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Pirh2 (RCHY1) (NM_015436) Human Tagged ORF Clone

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SKU
RC207970
RCHY1 (Myc-DDK-tagged)-Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Pirh2 or NM_015436"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Pirh2
Synonyms ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY; ZNF363
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207970 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGACGGCCCGGGAAGATGGCGCCAGCGGTCAAGAGCGAGGTCAGCGGGGCTGCGAGCACTATG
ACAGAGGATGTCTCCTAAAGGCACCTTGCTGTGACAAGCTTTATACTTGCCGCTTGTGTCATGATAACAA
TGAAGATCATCAACTAGATCGCTTTAAAGTGAAGGAAGTGCAGTGCATAAACTGTGAAAAAATTCAACAT
GCCCAACAGACTTGTGAAGAATGTAGCACATTGTTTGGAGAATATTATTGCGATATATGCCATTTGTTTG
ACAAAGATAAGAAGCAGTATCACTGTGAAAACTGTGGAATTTGTAGGATTGGTCCAAAGGAAGATTTTTT
CCATTGTTTGAAATGTAACTTATGCCTAGCTATGAATCTTCAAGGAAGACACAAGTGTATTGAAAATGTG
TCCCAACAGAATTGTCCAATATGTTTGGAGGACATTCACACATCCCGTGTTGTTGCTCATGTCTTGCCAT
GTGGACATCTTTTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGCTACAGATGTCCATTATGTAT
GCACTCTGCTTTAGATATGACCAGGTATTGGAGACAGCTGGATGATGAAGTAGCACAGACTCCTATGCCA
TCAGAATATCAGAACATGACTGTGGATATTCTCTGCAATGACTGTAATGGACGATCCACTGTTCAGTTTC
ATATATTAGGCATGAAATGTAAGATTTGTGAATCCTATAATACTGCTCAAGCTGGAGGACGTAGAATTTC
ACTGGATCAGCAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207970 protein sequence
Red=Cloning site Green=Tags(s)

MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQH
AQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENV
SQQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMP
SEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015436
ORF Size 783 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015436.3
RefSeq Size 4447 bp
RefSeq ORF 786 bp
Locus ID 25898
UniProt ID Q96PM5
Cytogenetics 4q21.1
Domains RING
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways p53 signaling pathway, Ubiquitin mediated proteolysis
MW 30.1 kDa
Summary The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Jun 2013
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"NM_015436" in other vectors (6)
SKU Description Size Price
RC207970L1 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC207970L2 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, mGFP tagged 10 ug
$600.00
RC207970L3 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC207970L4 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG207970 RCHY1 (tGFP-tagged) - Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 10 ug
$489.00 $500.00
SC107968 RCHY1 (untagged)-Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 10 ug
$300.00

Plasmid Map for RC207970

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207970
Circular map for RC207970

file-alt Citations

E3 ligase RCHY1 negatively regulates HDAC2
Choi, Choi, An et al
Biochem. Biophys. Res. Commun. (2019) 521 (1), 37-41 DOI: 10.1016/j.bbrc.2019.10.049
AI Summary: OriGene’s RCHY1 human tagged ORF clone (RC207970) was used to generate a FLAG-tagged RCHY1 construct for studying the regulation of HDAC2 by RCHY1 in this paper.
Snippet: …pcDNA3-2x FLAG-tagged RCHY1 was generated using RCHY1 human tagged ORF clone (Origene, RC207970) by subcloning using BamHI and XhoI sites;- MG132, cycloheximide and VPA were purchased from Sigma. … pcDNA3-2x FLAG-tagged RCHY1 was generated using RCHY1 human tagged ORF clone (Origene, RC207970) by subcloning using BamHI and XhoI;
Showing 1-1 of 1 papers.
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