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EBF1 (NM_024007) Human Tagged ORF Clone

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SKU
RC207836
EBF1 (Myc-DDK-tagged)-Human early B-cell factor 1 (EBF1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $825.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EBF1
Synonyms COE1; EBF; O/E-1; OLF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207836 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCAGCATGAAGGAAGAGCCGCTGGGCAGCGGCA
TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACGGCGGCGCAGAGCGGGGT
GGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCCAATCTGCGGAAATCCAACTTCTTCCACTTC
GTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATCGAGAGGACAGCGTTTGTGGGGTTCGTGG
AGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAACGGAATTCACTACCGGCTTCAGCTTCTCTACAG
CAATGGGATAAGGACGGAGCAGGATTTCTACGTGCGCCTCATTGACTCCATGACAAAACAAGCCATAGTG
TATGAAGGCCAAGACAAGAACCCAGAAATGTGCCGAGTCTTGCTCACACATGAGATCATGTGCAGCCGCT
GTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTGACAGGTTCTT
CTTGAAATTTTTCCTCAAATGTAACCAAAATTGCCTAAAGAATGCGGGAAACCCACGTGACATGCGGAGA
TTCCAGGTCGTGGTGTCTACGACAGTCAATGTGGATGGCCATGTCCTGGCAGTCTCTGATAACATGTTTG
TCCATAATAATTCCAAGCATGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACGCCCTCTTATCT
GGAACATGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGTGCGACTGTG
ATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGT
TGATCACTCCTCATGCCATCCGTGTGCAGACCCCTCCTCGGCACATCCCTGGTGTTGTGGAAGTCACACT
GTCCTACAAATCTAAGCAGTTCTGCAAAGGAACACCAGGCAGATTCATTTATACAGCGCTCAACGAACCC
ACCATCGATTATGGTTTCCAGAGGTTACAGAAGGTCATTCCTCGGCACCCTGGTGACCCTGAGCGTTTGC
CAAAGGAAGTAATACTCAAAAGGGCTGCGGATCTGGTAGAAGCACTGTATGGGATGCCACACAACAACCA
GGAAATCATTCTGAAGAGAGCGGCCGACATTGCCGAGGCCCTGTACAGTGTTCCCCGCAACCACAACCAA
CTCCCGGCCCTTGCTAACACCTCGGTCCACGCAGGGATGATGGGCGTGAATTCGTTCAGTGGACAACTGG
CCGTGAATGTCTCCGAGGCATCACAAGCCACCAATCAGGGTTTCACCCGCAACTCAAGCAGCGTATCACC
ACACGGGTACGTGCCGAGCACCACTCCCCAGCAGACCAACTATAACTCCGTCACCACGAGCATGAACGGA
TACGGCTCTGCCGCAATGTCCAATTTGGGCGGCTCCCCCACCTTCCTCAACGGCTCAGCTGCCAACTCCC
CCTATGCCATAGTGCCATCCAGCCCCACCATGGCCTCCTCCACAAGCCTCCCCTCCAACTGCAGCAGCTC
CTCGGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCCGTGAAACAGAAGAGTGCTTTCGCACCA
GTCGTCAGACCCCAGACCTCCCCACCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATAT
CTGGCATGATTGTTCCTCCTATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207836 protein sequence
Red=Cloning site Green=Tags(s)

MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
VLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIV
YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRR
FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATV
IIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP
TIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQ
LPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNG
YGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAP
VVRPQTSPPPTCTSTNGNSLQAISGMIVPPM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024007
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024007.5
RefSeq Size 5288 bp
RefSeq ORF 1776 bp
Locus ID 1879
UniProt ID Q9UH73
Cytogenetics 5q33.3
Protein Families Transcription Factors
MW 64.5 kDa
Summary Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024007" in other vectors (7)
SKU Description Size Price
RC207836L1 Lenti ORF clone of Human early B-cell factor 1 (EBF1), Myc-DDK-tagged 10 ug
$1,125.00
RC207836L2 Lenti ORF clone of Human early B-cell factor 1 (EBF1), mGFP tagged 10 ug
$1,125.00
RC207836L3 Lenti ORF clone of Human early B-cell factor 1 (EBF1), Myc-DDK-tagged 10 ug
$1,125.00
RC207836L4 Lenti ORF clone of Human early B-cell factor 1 (EBF1), mGFP tagged 10 ug
$1,125.00
RG207836 EBF1 (tGFP-tagged) - Human early B-cell factor 1 (EBF1) 10 ug
$489.00 $1,025.00
SC107271 EBF1 (untagged)-Human early B-cell factor 1 (EBF1) 10 ug
$826.00
SC322605 EBF1 (untagged)-Human early B-cell factor 1 (EBF1) 10 ug
$826.00

Plasmid Map for RC207836

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207836
Circular map for RC207836

file-alt Citations

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