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TFIP11 (NM_012143) Human Tagged ORF Clone

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SKU
RC207682
TFIP11 (Myc-DDK-tagged)-Human tuftelin interacting protein 11 (TFIP11), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $766.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TFIP11
Synonyms bK445C9.6; NTR1; Spp382; STIP; STIP-1; TIP39
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207682 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCATTGTCCCACTTATACCGGGATGGGGAAGGCCGCATTGATGATGATGATGACGAGCGGGAGAACT
TTGAGATCACTGACTGGGATCTCCAGAATGAGTTCAACCCCAACCGACAGCGCCACTGGCAGACCAAGGA
AGAAGCCACCTACGGGGTGTGGGCAGAGCGAGACTCGGATGATGAGAGGCCCAGCTTTGGAGGCAAACGG
GCCCGTGACTACTCTGCGCCAGTCAACTTCATCAGCGCAGGGCTCAAGAAAGGGGCAGCGGAGGAGGCAG
AGTCGGAAGATTCTGATGACGAAGAGAAACCTGTTAAGCAGGACGACTTTCCTAAGGATTTTGGACCAAG
GAAGCTAAAAACGGGTGGCAATTTTAAGCCCAGCCAGAAAGGTTTTGCAGGAGGAACCAAATCTTTCATG
GACTTCGGCAGCTGGGAAAGACACACAAAAGGAATTGGACAGAAGCTTCTTCAGAAGATGGGCTACGTCC
CTGGACGGGGCCTCGGGAAGAATGCACAAGGTATCATTAACCCAATTGAAGCCAAGCAGAGAAAGGGAAA
AGGTGCTGTGGGGGCTTATGGATCCGAGCGCACCACTCAGTCCATGCAAGACTTCCCTGTGGTTGACTCA
GAGGAAGAAGCTGAAGAGGAGTTTCAGAAGGAGCTGAGCCAGTGGAGGAAAGACCCAAGTGGAAGCAAGA
AGAAGCCCAAATACTCTTACAAGACCGTGGAAGAGTTGAAGGCCAAGGGCAGGATTAGCAAGAAGCTCAC
TGCTCCCCAGAAGGAACTTTCTCAAGTCAAGGTCATAGACATGACAGGCCGGGAGCAGAAGGTCTACTAC
AGCTACAGTCAGATCAGCCACAAGCACAACGTTCCCGATGATGGGCTGCCGCTACAGTCCCAACAGCTGC
CACAGTCTGGCAAAGAGGCCAAGGCCCCCGGCTTCGCGCTGCCCGAGCTGGAGCACAACCTGCAGCTGCT
CATCGACCTCACGGAGCAGGAGATCATCCAGAATGACCGGCAGCTACAGTATGAGCGGGACATGGTGGTC
AACCTCTTCCATGAGCTGGAGAAGATGACCGAGGTCCTGGACCACGAGGAGCGGGTCATCTCGAACCTCA
GCAAGGTCCTGGAGATGGTGGAGGAGTGCGAGCGGCGGATGCAGCCCGACTGCAGCAACCCCCTCACCCT
GGACGAGTGTGCCCGCATCTTCGAAACCCTGCAGGACAAGTACTATGAGGAGTACAGGATGTCCGACCGT
GTGGACCTTGCTGTGGCCATCGTCTATCCACTCATGAAGGAGTACTTCAAGGAGTGGGATCCCCTCAAAG
ACTGCACTTATGGCACCGAGATCATCTCTAAGTGGAAAAGCCTCCTAGAGAATGACCAGCTCTTGTCCCA
TGGCGGACAGGACCTCTCAGCAGATGCCTTTCACAGGTTGATATGGGAAGTCTGGATGCCTTTTGTTCGA
AATATTGTCACCCAGTGGCAGCCAAGGAACTGTGACCCGATGGTGGACTTTTTGGATAGTTGGGTGCACA
TTATTCCTGTGTGGATCTTAGATAACATACTGGACCAACTCATCTTCCCCAAGCTGCAAAAGGAGGTGGA
AAACTGGAACCCGCTCACAGACACTGTTCCCATCCACTCTTGGATCCACCCATGGCTGCCCCTTATGCAG
GCACGGCTGGAGCCACTCTATTCCCCCATCCGTAGTAAGCTGTCCAGCGCCCTGCAGAAGTGGCACCCCA
GCGACTCCTCTGCCAAGCTCATCCTCCAGCCCTGGAAGGATGTCTTCACTCCTGGCTCCTGGGAAGCATT
CATGGTCAAAAACATAGTGCCCAAGCTGGGGATGTGTCTTGGTGAGCTAGTCATTAACCCCCACCAGCAG
CACATGGATGCATTCTATTGGGTGATTGACTGGGAAGGGATGATCTCTGTCTCTAGCCTGGTGGGACTTC
TTGAAAAGCACTTCTTCCCCAAGTGGCTTCAGGTGCTGTGCTCTTGGCTCAGTAACAGCCCAAATTATGA
GGAGATCACCAAGTGGTACCTGGGTTGGAAGTCGATGTTCTCAGACCAAGTGCTGGCACATCCATCTGTC
AAGGACAAATTTAATGAGGCACTTGATATCATGAACCGGGCGGTGTCCTCCAACGTTGGTGCCTACATGC
AGCCAGGAGCACGGGAGAACATTGCCTATCTCACCCACACGGAGCGGAGGAAGGACTTCCAGTACGAGGC
CATGCAGGAGAGGCGGGAGGCTGAGAACATGGCTCAGAGGGGCATTGGCGTGGCCGCTAGCTCTGTGCCC
ATGAACTTTAAGGACCTCATTGAGACCAAGGCTGAGGAGCACAACATTGTCTTCATGCCCGTCATTGGGA
AGCGACACGAAGGGAAGCAGCTCTACACCTTTGGCCGCATTGTGATCTACATCGACCGGGGAGTGGTCTT
TGTCCAGGGCGAGAAGACGTGGGTGCCCACCTCACTGCAGAGCCTGATCGACATGGCCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207682 protein sequence
Red=Cloning site Green=Tags(s)

MSLSHLYRDGEGRIDDDDDERENFEITDWDLQNEFNPNRQRHWQTKEEATYGVWAERDSDDERPSFGGKR
ARDYSAPVNFISAGLKKGAAEEAESEDSDDEEKPVKQDDFPKDFGPRKLKTGGNFKPSQKGFAGGTKSFM
DFGSWERHTKGIGQKLLQKMGYVPGRGLGKNAQGIINPIEAKQRKGKGAVGAYGSERTTQSMQDFPVVDS
EEEAEEEFQKELSQWRKDPSGSKKKPKYSYKTVEELKAKGRISKKLTAPQKELSQVKVIDMTGREQKVYY
SYSQISHKHNVPDDGLPLQSQQLPQSGKEAKAPGFALPELEHNLQLLIDLTEQEIIQNDRQLQYERDMVV
NLFHELEKMTEVLDHEERVISNLSKVLEMVEECERRMQPDCSNPLTLDECARIFETLQDKYYEEYRMSDR
VDLAVAIVYPLMKEYFKEWDPLKDCTYGTEIISKWKSLLENDQLLSHGGQDLSADAFHRLIWEVWMPFVR
NIVTQWQPRNCDPMVDFLDSWVHIIPVWILDNILDQLIFPKLQKEVENWNPLTDTVPIHSWIHPWLPLMQ
ARLEPLYSPIRSKLSSALQKWHPSDSSAKLILQPWKDVFTPGSWEAFMVKNIVPKLGMCLGELVINPHQQ
HMDAFYWVIDWEGMISVSSLVGLLEKHFFPKWLQVLCSWLSNSPNYEEITKWYLGWKSMFSDQVLAHPSV
KDKFNEALDIMNRAVSSNVGAYMQPGARENIAYLTHTERRKDFQYEAMQERREAENMAQRGIGVAASSVP
MNFKDLIETKAEEHNIVFMPVIGKRHEGKQLYTFGRIVIYIDRGVVFVQGEKTWVPTSLQSLIDMAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012143
ORF Size 2511 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012143.4
RefSeq Size 2864 bp
RefSeq ORF 2514 bp
Locus ID 24144
UniProt ID Q9UBB9, A0A024R1I7
Cytogenetics 22q12.1
Domains G-patch
MW 96.8 kDa
Summary This gene encodes a protein component of the spliceosome that promotes the release of the lariat-intron during late-stage splicing through the recruitment of a pre-mRNA splicing factor called DEAH-box helicase 15. The encoded protein contains a G-patch domain, a hallmark of RNA-processing proteins, that binds DEAH-box helicase 15. This protein contains an atypical nuclear localization sequence as well as a nuclear speckle-targeting sequence, enabling it to localize to distinct speckled regions within the cell nucleus. Polymorphisms in this gene are associated with dental caries suggesting a role in amelogenesis. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2016
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"NM_012143" in other vectors (7)
SKU Description Size Price
RC207682L1 Lenti ORF clone of Human tuftelin interacting protein 11 (TFIP11), transcript variant 2, Myc-DDK-tagged 10 ug
$1,066.00
RC207682L2 Lenti ORF clone of Human tuftelin interacting protein 11 (TFIP11), transcript variant 2, mGFP tagged 10 ug
$1,066.00
RC207682L3 Lenti ORF clone of Human tuftelin interacting protein 11 (TFIP11), transcript variant 2, Myc-DDK-tagged 10 ug
$1,066.00
RC207682L4 Lenti ORF clone of Human tuftelin interacting protein 11 (TFIP11), transcript variant 2, mGFP tagged 10 ug
$1,066.00
RG207682 TFIP11 (tGFP-tagged) - Human tuftelin interacting protein 11 (TFIP11), transcript variant 2 10 ug
$789.00 $966.00
SC112654 TFIP11 (untagged)-Human tuftelin interacting protein 11 (TFIP11), transcript variant 2 10 ug
$767.00
SC320803 TFIP11 (untagged)-Human tuftelin interacting protein 11 (TFIP11), transcript variant 2 10 ug
$767.00

Plasmid Map for RC207682

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207682
Circular map for RC207682

file-alt Citations

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