTGCCTGGCC
ACCGTGTCCACCCACAAGAAGATCCAAGGACTGCCATTTGGGAACTGCCTGCCCGTCAGTGATGGCCCCT
TCAACAATAGCACTGGGATTCCTTTCTTCTACATGACAGCCAAGGACCCCGTGGTGGCTGATCTGATGAA
GAACCCCATGGCCTCGCTGATGCTGCCAGAATCAGAAGGGGAGTTCTGCAGAAAAAACATCGTTGATCCG
GAAGATCCCCGATGTGTCCAGTTAACGCTCACTGGCCAGATGATCGCAGTGTCTCCAGAAGAAGTAGAAT
TTGCCAAGCAAGCCATGTTTTCAAGGCACCCAGGGATGAGGAAGTGGCCTCGTCAATATGAATGGTTCTT
TATGAAGATGAGGATAGAACATATCTGGCTTCAGAAATGGTATGGAGGCGCATCCAGTATTTCAAGGGAG
GAATATTTCAAAGCAGTTCCCAGAAAGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207985 representing NM_153836
Red=Cloning site Green=Tags(s)

MSVRRGRRPARPGTRLSWLLCCSALLSPAAGYVIVSSVSWAVTNEVDEELDSASTEEAMPALLEDSGSIW
QQSFPASAHKEDAHLRPRAGAARARPPPAPPGMFSYRREGGQTASAPPGPRLRAATARSLAHASVWGCLA
TVSTHKKIQGLPFGNCLPVSDGPFNNSTGIPFFYMTAKDPVVADLMKNPMASLMLPESEGEFCRKNIVDP
EDPRCVQLTLTGQMIAVSPEEVEFAKQAMFSRHPGMRKWPRQYEWFFMKMRIEHIWLQKWYGGASSISRE
EYFKAVPRKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153836
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153836.4
RefSeq Size 3201 bp
RefSeq ORF 873 bp
Locus ID 200407
UniProt ID Q8IUH2
Cytogenetics 2q11.2
Protein Families Secreted Protein
MW 31.9 kDa
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Documents
Resources
"NM_153836" in other vectors (4)
SKU Description Size Price
RC207985L3 Lenti ORF clone of Human cellular repressor of E1A-stimulated genes 2 (CREG2), Myc-DDK-tagged 10 ug
$600.00
RC207985L4 Lenti ORF clone of Human cellular repressor of E1A-stimulated genes 2 (CREG2), mGFP tagged 10 ug
$600.00
RG207985 CREG2 (tGFP-tagged) - Human cellular repressor of E1A-stimulated genes 2 (CREG2) 10 ug
$500.00
SC100549 CREG2 (untagged)-Human cellular repressor of E1A-stimulated genes 2 (CREG2) 10 ug
$300.00

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