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SEC24D (NM_014822) Human Tagged ORF Clone

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SKU
RC207747
SEC24D (Myc-DDK-tagged)-Human SEC24 family, member D (S. cerevisiae) (SEC24D)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $899.00
In Stock*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each 100 pmol
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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug 10 ug
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AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL 5 ml
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Other products for "SEC24D or NM_014822"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SEC24D
Synonyms CLCRP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207747 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCAACAAGGTTACGTGGCTACACCTCCGTATTCTCAGCCTCAGCCTGGAATAGGCCTTTCTCCAC
CTCATTATGGGCACTATGGGGATCCGTCGCACACAGCATCTCCAACAGGTATGATGAAGCCAGCAGGGCC
TTTGGGGGCCACCGCCACTAGGGGAATGTTGCCTCCGGGTCCCCCACCTCCTGGACCCCATCAGTTTGGT
CAGAATGGAGCTCATGCCACTGGTCACCCTCCCCAAAGATTTCCAGGCCCTCCACCTGTCAACAATGTGG
CATCCTCACATGCACCATACCAACCCTCTGCACAATCTTCTTATCCAGGTCCTATATCCACTTCATCTGT
CACCCAGCTGGGCAGCCAGCTCAGTGCTATGCAAATCAACAGCTATGGTTCAGGCATGGCTCCTCCAAGC
CAGGGACCCCCTGGCCCTCTGTCAGCCACATCATTGCAGACTCCTCCACGACCTCCACAGCCTTCCATTT
TGCAGCCTGGATCTCAAGTTCTTCCACCACCACCCACCACACTCAATGGTCCTGGTGCCTCACCTTTGCC
TCTACCAATGTACAGACCAGATGGGCTCTCTGGGCCTCCTCCTCCAAATGCCCAGTACCAGCCCCCACCT
CTTCCAGGCCAGACCTTGGGTGCTGGATATCCTCCGCAGCAGGCAGCCAACTCTGGTCCCCAGATGGCAG
GCGCACAACTGTCTTACCCAGGAGGCTTCCCTGGAGGTCCTGCACAGATGGCTGGTCCGCCACAGCCCCA
GAAGAAGCTGGATCCTGACTCTATCCCTAGCCCAATCCAGGTGATTGAGAATGATAGAGCCAGCAGAGGA
GGACAAGTTTATGCCACCAACACCAGAGGCCAGATCCCTCCCCTGGTCACTACAGATTGCATGATACAAG
ACCAAGGAAATGCCAGTCCTCGATTCATCCGTTGTACAACATACTGTTTTCCATGCACGTCAGATATGGC
TAAGCAAGCTCAGATTCCATTAGCTGCTGTCATCAAGCCCTTTGCCACCATTCCTTCAAATGAGAGTCCC
CTTTACTTGGTAAATCACGGCGAGAGTGGACCAGTCAGATGCAACAGGTGCAAGGCCTACATGTGCCCAT
TTATGCAGTTCATCGAAGGAGGAAGGAGATATCAGTGTGGATTTTGCAACTGTGTGAATGATGTTCCACC
ATTCTATTTCCAACATCTGGACCACATTGGAAGAAGACTGGACCACTATGAGAAACCAGAGTTATCTCTA
GGATCTTATGAATATGTTGCCACTTTGGATTATTGCAGAAAGAGTAAGCCTCCCAACCCACCAGCCTTTA
TCTTCATGATTGATGTTTCATATAGTAACATAAAGAATGGACTTGTCAAGCTCATATGTGAAGAACTGAA
GACCATGCTGGAAAAAATTCCAAAGGAAGAGCAAGAAGAGACGTCTGCAATTCGAGTGGGTTTTATCACA
TATAACAAAGTTCTCCATTTCTTTAATGTGAAGAGTAATCTGGCCCAGCCTCAGATGATGGTGGTGACTG
ATGTTGGAGAAGTCTTTGTTCCTTTGTTGGATGGTTTCCTTGTCAACTATCAAGAATCCCAATCTGTGAT
TCATAATTTGTTGGACCAGATTCCAGACATGTTTGCAGACTCTAATGAAAATGAGACTGTCTTTGCTCCT
GTCATCCAGGCTGGCATGGAAGCACTAAAGGCAGCAGACTGTCCTGGGAAGCTGTTCATCTTCCATTCTT
CCTTGCCAACTGCTGAAGCACCAGGGAAGCTCAAAAACAGAGATGACAAAAAACTGGTTAATACAGACAA
AGAGAAGATACTTTTCCAGCCCCAAACAAATGTCTATGACTCATTGGCCAAGGACTGCGTGGCTCACGGC
TGCTCTGTGACACTCTTCCTCTTTCCTAGTCAGTATGTGGACGTGGCCTCGCTGGGGCTGGTTCCTCAGC
TCACTGGAGGAACCCTTTACAAATACAACAATTTCCAGATGCACTTGGATAGACAACAATTTTTGAACGA
CCTCAGAAATGATATTGAAAAGAAAATAGGCTTTGATGCTATTATGAGGGTTCGTACCAGCACAGGTTTC
AGAGCCACTGATTTCTTTGGTGGAATCTTGATGAACAACACCACCGATGTAGAAATGGCTGCCATCGATT
GTGACAAGGCAGTGACCGTGGAGTTCAAGCACGATGACAAACTCAGTGAAGACAGTGGAGCCTTAATCCA
GTGTGCTGTGCTTTACACGACAATCAGTGGTCAAAGAAGACTTCGGATTCACAATCTTGGCTTAAACTGC
AGCTCTCAGCTAGCTGATCTTTATAAGAGCTGTGAGACAGATGCTCTTATCAACTTCTTTGCCAAGTCAG
CTTTTAAAGCAGTTCTTCACCAGCCTTTGAAGGTCATCCGGGAAATTCTAGTTAATCAGACTGCCCATAT
GTTGGCATGTTACCGGAAGAATTGTGCAAGTCCTTCTGCAGCAAGCCAGCTTATTCTACCAGATTCCATG
AAAGTATTGCCAGTGTACATGAATTGCTTGTTGAAAAACTGTGTACTACTCAGCAGACCAGAGATCTCAA
CTGATGAACGAGCATACCAGAGACAGCTGGTCATGACCATGGGTGTGGCTGACTCTCAGCTTTTCTTCTA
CCCACAACTTCTGCCCATACACACGTTAGATGTCAAGAGTACAATGTTACCTGCTGCCGTTCGTTGCTCT
GAGTCCCGTCTTTCAGAAGAAGGAATATTCTTACTGGCTAATGGTCTACACATGTTCCTGTGGTTGGGAG
TAAGCAGCCCACCAGAACTGATCCAAGGAATATTTAATGTGCCATCTTTTGCACATATCAACACAGATAT
GACATTGCTGCCTGAAGTGGGAAACCCATACTCTCAACAACTCAGAATGATAATGGGTATTATCCAACAA
AAGAGGCCATATTCAATGAAGCTCACAATTGTAAAGCAGCGAGAACAACCAGAAATGGTTTTCCGACAGT
TCCTGGTAGAAGACAAAGGACTTTACGGAGGCTCTTCTTATGTGGATTTCCTTTGTTGTGTTCACAAGGA
GATCTGTCAGCTGCTTAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207747 protein sequence
Red=Cloning site Green=Tags(s)

MSQQGYVATPPYSQPQPGIGLSPPHYGHYGDPSHTASPTGMMKPAGPLGATATRGMLPPGPPPPGPHQFG
QNGAHATGHPPQRFPGPPPVNNVASSHAPYQPSAQSSYPGPISTSSVTQLGSQLSAMQINSYGSGMAPPS
QGPPGPLSATSLQTPPRPPQPSILQPGSQVLPPPPTTLNGPGASPLPLPMYRPDGLSGPPPPNAQYQPPP
LPGQTLGAGYPPQQAANSGPQMAGAQLSYPGGFPGGPAQMAGPPQPQKKLDPDSIPSPIQVIENDRASRG
GQVYATNTRGQIPPLVTTDCMIQDQGNASPRFIRCTTYCFPCTSDMAKQAQIPLAAVIKPFATIPSNESP
LYLVNHGESGPVRCNRCKAYMCPFMQFIEGGRRYQCGFCNCVNDVPPFYFQHLDHIGRRLDHYEKPELSL
GSYEYVATLDYCRKSKPPNPPAFIFMIDVSYSNIKNGLVKLICEELKTMLEKIPKEEQEETSAIRVGFIT
YNKVLHFFNVKSNLAQPQMMVVTDVGEVFVPLLDGFLVNYQESQSVIHNLLDQIPDMFADSNENETVFAP
VIQAGMEALKAADCPGKLFIFHSSLPTAEAPGKLKNRDDKKLVNTDKEKILFQPQTNVYDSLAKDCVAHG
CSVTLFLFPSQYVDVASLGLVPQLTGGTLYKYNNFQMHLDRQQFLNDLRNDIEKKIGFDAIMRVRTSTGF
RATDFFGGILMNNTTDVEMAAIDCDKAVTVEFKHDDKLSEDSGALIQCAVLYTTISGQRRLRIHNLGLNC
SSQLADLYKSCETDALINFFAKSAFKAVLHQPLKVIREILVNQTAHMLACYRKNCASPSAASQLILPDSM
KVLPVYMNCLLKNCVLLSRPEISTDERAYQRQLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCS
ESRLSEEGIFLLANGLHMFLWLGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQ
KRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014822
ORF Size 3099 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014822.4
RefSeq Size 4077 bp
RefSeq ORF 3099 bp
Locus ID 9871
UniProt ID O94855
Cytogenetics 4q26
Domains Gelsolin, Sec23_helical, Sec23_trunk, zf-Sec23_Sec24
MW 113.1 kDa
Summary The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. This gene product is implicated in the shaping of the vesicle, and also in cargo selection and concentration. Mutations in this gene have been associated with Cole-Carpenter syndrome, a disorder affecting bone formation, resulting in craniofacial malformations and bones that break easily. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Dec 2015
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Documents
Resources
"NM_014822" in other vectors (7)
SKU Description Size Price
RC207747L1 Lenti ORF clone of Human SEC24 family, member D (S. cerevisiae) (SEC24D), Myc-DDK-tagged 10 ug
$1,199.00
RC207747L2 Lenti ORF clone of Human SEC24 family, member D (S. cerevisiae) (SEC24D), mGFP tagged 10 ug
$1,199.00
RC207747L3 Lenti ORF clone of Human SEC24 family, member D (S. cerevisiae) (SEC24D), Myc-DDK-tagged 10 ug
$1,199.00
RC207747L4 Lenti ORF clone of Human SEC24 family, member D (S. cerevisiae) (SEC24D), mGFP tagged 10 ug
$1,199.00
RG207747 SEC24D (tGFP-tagged) - Human SEC24 family, member D (S. cerevisiae) (SEC24D) 10 ug
$789.00 $1,099.00
SC114788 SEC24D (untagged)-Human SEC24 family, member D (S. cerevisiae) (SEC24D) 10 ug
$899.00
SC324093 SEC24D (untagged)-Human SEC24 family, member D (S. cerevisiae) (SEC24D) 10 ug
$899.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.