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MBD1 (NM_015844) Human Tagged ORF Clone

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SKU
RC207708
MBD1 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $511.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MBD1
Synonyms CXXC3; PCM1; RFT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207708 representing NM_015844
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAGT
CAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAGT
TGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTGTGC
TATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCAGCCA
AGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAGACCAA
GGCTGACACTGACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTC
TCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGAATTGCCT
TCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTAACAGAAGA
CTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGTTCTGCAAGTGT
GAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAGTATGCCGGGGCTGTCAGACCC
AAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGCCAGTGGAAATG
TGTCCAGCGACGTTGCCTACGGGGTAAACATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGGCTGCC
AGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCCACAGAGCCGC
ACCCCAGAGCCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGTGTAGACGAGGACGAGCTAAA
GCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCGCCCCCACCTTACCGTCGTCGA
AAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACCTTGAAGCCCACCTTGGCTACACGCACAG
CCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAGCAGGTGGTGGCTTTGTGCTGCCCCCGCCTGG
CACTGACCTTGTGTTTTTACGGGAAGGCGCAAGCAGTCCTGTGCAGGTGCCGGGCCCTGTTGCAGCTTCC
ACAGAAGCCCTGTTGCAGGAGGCCCAGTGCTCTGGCCTGAGTTGGGTTGTGGCCTTACCCCAGGTGAAGC
AAGAGAAGGCGGATACCCAGGACGAGTGGACACCAGGCACAGCTGTCCTGACTTCTCCCGTATTGGTGCC
TGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCTTCTGTGAAGCAAGAGCCACCTGACCCAGAGGAG
GACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCCCCAGAGGAAGAGGCAGGAGGGGCTGGCA
CACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCTTCCGAGATACAGCAGTCTGGTTGCCAAG
GTCCAAAGACCTTAAAAAACCTGGAGCTAGAAAGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207708 representing NM_015844
Red=Cloning site Green=Tags(s)

MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGILC
YPAPKAHPVAVASKKRKKPSRPAKTRKRQVGPQSGEVRKEAPRDETKADTDTAPASFPAPGCCENCGISF
SGDGTQRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECAACQVTEDCGACSTCLLQLPHDVASGLFCKC
ERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLRPPRPGLRRQWKCVQRRCLRGKHARRKGGCDSKMAA
RRRPGAQPLPPPPPSQSPEPTEPHPRALAPSPPAEFIYYCVDEDELKRLLPSVWSESEDGAGSPPPYRRR
KRPSSARRHHLGPTLKPTLATRTAQPDHTQAPTKQEAGGGFVLPPPGTDLVFLREGASSPVQVPGPVAAS
TEALLQEAQCSGLSWVVALPQVKQEKADTQDEWTPGTAVLTSPVLVPGCPSKAVDPGLPSVKQEPPDPEE
DKEENKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKPGARKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015844
ORF Size 1647 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015844.2
RefSeq Size 2793 bp
RefSeq ORF 1650 bp
Locus ID 4152
UniProt ID Q9UIS9
Cytogenetics 18q21.1
Protein Families Druggable Genome, Transcription Factors
MW 59.8 kDa
Summary The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.provided by RefSeq, Feb 2011
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"NM_015844" in other vectors (6)
SKU Description Size Price
RC207708L1 Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, Myc-DDK-tagged 10 ug
$811.00
RC207708L2 Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, mGFP tagged 10 ug
$811.00
RC207708L3 Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, Myc-DDK-tagged 10 ug
$811.00
RC207708L4 Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, mGFP tagged 10 ug
$811.00
RG207708 MBD1 (tGFP-tagged) - Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3 10 ug
$711.00
SC124480 MBD1 (untagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3 10 ug
$512.00

Plasmid Map for RC207708

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207708
Circular map for RC207708

file-alt Citations

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