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TESK1 (NM_006285) Human Untagged Clone

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SKU
SC323413
TESK1 (untagged)-Kinase deficient mutant (K86M) of Human testis-specific kinase 1 (TESK1)
  $584.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TESK1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_006285, the custom clone sequence may differ by one or more nucleotides


ATGGCCGGGGAACGGCCCCCACTGCGGGGCCCTGGGCCCGGGCCTGGAGAGGTGCCGGGGGAGGGGCCCC
CGGGGCCGGGGGGCACGGGCGGAGGCCCGGGCCGGGGCCGCCCCTCCTCCTACCGGGCTCTCCGCAGCGC
CGTGTCTAGCCTGGCGCGTGTGGACGATTTTCACTGCGCGGAGAAGATCGGGGCCGGCTTCTTCTCTGAG
GTCTACAAGGTTCGGCACCGACAGTCAGGGCAAGTCATGGTGCTGAAGATGAACAAGCTCCCCAGTAACC
GGGGCAACACACTACGGGAAGTGCAGCTGATGAACCGGCTCAGGCACCCCAACATCCTAAGGTTCATGGG
AGTCTGTGTGCACCAGGGACAGCTGCACGCTCTTACAGAGTATATGAATGGGGGGACATTGGAACAGCTG
CTCAGCTCCCCTGAACCCCTGTCCTGGCCGGTCAGGCTCCACCTGGCCCTGGACATTGCCCGAGGCCTGC
GGTACCTGCACTCCAAAGGTGTATTTCACCGCGACCTCACATCCAAGAACTGTCTAGTCCGACGGGAAGA
TCGAGGCTTCACCGCTGTCGTGGGTGACTTCGGGCTGGCCGAAAAGATTCCTGTGTATAGGGAGGGGGCA
AGGAAGGAGCCATTGGCCGTGGTGGGCTCCCCATACTGGATGGCTCCAGAGGTGTTACGGGGTGAGCTGT
ATGATGAGAAGGCTGATGTCTTTGCCTTCGGGATTGTCCTCTGTGAGCTCATCGCCCGAGTACCTGCAGA
CCCTGACTACCTACCACGCACTGAGGACTTTGGCCTGGATGTGCCTGCTTTCCGAACTCTGGTGGGGGAT
GACTGCCCACTGCCTTTCTTGCTCCTGGCCATCCACTGCTGCAACCTGGAACCCAGCACCCGTGCCCCCT
TCACCGAAATTACCCAGCACCTGGAATGGATCCTGGAGCAGCTGCCTGAGCCAGCCCCACTCACCAGGAC
CGCCCTGACACACAATCAGGGCTCTGTTGCAAGAGGGGGTCCCTCTGCCACGCTTCCCAGGCCAGATCCC
CGGCTTTCCCGAAGCCGGTCAGACCTCTTCCTGCCCCCATCACCAGAATCACCCCCCAACTGGGGGGACA
ATCTGACTCGAGTCAACCCCTTCTCACTACGGGAAGACCTCAGGGGTGGCAAGATCAAGCTCTTAGACAC
ACCCAGCAAGCCAGTCCTGCCTCTTGTGCCACCATCACCATTCCCATCCACTCAGCTGCCCTTGGTGACC
ACTCCGGAGACCCTGGTCCAGCCTGGGACACCTGCCCGCCGCTGCCGCTCACTACCCTCATCCCCCGAGC
TCCCCCGCCGTATGGAGACAGCACTGCCAGGTCCTGGCCCTCCCGCTGTGGGCCCCTCGGCTGAAGAGAA
GATGGAGTGCGAGGGCAGCAGCCCTGAGCCGGAACCTCCAGGGCCAGCGCCCCAGCTGCCTCTGGCTGTG
GCCACAGACAACTTCATCAGCACCTGTTCCTCGGCCTCCCAACCCTGGTCCCCTAGATCAGGACCCGTCC
TCAATAACAATCCCCCAGCTGTGGTGGTGAACTCCCCACAAGGCTGGGCTGGGGAGCCCTGGAACCGGGC
CCAGCATAGCCTGCCCCGGGCGGCAGCCCTGGAGCGGACAGAACCCTCGCCACCCCCTTCAGCTCCCCGG
GAGCCCGATGAGGGGCTGCCCTGTCCTGGCTGCTGCCTCGGCCCCTTCAGCTTTGGCTTCCTGTCCATGT
GCCCCCGCCCCACACCAGCTGTTGCCCGCTACCGCAACCTGAACTGTGAGGCGGGCAGTCTCCTCTGCCA
CCGAGGGCACCACGCCAAGCCACCCACACCCAGCCTGCAGCTGCCTGGGGCACGCTCTTAG

5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_006285 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCCGCGGCAAGCCA
GCCCGCCGGCGCCCCGGAGCGCTGCCGCCACCGTCACCGCCACCGCCCGGTCCAGGCCCAAGCCCAGGCC
CTGCGCGGAGCGGAGCGGCGCGGCGTCCACCCGGGCCCAGCCAGCCGGCGCGGCGGGGGCGGGTCCAGGA
TCTTCGCGGATCTTCGCGATCTTCCATTCTCAGGCGGGAGCCGGAGTCCGGGCGCCCGGGATGGGCTGGG
CCCGGCCATGGCAAGCGCGGCCTGCCCGGGCCCGGGGGACCCTGCCCTGGGTGAAGGCAGGGCCCCGGCC
TGGGGGCCCCGGCCCCGGGCCGGGAAACCCCCCCCACCTGGCGGGGGCCCGGGAAACGGGATTTTAAAAA
GTTAAGGAACCACGTCCCCCCAGATTTTGCTCTTAAGAATTCGAAAAACCGGCCAAAT
Kinase Domain Sequence
>SC323413 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMMSRSGWMYYWCGTGCGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTT
TAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCCGCGGC
AAGCCAGCCCGCCGGCGCCCCGGAGCGCTGCCGCCACCGTCACCGCCACCGCCCGGTCCAGGCCCAAGCC
CAGGCCCTGCGCGGAGCGGAGCGGCGCGGCGTCCACCCGGGCCCA
Restriction Sites Please inquire |
ACCN NM_006285
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006285.2, NP_006276.2
RefSeq Size 2559 bp
RefSeq ORF 1881 bp
Locus ID 7016
UniProt ID Q15569
Cytogenetics 9p13.3
Protein Families Druggable Genome, Protein Kinase
Summary This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain and a C-terminal proline-rich domain. Its protein kinase domain is most closely related to those of the LIM motif-containing protein kinases (LIMKs). The encoded protein can phosphorylate myelin basic protein and histone in vitro. The testicular germ cell-specific expression and developmental pattern of expression of the mouse gene suggests that this gene plays an important role at and after the meiotic phase of spermatogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Dec 2015
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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"NM_006285" in other vectors (8)
SKU Description Size Price
RC207560 TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$583.00
RC207560L1 Lenti-ORF clone of TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RC207560L2 Lenti-ORF clone of TESK1 (mGFP-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RC207560L3 Lenti-ORF clone of TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RC207560L4 Lenti-ORF clone of TESK1 (mGFP-tagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$883.00
RG207560 TESK1 (tGFP-tagged) - Human testis-specific kinase 1 (TESK1) 10 ug
$783.00
SC116192 TESK1 (untagged)-Human testis-specific kinase 1 (TESK1) 10 ug
$584.00
SC123458 TESK1 (untagged)-Human testis-specific kinase 1 (cDNA clone MGC:45208 IMAGE:5170888), complete cds 10 ug
$781.00

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