CEBPE (NM_001805) Human Tagged ORF Clone

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SKU
RC207802
CEBPE (Myc-DDK-tagged)-Human CCAAT/enhancer binding protein (C/EBP), epsilon (CEBPE)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CEBPE
Synonyms C/EBP-epsilon; c/EBP epsilon; CRP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207802 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCACGGGACCTACTACGAGTGTGAGCCCCGGGGTGGCCAGCAGCCACTCGAGTTCTCAGGGGGCC
GAGCTGGGCCCGGGGAGCTAGGGGACATGTGTGAGCATGAGGCCTCCATTGACCTCTCCGCCTACATCGA
GTCTGGGGAAGAGCAGCTTCTCTCCGATCTCTTTGCCGTGAAGCCAGCGCCTGAGGCCAGAGGCCTCAAG
GGCCCCGGAACCCCTGCCTTCCCCCACTACTTGCCGCCTGACCCTCGGCCCTTTGCCTACCCTCCACATA
CCTTCGGCCCAGACAGGAAGGCGCTGGGGCCTGGCATCTACAGCAGCCCAGGGAGCTACGACCCCAGGGC
TGTGGCGGTGAAGGAGGAGCCCCGGGGGCCAGAGGGCAGCCGAGCTGCCAGCCGAGGCAGCTACAATCCC
CTGCAGTACCAAGTGGCACACTGTGGGCAGACAGCCATGCACCTGCCCCCAACTCTGGCAGCACCCGGCC
AGCCTCTGCGCGTTCTCAAGGCCCCTTTGGCCACTGCCGCACCCCCCTGCAGTCCCCTCCTGAAGGCGCC
CTCCCCGGCTGGCCCCTTACACAAGGGCAAGAAGGCAGTGAACAAAGATAGCCTTGAGTACCGGCTGAGG
CGGGAGCGCAACAACATCGCCGTGCGCAAGAGCCGAGACAAGGCCAAGAGGCGCATTCTGGAGACGCAGC
AGAAGGTGCTGGAGTACATGGCAGAGAACGAGCGCCTCCGCAGCCGCGTGGAGCAGCTCACCCAGGAGCT
AGACACCCTCCGCAACCTCTTCCGCCAGATTCCTGAGGCGGCCAACCTCATCAAGGGCGTGGGGGGTTGC
AGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207802 protein sequence
Red=Cloning site Green=Tags(s)

MSHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLK
GPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPRGPEGSRAASRGSYNP
LQYQVAHCGQTAMHLPPTLAAPGQPLRVLKAPLATAAPPCSPLLKAPSPAGPLHKGKKAVNKDSLEYRLR
RERNNIAVRKSRDKAKRRILETQQKVLEYMAENERLRSRVEQLTQELDTLRNLFRQIPEAANLIKGVGGC
S

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001805
ORF Size 843 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001805.4
RefSeq Size 1550 bp
RefSeq ORF 846 bp
Locus ID 1053
UniProt ID Q15744
Cytogenetics 14q11.2
Protein Families Druggable Genome, Transcription Factors
MW 30.6 kDa
Summary The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001805" in other vectors (6)
SKU Description Size Price
RC207802L1 Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), epsilon (CEBPE), Myc-DDK-tagged 10 ug
$600.00
RC207802L2 Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), epsilon (CEBPE), mGFP tagged 10 ug
$600.00
RC207802L3 Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), epsilon (CEBPE), Myc-DDK-tagged 10 ug
$600.00
RC207802L4 Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), epsilon (CEBPE), mGFP tagged 10 ug
$600.00
RG207802 CEBPE (tGFP-tagged) - Human CCAAT/enhancer binding protein (C/EBP), epsilon (CEBPE) 10 ug
$489.00 $500.00
SC125641 CEBPE (untagged)-Human CCAAT/enhancer binding protein (C/EBP), epsilon (CEBPE) 10 ug
$300.00

Plasmid Map for RC207802

Download sequence for

Cloning scheme for RC207802

Circular map for RC207802

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