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ADAR1 (ADAR) (NM_001111) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC207522
ADAR (Myc-DDK-tagged)-Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$589.00 $1,600.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ADAR1 or NM_001111"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ADAR1
Synonyms ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001111
ORF Size 3678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001111.5
RefSeq Size 6692 bp
RefSeq ORF 3681 bp
Locus ID 103
UniProt ID P55265
Cytogenetics 1q21.3
Domains A_deamin, DSRM, z-alpha
Protein Families Druggable Genome
Protein Pathways Cytosolic DNA-sensing pathway
MW 136 kDa
Summary This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2010
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"NM_001111" in other vectors (6)
SKU Description Size Price
RC207522L1 Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, Myc-DDK-tagged 10 ug
$1,900.00
RC207522L2 Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, mGFP tagged 10 ug
$1,900.00
RC207522L3 Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, Myc-DDK-tagged 10 ug
$1,900.00
RC207522L4 Lenti ORF clone of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1, mGFP tagged 10 ug
$1,900.00
RG207522 ADAR (tGFP-tagged) - Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1 10 ug
$789.00 $1,800.00
SC119438 ADAR (untagged)-Human adenosine deaminase, RNA-specific (ADAR), transcript variant 1 10 ug
$1,602.00

Plasmid Map for RC207522

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207522
Circular map for RC207522

file-alt Citations

RNA modifications can affect RNase H1-mediated PS-ASO activity
Doxtader Lacy, Liang, Zhang et al
Mol Ther Nucleic Acids (2022) 28, 814-828 DOI: 10.1016/j.omtn.2022.05.024
Cited by 33
AI Summary: In this study, OriGene’s RNASEH1 cDNA clone (Cat# SC119513) was used to express recombinant RNase H1, enabling the authors to assess how RNA modifications influence RNase H1-mediated cleavage activity of PS-ASOs.
FigureFigureFigure
RNA modifications can affect RNase H1-mediated PS-ASO activity
Doxtader Lacy, Liang, Zhang et al
Molecular Therapy - Nucleic Acids (2022) 28, 814-828 DOI: 10.1016/j.omtn.2022.05.024
Cited by 33
AI Summary: OriGene's ADAR1 overexpression plasmid (OriGene #RC207522) was used to generate a stable HEK 293 cell line, enabling the study of how ADAR1-mediated RNA modifications influence PS-ASO binding and RNase H1 cleavage activity in this research.
Snippet: C, with 5% CO2, in DMEM medium supplemented with 10% fetal bovine serum, 0.1 μg/mL streptomycin, and 100 units/mL penicillin. To generate the stable cell line overexpressing ADAR1, 1 μg plasmid (Origene #RC207522) was transfected into HEK 293 cells and a stable cell line was selected by passaging cells in the presence of 0.5 mg/mL Geneticin (Thermo Fisher). siRNA treatment Two siRNAs target
The Role of ADAR1 and ADAR2 in the Regulation of miRNA-21 in Idiopathic Pulmonary Fibrosis
Díaz-Piña, Ordoñez-Razo, Montes et al
Lung (2018)
AI Summary: OriGene products—specifically the ADAR1 (RC207522) and ADAR2 (RC209073) overexpression vectors and the empty control vector (PS100001)—were used to transfect IPF fibroblasts, enabling the study of how ADAR1 and ADAR2 regulate miRNA-21 expression in idiopathic pulmonary fibrosis.
Snippet: ADAR1 and ADAR2 Transfection We transfected the specific vectors for the overexpression of ADAR1 (RC207522) or ADAR2 (RC209073, OriGene, Maryland), in two primary cultures of IPF fibroblasts As a negative control, the empty vec- tor was used (PS100001, OriGene
PTBP1 induces ADAR1 p110 isoform expression through IRES-like dependent translation control and influences cell proliferation in gliomas
Yang, Hu, Lin et al
Cell. Mol. Life Sci. (2015)
Cited by 46
AI Summary: OriGene contributed human ADAR (NM_001111) cDNA clone plasmids, which were used to investigate ADAR1 expression and translational regulation in glioma cells.
Snippet: Plasmid constructs and luciferase assays. Human ADAR (NM_001111) cDNA clone plasmids were obtained from Origene. Fragments (1–885 nt) of ADAR1 and the 5′UTR of UNR were PCR amplified and cloned into the pGL3-basic and pHRF vectors. ... Cite SaveSaving
Showing 1-4 of 4 papers.
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