TESK1 (NM_006285) Human Tagged ORF Clone

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SKU
RC207560
TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1)
  $583.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TESK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207560 representing NM_006285
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGGAACGGCCCCCACTGCGGGGCCCTGGGCCCGGGCCTGGAGAGGTGCCGGGGGAGGGGCCCC
CGGGGCCGGGGGGCACGGGCGGAGGCCCGGGCCGGGGCCGCCCCTCCTCCTACCGGGCTCTCCGCAGCGC
CGTGTCTAGCCTGGCGCGTGTGGACGATTTTCACTGCGCGGAGAAGATCGGGGCCGGCTTCTTCTCTGAG
GTCTACAAGGTTCGGCACCGACAGTCAGGGCAAGTCATGGTGCTGAAGATGAACAAGCTCCCCAGTAACC
GGGGCAACACACTACGGGAAGTGCAGCTGATGAACCGGCTCAGGCACCCCAACATCCTAAGGTTCATGGG
AGTCTGTGTGCACCAGGGACAGCTGCACGCTCTTACAGAGTATATGAATGGGGGGACATTGGAACAGCTG
CTCAGCTCCCCTGAACCCCTATCCTGGCCGGTCAGGCTCCACCTGGCCCTGGACATTGCCCGAGGCCTGC
GGTACCTGCACTCCAAAGGTGTATTTCACCGCGACCTCACATCCAAGAACTGTCTAGTCCGACGGGAAGA
TCGAGGCTTCACCGCTGTCGTGGGTGACTTCGGGCTGGCCGAAAAGATTCCTGTGTATAGGGAGGGGGCA
AGGAAGGAGCCATTGGCCGTGGTGGGCTCCCCATACTGGATGGCTCCAGAGGTGTTACGGGGTGAGCTGT
ATGATGAGAAGGCTGATGTCTTTGCCTTCGGGATTGTCCTCTGTGAGCTCATCGCCCGAGTACCTGCAGA
CCCTGACTACCTACCACGCACTGAGGACTTTGGCCTGGATGTGCCTGCTTTCCGAACTCTGGTGGGGGAT
GACTGCCCACTGCCTTTCTTGCTCCTGGCCATCCACTGCTGCAACCTGGAACCCAGCACCCGTGCCCCCT
TCACCGAAATTACCCAGCACCTGGAATGGATCCTGGAGCAGCTGCCTGAGCCAGCCCCACTCACCAGGAC
CGCCCTGACACACAATCAGGGCTCTGTTGCAAGAGGGGGTCCCTCTGCCACGCTTCCCAGGCCAGATCCC
CGGCTTTCCCGAAGCCGGTCAGACCTCTTCCTGCCCCCATCACCAGAATCACCCCCCAACTGGGGGGACA
ATCTGACTCGAGTCAACCCCTTCTCACTACGGGAAGACCTCAGGGGTGGCAAGATCAAGCTCTTAGACAC
ACCCAGCAAGCCAGTCCTGCCTCTTGTGCCACCATCACCATTCCCATCCACTCAGCTGCCCTTGGTGACC
ACTCCGGAGACCCTGGTCCAGCCTGGGACACCTGCCCGCCGCTGCCGCTCACTACCCTCATCCCCCGAGC
TCCCCCGCCGTATGGAGACAGCACTGCCAGGTCCTGGCCCTCCCGCTGTGGGCCCCTCGGCTGAAGAGAA
GATGGAGTGCGAGGGCAGCAGCCCTGAGCCGGAACCTCCAGGGCCAGCGCCCCAGCTGCCTCTGGCTGTG
GCCACAGACAACTTCATCAGCACCTGTTCCTCGGCCTCCCAACCCTGGTCCCCTAGATCAGGACCCGTCC
TCAATAACAATCCCCCAGCTGTGGTGGTGAACTCCCCACAAGGCTGGGCTGGGGAGCCCTGGAACCGGGC
CCAGCATAGCCTGCCCCGGGCGGCAGCCCTGGAGCGGACAGAACCCTCGCCACCCCCTTCAGCTCCCCGG
GAGCCCGATGAGGGGCTGCCCTGTCCTGGCTGCTGCCTCGGCCCCTTCAGCTTTGGCTTCCTGTCCATGT
GCCCCCGCCCCACACCAGCTGTTGCCCGCTACCGCAACCTGAACTGTGAGGCGGGCAGTCTCCTCTGCCA
CCGAGGGCACCACGCCAAGCCACCCACACCCAGCCTGCAGCTGCCTGGGGCACGCTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207560 representing NM_006285
Red=Cloning site Green=Tags(s)

MAGERPPLRGPGPGPGEVPGEGPPGPGGTGGGPGRGRPSSYRALRSAVSSLARVDDFHCAEKIGAGFFSE
VYKVRHRQSGQVMVLKMNKLPSNRGNTLREVQLMNRLRHPNILRFMGVCVHQGQLHALTEYMNGGTLEQL
LSSPEPLSWPVRLHLALDIARGLRYLHSKGVFHRDLTSKNCLVRREDRGFTAVVGDFGLAEKIPVYREGA
RKEPLAVVGSPYWMAPEVLRGELYDEKADVFAFGIVLCELIARVPADPDYLPRTEDFGLDVPAFRTLVGD
DCPLPFLLLAIHCCNLEPSTRAPFTEITQHLEWILEQLPEPAPLTRTALTHNQGSVARGGPSATLPRPDP
RLSRSRSDLFLPPSPESPPNWGDNLTRVNPFSLREDLRGGKIKLLDTPSKPVLPLVPPSPFPSTQLPLVT
TPETLVQPGTPARRCRSLPSSPELPRRMETALPGPGPPAVGPSAEEKMECEGSSPEPEPPGPAPQLPLAV
ATDNFISTCSSASQPWSPRSGPVLNNNPPAVVVNSPQGWAGEPWNRAQHSLPRAAALERTEPSPPPSAPR
EPDEGLPCPGCCLGPFSFGFLSMCPRPTPAVARYRNLNCEAGSLLCHRGHHAKPPTPSLQLPGARS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006285
ORF Size 1878 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006285.3
RefSeq Size 2559 bp
RefSeq ORF 1881 bp
Locus ID 7016
UniProt ID Q15569, Q8NFJ4
Cytogenetics 9p13.3
Protein Families Druggable Genome, Protein Kinase
MW 67.5 kDa
Summary This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain and a C-terminal proline-rich domain. Its protein kinase domain is most closely related to those of the LIM motif-containing protein kinases (LIMKs). The encoded protein can phosphorylate myelin basic protein and histone in vitro. The testicular germ cell-specific expression and developmental pattern of expression of the mouse gene suggests that this gene plays an important role at and after the meiotic phase of spermatogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Dec 2015
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Documents
Resources
"NM_006285" in other vectors (8)
SKU Description Size Price
RC207560L1 Lenti-ORF clone of TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RC207560L2 Lenti-ORF clone of TESK1 (mGFP-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RC207560L3 Lenti-ORF clone of TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RC207560L4 Lenti-ORF clone of TESK1 (mGFP-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RG207560 TESK1 (tGFP-tagged) - Human testis-specific kinase 1 (TESK1) 10 ug
$783.00
SC116192 TESK1 (untagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$584.00
SC123458 TESK1 (untagged)-Human testis-specific kinase 1 (cDNA clone MGC:45208 IMAGE:5170888), complete cds 10 ug
$781.00
SC323413 TESK1 (untagged)-Kinase deficient mutant (K86M) of Human testis-specific kinase 1 (TESK1) 10 ug
$584.00

Plasmid Map for RC207560

Download sequence for

Cloning scheme for RC207560

Circular map for RC207560

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