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PPAN (NM_020230) Human Tagged ORF Clone

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SKU
RC207702
PPAN (Myc-DDK-tagged)-Human peter pan homolog (Drosophila) (PPAN)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPAN
Synonyms BXDC3; SSF; SSF-1; SSF1; SSF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207702 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACAGTCAGGGAGGTCCCGGCACCAGAAGCGCGCCCGCGCCCAGGCGCAGCTCCGCAACCTCGAGG
CCTATGCCGCGAACCCGCACTCGTTCGTGTTCACGCGAGGCTGCACGGGTCGCAACATCCGGCAGCTCAG
CCTGGACGTGCGGCGGGTCATGGAGCCGCTCACTGCCAGCCGTCTGCAGGTTCGTAAGAAGAACTCGCTG
AAGGACTGCGTGGCAGTGGCTGGGCCCCTCGGGGTCACACACTTTCTGATCCTGAGCAAAACAGAGACCA
ATGTCTACTTTAAGCTGATGCGCCTCCCAGGAGGCCCCACCTTGACCTTCCAGGTCAAGAAGTACTCGCT
GGTGCGTGATGTGGTCTCCTCACTGCGCCGGCACCGCATGCACGAGCAGCAGTTTGCCCACCCACCCCTC
CTGGTACTCAACAGCTTTGGCCCCCATGGTATGCATGTGAAGCTCATGGCCACCATGTTCCAGAACCTGT
TCCCCTCCATCAACGTGCACAAGGTGAACCTGAACACCATCAAGCGCTGCCTCCTCATCGACTACAACCC
CGACTCCCAGGAGCTGGACTTCCGCCACTATAGCATCAAAGTTGTTCCTGTGGGCGCGAGTCGCGGGATG
AAGAAGCTGCTCCAGGAGAAGTTCCCCAACATGAGCCGCCTGCAGGACATCAGCGAGCTGCTGGCCACGG
GCGCGGGGCTGTCGGAGAGCGAGGCAGAGCCTGACGGCGACCACAACATCACAGAGCTGCCTCAGGCTGT
CGCTGGCCGTGGCAACATGCGGGCCCAGCAGAGTGCAGTGCGGCTCACCGAGATCGGCCCGCGGATGACA
CTGCAGCTCATCAAGGTCCAGGAGGGCGTCGGGGAGGGCAAAGTGATGTTCCACAGTTTTGTGAGCAAGA
CGGAGGAGGAGCTGCAGGCCATCCTGGAAGCCAAGGAGAAGAAGCTGCGGCTGAAGGCGCAGAGGCAGGC
CCAGCAGGCCCAGAATGTGCAGCGCAAGCAGGAGCAGCGGGAGGCCCACAGAAAGAAGAGCCTGGAGGGC
ATGAAGAAGGCACGGGTCGGGGGTAGTGATGAAGAGGCCTCTGGGATCCCTTCAAGGACGGCGAGCCTGG
AGTTGGGTGAGGACGATGATGAACAGGAAGATGATGACATCGAGTATTTCTGCCAGGCGGTGGGCGAGGC
GCCCAGTGAGGACCTGTTCCCCGAGGCCAAGCAGAAACGGCTTGCCAAGTCTCCAGGGCGGAAGCGGAAG
CGGTGGGAAATGGATCGAGGCAGGGGTCGCCTTTGTGACCAGAAGTTTCCCAAGACCAAGGACAAGTCCC
AGGGAGCCCAGGCCAGGCGGGGGCCCAGAGGGGCTTCCCGGGATGGTGGGCGAGGCCGGGGCCGGGGCCG
CCCAGGGAAGAGAGTGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207702 protein sequence
Red=Cloning site Green=Tags(s)

MGQSGRSRHQKRARAQAQLRNLEAYAANPHSFVFTRGCTGRNIRQLSLDVRRVMEPLTASRLQVRKKNSL
KDCVAVAGPLGVTHFLILSKTETNVYFKLMRLPGGPTLTFQVKKYSLVRDVVSSLRRHRMHEQQFAHPPL
LVLNSFGPHGMHVKLMATMFQNLFPSINVHKVNLNTIKRCLLIDYNPDSQELDFRHYSIKVVPVGASRGM
KKLLQEKFPNMSRLQDISELLATGAGLSESEAEPDGDHNITELPQAVAGRGNMRAQQSAVRLTEIGPRMT
LQLIKVQEGVGEGKVMFHSFVSKTEEELQAILEAKEKKLRLKAQRQAQQAQNVQRKQEQREAHRKKSLEG
MKKARVGGSDEEASGIPSRTASLELGEDDDEQEDDDIEYFCQAVGEAPSEDLFPEAKQKRLAKSPGRKRK
RWEMDRGRGRLCDQKFPKTKDKSQGAQARRGPRGASRDGGRGRGRGRPGKRVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020230
ORF Size 1419 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020230.7
RefSeq Size 1745 bp
RefSeq ORF 1422 bp
Locus ID 56342
UniProt ID Q9NQ55
Cytogenetics 19p13.2
Domains Brix
Protein Families Druggable Genome, Stem cell - Pluripotency
MW 53.2 kDa
Summary The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation. provided by RefSeq, Nov 2010
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Documents
Resources
"NM_020230" in other vectors (6)
SKU Description Size Price
RC207702L1 Lenti ORF clone of Human peter pan homolog (Drosophila) (PPAN), Myc-DDK-tagged 10 ug
$757.00
RC207702L2 Lenti ORF clone of Human peter pan homolog (Drosophila) (PPAN), mGFP tagged 10 ug
$757.00
RC207702L3 Lenti ORF clone of Human peter pan homolog (Drosophila) (PPAN), Myc-DDK-tagged 10 ug
$757.00
RC207702L4 Lenti ORF clone of Human peter pan homolog (Drosophila) (PPAN), mGFP tagged 10 ug
$757.00
RG207702 PPAN (tGFP-tagged) - Human peter pan homolog (Drosophila) (PPAN) 10 ug
$489.00 $657.00
SC113147 PPAN (untagged)-Human peter pan homolog (Drosophila) (PPAN) 10 ug
$457.00

Plasmid Map for RC207702

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207702
Circular map for RC207702

file-alt Citations

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