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Host cell factor C2 (HCFC2) (NM_013320) Human Tagged ORF Clone

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SKU
RC207543
HCFC2 (Myc-DDK-tagged)-Human host cell factor C2 (HCFC2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $725.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Host cell factor C2
Synonyms HCF-2; HCF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207543 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCCCAGCCTCCTCAACTGGAGGCGAGTTTCTTCCTTCACGGGGCCGGTCCCCCGCGCCCGGC
ACGGACACCGAGCGGTGGCCATCCGGGAGCTGATGATCATCTTTGGAGGGGGAAATGAGGGCATCGCGGA
TGAGCTGCACGTCTACAACACGGCTACGAATCAGTGGTTTCTGCCAGCTGTTAGAGGAGATATCCCTCCA
GGCTGTGCTGCCCATGGATTTGTCTGTGATGGTACCAGAATATTAGTATTTGGGGGAATGGTTGAATATG
GAAGATACAGCAATGAGTTATATGAGTTACAAGCAAGTCGTTGGTTATGGAAAAAAGTGAAACCCCATCC
CCCTCCTTCTGGTTTACCTCCTTGTCCTCGGCTTGGACATAGCTTCTCTTTATATGGTAACAAATGCTAT
TTGTTTGGTGGCCTGGCAAACGAAAGCGAAGATTCAAACAATAATGTTCCCAGATATTTAAATGATTTTT
ATGAGTTGGAGCTACAGCATGGCTCTGGTGTTGTGGGTTGGAGCATTCCAGTGACTAAAGGGGTTGTGCC
TTCTCCAAGAGAATCCCACACAGCTGTTATATATTGCAAAAAAGATTCTGGAAGTCCTAAAATGTATGTT
TTTGGTGGAATGTGTGGTGCTCGCCTGGATGACCTATGGCAGCTTGACTTAGAAACTATGTCATGGTCAA
AACCAGAAACTAAAGGGACAGTGCCACTTCCACGAAGCCTTCATACAGCCAGTGTTATAGGAAACAAGAT
GTACATTTTTGGTGGATGGGTCCCACATAAGGGGGAAAATACTGAGACTTCACCTCATGATTGTGAATGG
AGATGTACCAGTTCATTTTCTTACCTAAATCTGGATACAACAGAGTGGACCACCCTAGTATCAGATTCTC
AGGAAGATAAAAAAAATTCAAGACCAAGACCAAGAGCTGGCCACTGTGCTGTTGCAATCGGCACTCGATT
GTATTTTTGGAGTGGAAGAGATGGCTACAAAAAAGCACTGAATAGTCAAGTTTGCTGCAAGGATCTTTGG
TATCTTGATACTGAGAAACCACCGGCACCATCTCAAGTACAGCTGATCAAAGCCACTACCAACTCCTTTC
ATGTCAAGTGGGATGAAGTGTCTACAGTTGAGGGCTATCTTTTGCAGTTGAGTACAGACTTGCCATACCA
AGCTGCATCATCAGATTCTTCAGCAGCACCAAATATGCAAGGAGTCAGGATGGACCCTCACAGACAAGGC
AGTAATAACATCGTTCCTAACAGTATCAATGATACAATAAACAGCACAAAAACTGAACAGCCAGCCACAA
AAGAAACTTCAATGAAAAACAAACCAGACTTTAAAGCACTGACGGATTCTAATGCCATTTTATATCCATC
TTTGGCATCAAATGCTTCTAATCATAATAGTCATGTGGTGGATATGCTAAGGAAAAATGAAGGTCCTCAC
ACTTCAGCAAATGTAGGTGTTCTAAGTAGTTGCCTGGATGTAAGAACAGTAATTCCTGAAACATCTGTAT
CCAGTACTGTTTCCAGCACACAAACTATGGTAACCCAGCAGACCATTAAAACTGAATCATCCAGTACAAA
TGGGGCAGTTGTTAAAGATGAAACTTCACTAACAACATTCAGTACCAAATCTGAAGTTGATGAAACATAT
GCACTGCCTGCAACGAAGATCAGCCGTGTAGAGACACATGCTACAGCAACGCCGTTTTCTAAAGAGACTC
CTTCAAATCCAGTGGCCACAGTGAAAGCGGGAGAACGACAATGGTGTGATGTGGGAATTTTTAAAAATAA
TACAGCTTTGGTGAGCCAGTTTTATTTGCTGCCAAAAGGGAAGCAAAGCATCTCAAAGGTAGGAAATGCA
GATGTACCTGACTACAGCTTGCTTAAGAAACAAGATCTTGTTCCAGGCACAGGATACAGATTCAGGGTTG
CTGCAATCAATGGTTGTGGGATAGGTCCTTTCAGCAAAATCAGTGAATTTAAAACTTGTATTCCTGGTTT
TCCTGGAGCTCCTTCTGCAGTCAGAATTTCAAAGAATGTTGAAGGTATCCACCTTTCCTGGGAACCTCCA
ACCTCACCTTCTGGAAATATTTTGGAATATTCAGCCTACTTGGCTATCCGCACAGCACAGATACAAGATA
ATCCAAGTCAACTTGTGTTCATGAGGATTTATTGTGGTCTTAAGACATCATGTATAGTAACTGCTGGGCA
ACTTGCAAATGCACATATTGATTATACATCCAGGCCTGCCATTGTGTTCAGGATATCAGCAAAGAATGAA
AAGGGATATGGACCAGCTACACAAGTTCGGTGGCTTCAAGGTAACAATAAGAAAGCACCTTTAAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207543 protein sequence
Red=Cloning site Green=Tags(s)

MAAPSLLNWRRVSSFTGPVPRARHGHRAVAIRELMIIFGGGNEGIADELHVYNTATNQWFLPAVRGDIPP
GCAAHGFVCDGTRILVFGGMVEYGRYSNELYELQASRWLWKKVKPHPPPSGLPPCPRLGHSFSLYGNKCY
LFGGLANESEDSNNNVPRYLNDFYELELQHGSGVVGWSIPVTKGVVPSPRESHTAVIYCKKDSGSPKMYV
FGGMCGARLDDLWQLDLETMSWSKPETKGTVPLPRSLHTASVIGNKMYIFGGWVPHKGENTETSPHDCEW
RCTSSFSYLNLDTTEWTTLVSDSQEDKKNSRPRPRAGHCAVAIGTRLYFWSGRDGYKKALNSQVCCKDLW
YLDTEKPPAPSQVQLIKATTNSFHVKWDEVSTVEGYLLQLSTDLPYQAASSDSSAAPNMQGVRMDPHRQG
SNNIVPNSINDTINSTKTEQPATKETSMKNKPDFKALTDSNAILYPSLASNASNHNSHVVDMLRKNEGPH
TSANVGVLSSCLDVRTVIPETSVSSTVSSTQTMVTQQTIKTESSSTNGAVVKDETSLTTFSTKSEVDETY
ALPATKISRVETHATATPFSKETPSNPVATVKAGERQWCDVGIFKNNTALVSQFYLLPKGKQSISKVGNA
DVPDYSLLKKQDLVPGTGYRFRVAAINGCGIGPFSKISEFKTCIPGFPGAPSAVRISKNVEGIHLSWEPP
TSPSGNILEYSAYLAIRTAQIQDNPSQLVFMRIYCGLKTSCIVTAGQLANAHIDYTSRPAIVFRISAKNE
KGYGPATQVRWLQGNNKKAPLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013320
ORF Size 2376 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013320.3
RefSeq Size 5743 bp
RefSeq ORF 2379 bp
Locus ID 29915
UniProt ID Q9Y5Z7
Cytogenetics 12q23.3
Domains FN3, Kelch
Protein Families Transcription Factors
MW 86.8 kDa
Summary This gene encodes one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP16 through a beta-propeller domain. The original Herpes host cell factor, however, is effective at initiating viral infection while the encoded protein is not. Transcripts of varying length due to alternative polyadenylation signals have been described. [provided by RefSeq, Jul 2008]
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Documents
Resources
"NM_013320" in other vectors (4)
SKU Description Size Price
RC207543L3 Lenti ORF clone of Human host cell factor C2 (HCFC2), Myc-DDK-tagged 10 ug
$1,025.00
RC207543L4 Lenti ORF clone of Human host cell factor C2 (HCFC2), mGFP tagged 10 ug
$1,025.00
RG207543 HCFC2 (tGFP-tagged) - Human host cell factor C2 (HCFC2) 10 ug
$789.00 $925.00
SC108617 HCFC2 (untagged)-Human host cell factor C2 (HCFC2) 10 ug
$726.00

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