DOK2 (NM_003974) Human Tagged ORF Clone

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SKU
RC207621
DOK2 (Myc-DDK-tagged)-Human docking protein 2, 56kDa (DOK2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DOK2
Synonyms p56DOK; p56dok-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207621 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGACGGGGCAGTGAAACAAGGCTTCTTGTATCTTCAGCAGCAGCAGACGTTTGGAAAGAAATGGC
GCCGCTTCGGCGCCTCACTGTATGGAGGGTCGGACTGCGCCTTGGCCCGGCTGGAGCTGCAGGAGGGCCC
GGAGAAGCCTCGTCGGTGTGAGGCTGCCCGGAAGGTCATCCGCCTCAGTGACTGCCTGCGGGTGGCCGAG
GCCGGCGGAGAGGCCAGCAGCCCCCGGGACACCAGTGCCTTCTTCCTGGAGACCAAGGAGCGCCTGTACC
TCCTGGCGGCCCCTGCAGCGGAGCGCGGCGACTGGGTGCAGGCCATCTGCCTCCTGGCCTTCCCCGGGCA
GAGGAAGGAGCTCTCGGGGCCAGAGGGAAAGCAGAGCCGGCCCTGCATGGAGGAAAATGAATTGTACAGC
AGCGCAGTCACAGTCGGCCCCCACAAGGAATTTGCTGTGACCATGAGACCTACAGAAGCCAGTGAGAGGT
GCCACCTGCGGGGGTCCTATACCCTCCGGGCTGGGGAGAGTGCCCTGGAGCTGTGGGGTGGGCCCGAGCC
AGGGACCCAGCTGTACGACTGGCCCTACAGGTTTCTGCGGCGCTTTGGGCGGGACAAGGTAACCTTTTCC
TTTGAGGCAGGCCGTCGCTGCGTCTCTGGAGAGGGCAACTTTGAGTTCGAAACCCGGCAAGGCAATGAGA
TCTTCTTGGCCCTGGAAGAGGCCATCTCTGCCCAGAAGAATGCTGCACCCGCTACACCCCAACCGCAGCC
AGCCACAATCCCCGCCTCGCTGCCCCGGCCTGATAGCCCCTACTCTCGGCCGCATGACTCACTGCCGCCG
CCTTCACCCACCACACCGGTGCCTGCTCCACGGCCTCGGGGCCAGGAGGGGGAGTATGCCGTGCCCTTCG
ATGCGGTGGCCCGTTCCTTGGGGAAGAACTTCAGGGGCATCTTGGCAGTCCCTCCTCAGCTCCTGGCCGA
CCCTCTGTACGACAGCATTGAGGAGACCCTGCCCCCTCGACCTGACCACATATACGATGAGCCCGAGGGA
GTGGCTGCCCTGTCCCTCTATGACAGCCCGCAGGAGCCCCGGGGTGAGGCATGGAGGAGGCAGGCGACAG
CTGACAGGGACCCTGCTGGCCTCCAGCATGTCCAGCCAGCTGGGCAGGATTTCTCTGCTTCTGGCTGGCA
GCCAGGAACTGAGTATGACAATGTTGTACTAAAGAAAGGCCCAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207621 protein sequence
Red=Cloning site Green=Tags(s)

MGDGAVKQGFLYLQQQQTFGKKWRRFGASLYGGSDCALARLELQEGPEKPRRCEAARKVIRLSDCLRVAE
AGGEASSPRDTSAFFLETKERLYLLAAPAAERGDWVQAICLLAFPGQRKELSGPEGKQSRPCMEENELYS
SAVTVGPHKEFAVTMRPTEASERCHLRGSYTLRAGESALELWGGPEPGTQLYDWPYRFLRRFGRDKVTFS
FEAGRRCVSGEGNFEFETRQGNEIFLALEEAISAQKNAAPATPQPQPATIPASLPRPDSPYSRPHDSLPP
PSPTTPVPAPRPRGQEGEYAVPFDAVARSLGKNFRGILAVPPQLLADPLYDSIEETLPPRPDHIYDEPEG
VAALSLYDSPQEPRGEAWRRQATADRDPAGLQHVQPAGQDFSASGWQPGTEYDNVVLKKGPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003974
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003974.4
RefSeq Size 1870 bp
RefSeq ORF 1239 bp
Locus ID 9046
UniProt ID O60496
Cytogenetics 8p21.3
Domains IRS, PH
Protein Families Druggable Genome
MW 45.4 kDa
Summary The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_003974" in other vectors (6)
SKU Description Size Price
RC207621L1 Lenti ORF clone of Human docking protein 2, 56kDa (DOK2), Myc-DDK-tagged 10 ug
$757.00
RC207621L2 Lenti ORF clone of Human docking protein 2, 56kDa (DOK2), mGFP tagged 10 ug
$757.00
RC207621L3 Lenti ORF clone of Human docking protein 2, 56kDa (DOK2), Myc-DDK-tagged 10 ug
$757.00
RC207621L4 Lenti ORF clone of Human docking protein 2, 56kDa (DOK2), mGFP tagged 10 ug
$757.00
RG207621 DOK2 (tGFP-tagged) - Human docking protein 2, 56kDa (DOK2) 10 ug
$489.00 $657.00
SC117635 DOK2 (untagged)-Human docking protein 2, 56kDa (DOK2) 10 ug
$457.00

Plasmid Map for RC207621

Download sequence for

Cloning scheme for RC207621

Circular map for RC207621

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