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Pirh2 (RCHY1) (NM_015436) Human Untagged Clone

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SKU
SC107968
RCHY1 (untagged)-Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Pirh2
Synonyms ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY; ZNF363
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC107968 sequence for NM_015436 edited (data generated by NextGen Sequencing)
ATGGCGGCGACGGCCCGGGAAGATGGCGCCAGCGGTCAAGAGCGAGGTCAGCGGGGCTGC
GAGCACTATGACAGAGGATGTCTCCTAAAGGCACCTTGCTGTGACAAGCTTTATACTTGC
CGCTTGTGTCATGATAACAATGAAGATCATCAACTAGATCGCTTTAAAGTGAAGGAAGTG
CAGTGCATAAACTGTGAAAAAATTCAACATGCCCAACAGACTTGTGAAGAATGTAGCACA
TTGTTTGGAGAATATTATTGCGATATATGCCATTTGTTTGACAAAGATAAGAAGCAGTAT
CACTGTGAAAACTGTGGAATTTGTAGGATTGGTCCAAAGGAAGATTTTTTCCATTGTTTG
AAATGTAACTTATGCCTAGCTATGAATCTTCAAGGAAGACACAAGTGTATTGAAAATGTG
TCCCGACAGAATTGTCCAATATGTTTGGAGGACATTCACACATCCCGTGTTGTTGCTCAT
GTCTTGCCATGTGGACATCTTTTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGC
TACAGATGTCCATTATGTATGCACTCTGCTTTAGATATGACCAGGTATTGGAGACAGCTG
GATGATGAAGTAGCACAGACTCCTATGCCATCAGAATATCAGAACATGACTGTGGATATT
CTCTGCAATGACTGTAATGGACGATCCACTGTTCAGTTTCATATATTAGGCATGAAATGT
AAGATTTGTGAATCCTATAATACTGCTCAAGCTGGAGGACGTAGAATTTCACTGGATCAG
CAATGA

Clone variation with respect to NM_015436.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_015436 unedited
GCCGCTGAATTCGCACGAGGGTGGAATGTTATGGGAGGTGGAGGAGATGGCGGCGACGGC
CCGGGAAGATGGCGCCAGCGGTCAAGAGCGAGGTCAGCGGGGCTGCGAGCACTATGACAG
AGGATGTCTCCTAAAGGCACCTTGCTGTGACAAGCTTTATACTTGCCGCTTGTGTCATGA
TAACAATGAAGATCATCAACTAGATCGCTTTAAAGTGAAGGAAGTGCAGTGCATAAACTG
TGAAAAAAATTCAACATGCCCAACAGACTTGTGAAGAATGTAGCACATTGTTTGGAGAAT
ATTATTGCGATATATGCCATTTGTTTGACAAAGATAAGAAGCAGTATCACTGTGAAAACT
GTGGAATTTGTAGGATTGGTCCAAAGGAAGATTTTTTCCATTGTTTGAAATGTAACTTAT
GCCTAGCTATGAATCTTCAAGGAAGACACAAGTGTATTGAAAATGTGTCCCGACAGAATT
GTCCAATATGTTTGGAGGACATTCACACATCCCGTGTTGTTGCTCATGTCTTGCCATGTG
GACATCTTTTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGCTACAGATGTCCAT
TATGTATGCACTCTGCTTTAGATATGACCAGGTATTGGAGACAGCTGGATGATGAAGTAG
CACAGACTCCTATGCCATCAGAATATCAGAACATGACTGTGGATATTCTCTGCAATGAAC
TGTATGGACGATCCACTGTTCAGTTTCATATATTANGCATGAAAATGTAAGATTTGTGAA
TTCTATTATACTGCTCAAGCTTGGAGACCTAGAATTTCACTGGATCAGCAATGACGACCC
TCACAGTACTGGAGAACTTGCAATTTCTGATTTATAAAAAGCTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_015436 unedited
TCACCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTGGTTTT
TTTACCACAAAAAAGGTATGAGGCAATAAATTTATTACATTGATTTAATCATTCTACACT
GTAAACATATATCAAAACATCACATTGTACTCCATATATATAATATACACAATTATCAAT
TAAATTTTAATAACACAAAGGACAAAAGAAAAATAAAGTGGCATAGTTTTGAACAACTCC
CATTTAAAATTAATTCATTTCAAATTAAACTATTCAACTAGTGAATACCTATGGCCAGGC
ACTTATAAAATAATGTTGGCTCTTTAGTACTTAGTAAATTTATTTTGATGCATGACCAAA
ATAAAAGGGAAATTTAACATGTATATCATATGAAAATAGGTTTTAGTGAAGAAATAAAAA
CTGAGTTTGATTTTAAAATCCATGACACTATAAACAAACTACTACTTCAAACATAAGCTA
AAGTTAATTCATAAAGAGTTCTATGTACTGGTTAAGAAGACCCCAGAAACTAAGAAATGA
ACCACTTGGTATACACTCATTTTTTTTTTATACATACATCTTTAATGATAAAAGAAACTG
AGATGCTATGCACCAAAAAATTCCTAAGGGGATTGGTATTTTACCTTTTTTAAAAATAAT
CCCAATAAACTATAGATACAAATTCTTGCGCAGGGCAACCCTACTTATTTCAGGGACAGA
ATATTCTAGTGTACTTAAGAACTGTCTAAGTAATTAACAATCCCTGGAGCCTTTTAAAAA
GAATAGGAAAAGAAGAAGATGTCTGTTAAAATGGTAACAGTAGTTCTCTCTGAAAGAAGG
NATGTTGAGAGATTTTTACCTTCTTCTTTTTGNAGGATAAACTTTCCTATTGAGTCCGAA
AAGGAAAAAAATTANGGTAAAGTTTTGATTTTAATTTT
Restriction Sites NotI-NotI |
ACCN NM_015436
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015436.2, NP_056251.2
RefSeq Size 4336 bp
RefSeq ORF 786 bp
Locus ID 25898
UniProt ID Q96PM5
Cytogenetics 4q21.1
Domains RING
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways p53 signaling pathway, Ubiquitin mediated proteolysis
Summary The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Jun 2013
Transcript Variant: This variant (1) encodes the longest isoform (1, also known as Pirh2A).
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Documents
Resources
"NM_015436" in other vectors (6)
SKU Description Size Price
RC207970 RCHY1 (Myc-DDK-tagged)-Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 10 ug
$300.00
RC207970L1 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC207970L2 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, mGFP tagged 10 ug
$600.00
RC207970L3 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC207970L4 Lenti ORF clone of Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG207970 RCHY1 (tGFP-tagged) - Human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 10 ug
$489.00 $500.00

file-alt Citations

Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis
Xu, Chen, Wang et al
Cancer Manag Res (2019) 11, 7577-7585 DOI: 10.2147/CMAR.S210825
AI Summary: OriGene products, specifically the si-YAP1 small interfering RNA (No. SR323110) and the OE-RCHY1 overexpression plasmid (No. SC107968), were used to manipulate YAP1 and RCHY1 expression in glioma cells, enabling the study of their roles in radiation-induced apoptosis.
Snippet: To delete the expression of YAP1, glioma cells were transfected with the small interfering RNAs (si-YAP1) purchased from OriGene (No. SR323110;Beijing, China). Meanwhile, the plasmid used to overexpress RCHY1 (OE-RCHY1) in U251 cells was also obtained from OriGene (No. SC107968)
FigureFigureFigure
Showing 1-1 of 1 papers.
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