Spasmolytic Polypeptide (TFF2) (NM_005423) Human Tagged ORF Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Spasmolytic Polypeptide |
| Synonyms | SML1; SP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
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| ACCN | NM_005423 |
| ORF Size | 387 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005423.5 |
| RefSeq Size | 717 bp |
| RefSeq ORF | 390 bp |
| Locus ID | 7032 |
| UniProt ID | Q03403 |
| Cytogenetics | 21q22.3 |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 14.3 kDa |
| Summary | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005423" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207571L1 | Lenti ORF clone of Human trefoil factor 2 (TFF2), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC207571L2 | Lenti ORF clone of Human trefoil factor 2 (TFF2), mGFP tagged | 10 ug |
$450.00
|
|
| RC207571L3 | Lenti ORF clone of Human trefoil factor 2 (TFF2), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC207571L4 | Lenti ORF clone of Human trefoil factor 2 (TFF2), mGFP tagged | 10 ug |
$450.00
|
|
| RG207571 | TFF2 (tGFP-tagged) - Human trefoil factor 2 (TFF2) | 10 ug |
$489.00
|
|
| SC323966 | TFF2 (untagged)-Human trefoil factor 2 (TFF2) | 10 ug |
$150.00
|
|
Plasmid Map for RC207571
Download sequence for
Cloning scheme for RC207571
Circular map for RC207571
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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