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EEF2K (NM_013302) Human Untagged Clone

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SKU
SC126564
EEF2K (untagged)-Human eukaryotic elongation factor-2 kinase (EEF2K)
  $665.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EEF2K
Synonyms CaMKIII; eEF-2K; HSU93850
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC126564 sequence for NM_013302 edited (data generated by NextGen Sequencing)
ATGGCAGACGAAGATCTCATCTTCCGCCTGGAAGGCGTTGATGGCGGCCAGTCCCCCCGA
GCTGGCCGTGATGGTGATTCTGATGGGGACAGCGACGATGAGGAAGGTTACTTCATCTGC
CCCATCACGGATGACCCAAGCTCGAACCAGAATGTCAATTCCAAGGTTAATAAGTACTAC
AGCAACCTAACAAAAAGTGAGCGGTATAGCTCCAGCGGGTCCGCGGCAAACTCCTTCCAC
TTCAAGGAAGCCTGGAAGCACGCAATCCAGAAGGCCAAGCACATGCCCGACCCCTGGGCT
GAGTTCCACCTGGAAGATATTGCCACCGAACGTGCTACTCGACACAGGTACAACGCCGTC
ACCGGGGAATGGCTGGATGATGAAGTTCTGATCAAGATGGCATCTCAGCCCTTCGGCCGA
GGAGCAATGAGGGAGTGCTTCCGGACGAAGAAGCTCTCCAACTTCTTGCATGCCCAGCAG
TGGAAGGGAGCCTCCAACTACGTGGCGAAGCGCTACATCGAGCCCGTAGACCGGGATGTG
TACTTTGAGGACGTGCGTCTACAGATGGAGGCCAAGCTCTGGGGGGAGGAGTATAATCGG
CACAAGCCCCCCAAGCAGGTGGACATCATGCAGATGTGCATCATCGAGCTGAAGGACAGA
CCGGGCAAGCCCCTCTTCCACCTGGAGCACTACATCGAGGGCAAGTACATCAAGTACAAC
TCCAACTCTGGCTTTGTCCGCGATGACAACATCCGCCTGACGCCGCAGGCCTTCAGCCAC
TTCACTTTTGAGCGTTCCGGCCATCAGCTGATAGTGGTGGACATCCAGGGAGTTGGGGAT
CTCTACACTGACCCACAGATCCACACGGAGACGGGCACTGACTTTGGAGACGGCAACCTA
GGTGTCCGCGGGATGGCGCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGCGAGAGC
ATGGGCCTTGCTCCCTTTGACCTCTCGCCCCGGGAGAGGGATGCAGTGAATCAGAACACC
AAGCTGCTGCAATCAGCCAAGACCATCTTGAGAGGAACAGAGGAAAAATGTGGGAGCCCC
CGAGTAAGGACCCTCTCTGGGAGCCGGCCACCCCTGCTCCGTCCCCTTTCAGAGAACTCT
GGAGACGAGAACATGAGCGACGTGACCTTCGACTCTCTCCCTTCTTCCCCATCTTCGGCC
ACACCACACAGCCAGAAGCTAGACCACCTCCATTGGCCAGTGTTCAGTGACCTCGATAAC
ATGGCATCCAGAGACCATGATCATCTAGACAACCACCGGGAGTCTGAGAATAGTGGGGAC
AGCGGATACCCCAGTGAGAAGCGGGGTGAGCTGGATGACCCTGAGCCCCGAGAACATGGC
CACTCATACAGTAATCGGAAGTACGAGTCTGACGAAGACAGCCTGGGCAGCTCTGGACGG
GTATGTGTAGAGAAGTGGAATCTCCTCAACTCCTCCCGCCTCCACCTGCCGAGGGCTTCG
GCCGTGGCCCTGGAAGTGCAAAGGCTTAATGCTCTGGACCTCGAAAAGAAAATCGGGAAG
TCCATTTTGGGGAAGGTCCATCTGGCCATGGTGCGCTACCACGAGGGTGGGCGCTTCTGC
GAGAAGGGCGAGGAGTGGGACCAGGAGTCGGCTGTCTTCCACCTGGAGCACGCAGCCAAC
CTGGGCGAGCTGGAGGCCATCGTGGGCCTGGGACTCATGTACTCGCAGTTGCCTCATCAC
ATCCTAGCCGATGTCTCTCTGAAGGAGACAGAAGAGAACAAAACCAAAGGATTTGATTAC
TTACTAAAGGCCGCTGAAGCTGGCGACAGGCAGTCCATGATCCTAGTGGCGCGAGCTTTT
GACTCTGGCCAGAACCTCAGCCCGGACAGGTGCCAAGACTGGCTAGAGGCCCTGCACTGG
TACAACACTGCCCTGGAGATGACGGACTGTGATGAGGGCGGTGAGTACGACGGAATGCAG
GACGAGCCCCGGTACATGATGCTGGCCAGGGAGGCCGAGATGCTGTTCACAGGAGGCTAC
GGGCTGGAGAAGGACCCGCAGAGATCAGGGGACTTGTATACCCAGGCAGCAGAGGCAGCG
ATGGAAGCCATGAAGGGCCGACTGGCCAACCAGTACTACCAAAAGGCTGAAGAGGCCTGG
GCCCAGATGGAGGAGTAA

Clone variation with respect to NM_013302.3
68 a=>g;223 c=>g;489 c=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_013302 unedited
GGACGCGAACTCGGCACCAGTAGAGCCATCACTATTGTCTGGAGTGGTGCAGGCCTTGCG
AGGCCGCCTGTTGCCCAGCCTGGGGCCCCCAGGACCAACCCGGAGCCCCCCTGGACCCCA
CACAGCAGTCCTGGCCCTGGAAGATGAGGACGATGTGCTACTGGTGCCACTGGCTGAGCC
GGGGGTGTGGGTAGCTGAGGCAGAGGATGAGCCACTGCTTACCTGAGGGGACCTGGGGGC
TCTACTGAGGCCTCTCCCCTGGGGGCTCTACTCATAGTGGCACAACCTTTTAGAGGTGGG
TCAGCCCTCCGGGCCGCGAGCCTCTGCCAACTCCTCTGGACCCTCGCGGCCGTGGGCAGC
GGCTGCCGCGCCTGTCTGCCCGAGGGAGGACCTTCGCCTCTGCATTTGTCCAGTAACTCT
GGCTGTGCCGGATACTGCTTGGGTAAAACGGGCACCCCAGGAACATGGCAGACGAAGATC
TCATCTTCCGCCTGGAAGGCGTTGATGGCGGCCAGTCCCCCCGAGCTGGCCGTGATGGTG
ATTCTGATGGGGACAGCGACGATGAGGAAGGTTACTTCATCTGCCCCATCACGGATGACC
CAAGCTCGAACCAGAATGTCAATTCCAAGGTTAATAAGTACTACAGCAACCTAACAAAAA
GTGAGCGGTATAGCTCCAGCGGGTCCGCGGCAAACTTCTTTCACTTCAAGGAAGCC
Restriction Sites Please inquire |
ACCN NM_013302
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013302.2, NP_037434.1
RefSeq Size 3599 bp
RefSeq ORF 2178 bp
Locus ID 29904
UniProt ID O00418
Cytogenetics 16p12.2
Domains Alpha_kinase
Protein Families Druggable Genome, Protein Kinase
Summary This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. provided by RefSeq, Jul 2008
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"NM_013302" in other vectors (7)
SKU Description Size Price
RC207496 EEF2K (Myc-DDK-tagged)-Human eukaryotic elongation factor-2 kinase (EEF2K) 10 ug
$664.00
RC207496L1 Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), Myc-DDK-tagged 10 ug
$964.00
RC207496L2 Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), mGFP tagged 10 ug
$964.00
RC207496L3 Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), Myc-DDK-tagged 10 ug
$964.00
RC207496L4 Lenti ORF clone of Human eukaryotic elongation factor-2 kinase (EEF2K), mGFP tagged 10 ug
$964.00
RG207496 EEF2K (tGFP-tagged) - Human eukaryotic elongation factor-2 kinase (EEF2K) 10 ug
$789.00 $864.00
SC323690 EEF2K (untagged)-Kinase deficient mutant (K170M) of Human eukaryotic elongation factor-2 kinase (EEF2K) 10 ug
$665.00

file-alt Citations

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