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DTX3L (NM_138287) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC207944
DTX3L (Myc-DDK-tagged)-Human deltex 3-like (Drosophila) (DTX3L)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $678.00
5 Days*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA375644 DTX3L Rabbit polyclonal Antibody
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TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
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Other products for "DTX3L or NM_138287"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DTX3L
Synonyms BBAP; RNF143
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCCACCTGCGCCCGCCGTCCCCGCTCCTCGTGCGGGTGTACAAGTCCGGCCCCCGAGTACGAA
GGAAGCTGGAGAGCTACTTCCAGAGCTCTAAGTCCTCGGGCGGCGGGGAGTGCACGGTCAGCACCCAGGA
ACACGAAGCCCCGGGCACCTTCCGGGTGGAGTTCAGTGAAAGGGCAGCTAAGGAGAGAGTGTTGAAAAAA
GGAGAGCACCAAATACTTGTTGACGAAAAACCTGTGCCCATTTTCCTGGTACCCACTGAAAATTCAATAA
AGAAGAACACGAGACCTCAAATTTCTTCACTGACACAATCACAAGCAGAAACACCGTCTGGTGATATGCA
TCAACATGAAGGACATATTCCTAATGCTGTGGATTCCTGTCTCCAAAAGATCTTTCTTACTGTAACAGCT
GACCTGAACTGTAACCTGTTCTCCAAAGAGCAGAGGGCATACATAACCACACTGTGCCCTAGTATCAGAA
AAATGGAAGGTCACGATGGAATTGAGAAGGTGTGTGGTGACTTCCAAGACATTGAAAGAATACATCAATT
TTTGAGTGAGCAGTTCCTGGAAAGTGAGCAGAAACAACAATTTTCCCCTTCAATGACAGAGAGGAAGCCA
CTCAGTCAGCAGGAGAGGGACAGCTGCATTTCTCCTTCTGAACCAGAAACCAAGGCAGAACAAAAAAGCA
ACTATTTTGAAGTTCCCTTGCCTTACTTTGAATACTTTAAATATATCTGTCCTGATAAAATCAACTCAAT
AGAGAAAAGATTTGGTGTAAACATTGAAATCCAGGAGAGTTCTCCAAATATGGTCTGTTTAGATTTCACC
TCAAGTCGATCAGGTGACCTGGAAGCAGCTCGTGAGTCTTTTGCTAGTGAATTTCAGAAGAACACAGAAC
CTCTGAAGCAAGAATGTGTCTCTTTAGCAGACAGTAAGCAGGCAAATAAATTCAAACAGGAATTGAATCA
CCAGTTTACAAAGCTCCTTATAAAGGAGAAAGGAGGCGAATTAACTCTCCTTGGGACCCAAGATGACATT
TCAGCTGCCAAACAAAAAATCTCTGAAGCTTTTGTCAAGATACCTGTGAAACTATTTGCTGCCAATTACA
TGATGAATGTAATTGAGGTTGATAGTGCCCACTATAAACTTTTAGAAACTGAATTACTACAGGAGATATC
AGAGATCGAAAAAAGGTATGACATTTGCAGCAAGGTTTCTGAGAAAGGTCAGAAAACCTGCATTCTGTTT
GAATCCAAGGACAGGCAGGTAGATCTATCTGTGCATGCTTATGCAAGTTTCATCGATGCCTTTCAACATG
CCTCATGTCAGTTGATGAGAGAAGTTCTTTTACTGAAGTCTTTGGGCAAGGAGAGAAAGCACTTACATCA
GACCAAGTTTGCTGATGACTTTAGAAAAAGACATCCAAATGTACACTTTGTGCTAAATCAAGAGTCAATG
ACTTTGACTGGTTTGCCAAATCACCTTGCAAAGGCGAAGCAGTATGTTCTAAAAGGAGGAGGAATGTCTT
CATTGGCTGGAAAGAAATTGAAAGAGGGTCATGAAACACCGATGGACATTGATAGCGATGATTCCAAAGC
AGCTTCTCCGCCACTCAAGGGCTCTGTGAGTTCTGAGGCCTCAGAACTGGACAAGAAGGAAAAGGGCATC
TGTGTCATCTGTATGGACACCATTAGTAACAAAAAAGTGCTACCAAAGTGCAAGCATGAATTCTGCGCCC
CTTGTATCAACAAAGCCATGTCATATAAGCCAATCTGTCCCACATGCCAGACTTCCTATGGTATTCAGAA
AGGAAATCAGCCAGAGGGAAGCATGGTTTTCACTGTTTCAAGAGACTCACTTCCAGGTTATGAGTCCTTT
GGCACCATTGTGATTACTTATTCTATGAAAGCAGGCATACAAACAGAAGAACACCCAAACCCAGGAAAGA
GATACCCTGGAATACAGCGAACTGCATACTTGCCTGATAATAAGGAAGGAAGGAAGGTTTTGAAACTGCT
TTATAGGGCCTTTGACCAAAAGCTGATTTTTACAGTGGGGTACTCTCGCGTATTAGGAGTCTCAGATGTC
ATCACTTGGAATGATATTCACCACAAAACATCCCGGTTTGGAGGACCAGAAATGTATGGCTATCCTGATC
CTTCTTACCTGAAACGTGTCAAAGAGGAGCTGAAAGCCAAAGGAATTGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207944 protein sequence
Red=Cloning site Green=Tags(s)

MASHLRPPSPLLVRVYKSGPRVRRKLESYFQSSKSSGGGECTVSTQEHEAPGTFRVEFSERAAKERVLKK
GEHQILVDEKPVPIFLVPTENSIKKNTRPQISSLTQSQAETPSGDMHQHEGHIPNAVDSCLQKIFLTVTA
DLNCNLFSKEQRAYITTLCPSIRKMEGHDGIEKVCGDFQDIERIHQFLSEQFLESEQKQQFSPSMTERKP
LSQQERDSCISPSEPETKAEQKSNYFEVPLPYFEYFKYICPDKINSIEKRFGVNIEIQESSPNMVCLDFT
SSRSGDLEAARESFASEFQKNTEPLKQECVSLADSKQANKFKQELNHQFTKLLIKEKGGELTLLGTQDDI
SAAKQKISEAFVKIPVKLFAANYMMNVIEVDSAHYKLLETELLQEISEIEKRYDICSKVSEKGQKTCILF
ESKDRQVDLSVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESM
TLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGI
CVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESF
GTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDV
ITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138287
ORF Size 2220 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138287.3, NP_612144.1
RefSeq Size 5768 bp
RefSeq ORF 2223 bp
Locus ID 151636
UniProt ID Q8TDB6
Cytogenetics 3q21.1
Domains RING
Protein Families Druggable Genome
Protein Pathways Notch signaling pathway
MW 83.6 kDa
Summary DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 PubMed 12670957).supplied by OMIM, Nov 2009
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Documents
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"NM_138287" in other vectors (6)
SKU Description Size Price
RC207944L1 Lenti ORF clone of Human deltex 3-like (Drosophila) (DTX3L), Myc-DDK-tagged 10 ug
$978.00
RC207944L2 Lenti ORF clone of Human deltex 3-like (Drosophila) (DTX3L), mGFP tagged 10 ug
$978.00
RC207944L3 Lenti ORF clone of Human deltex 3-like (Drosophila) (DTX3L), Myc-DDK-tagged 10 ug
$978.00
RC207944L4 Lenti ORF clone of Human deltex 3-like (Drosophila) (DTX3L), mGFP tagged 10 ug
$978.00
RG207944 DTX3L (tGFP-tagged) - Human deltex 3-like (Drosophila) (DTX3L) 10 ug
$789.00 $878.00
SC311147 DTX3L (untagged)-Human deltex 3-like (Drosophila) (DTX3L) 10 ug
$746.00

Plasmid Map for RC207944

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207944
Circular map for RC207944

file-alt Citations

DTX3L Inhibits the EMT, Metastasis, and Stem-Like Features of Gastric Cancer Through Promoting GSK-3β Dependent SNAI1 Decay
Chen, Li, Shen et al
Adv Sci (Weinh) (2026)
AI Summary: OriGene products pCMV6-DTX3L-FLAG (RC207944) and pCMV6-Entry Vector (PS100001) were used in this study to manipulate DTX3L expression in gastric cancer cells, enabling the authors to investigate DTX3L’s role in inhibiting EMT, metastasis, and stem-like features.
Snippet: pCMV6-DTX3L-FLAG (RC207944) and pCMV6-Entry Vector (PS100001) were obtained from OriGene. pcDNA3.1-SNAI1-HA (#31697) was obtained from Addgene. The mutants of pcDNA3.1-DTX3L/SNAI1 were obtained from Sangon
Showing 1-1 of 1 papers.
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