EEF2K (NM_013302) Human Tagged Lenti ORF Clone
- LentiORF®
SKU
RC207496L2
Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), mGFP tagged
$964.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | EEF2K |
| Synonyms | CaMKIII; eEF-2K; HSU93850 |
| Vector | pLenti-C-mGFP |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
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| ACCN | NM_013302 |
| ORF Size | 2175 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013302.3 |
| RefSeq Size | 7412 bp |
| RefSeq ORF | 2178 bp |
| Locus ID | 29904 |
| UniProt ID | O00418 |
| Cytogenetics | 16p12.2 |
| Domains | Alpha_kinase |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 82.2 kDa |
| Summary | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013302" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207496 | EEF2K (Myc-DDK-tagged)-Human eukaryotic elongation factor-2 kinase (EEF2K) | 10 ug |
$664.00
|
|
| RC207496L1 | Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), Myc-DDK-tagged | 10 ug |
$964.00
|
|
| RC207496L3 | Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), Myc-DDK-tagged | 10 ug |
$964.00
|
|
| RC207496L4 | Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), mGFP tagged | 10 ug |
$964.00
|
|
| RG207496 | EEF2K (tGFP-tagged) - Human eukaryotic elongation factor-2 kinase (EEF2K) | 10 ug |
$789.00
$864.00
|
|
| SC126564 | EEF2K (untagged)-Human eukaryotic elongation factor-2 kinase (EEF2K) | 10 ug |
$665.00
|
|
| SC323690 | EEF2K (untagged)-Kinase deficient mutant (K170M) of Human eukaryotic elongation factor-2 kinase (EEF2K) | 10 ug |
$665.00
|
|
Plasmid Map for RC207496L2
Download sequence for
Cloning scheme for RC207496L2
Circular map for RC207496L2
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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