OriGene Logo

WDR75 (NM_032168) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC207339
WDR75 (Myc-DDK-tagged)-Human WD repeat domain 75 (WDR75)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $760.00
In Stock*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA359021 WDR75 Antibody - N-terminal region 100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags 30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA) 5 x 200 ul
$160.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each 100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug 10 ug
$650.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL 5 ml
$720.00

Other products for "WDR75 or NM_032168"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDR75
Synonyms NET16; UTP17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207339 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGAGGAGGAGAACATCCGCGTGGTTCGTTGTGGCGGCAGCGAGTTGAACTTTAGGAGAGCTGTGT
TCTCTGCAGATTCTAAGTATATCTTCTGTGTCTCTGGAGACTTTGTTAAAGTTTACAGCACAGTTACAGA
AGAGTGTGTACACATACTGCATGGACACAGAAATCTGGTGACTGGAATCCAGCTTAACCCCAACAACCAT
CTACAGCTGTATTCTTGTTCCCTTGATGGCACAATTAAACTGTGGGACTATATAGATGGCATCTTAATAA
AGACTTTCATAGTTGGATGTAAACTTCATGCCCTCTTTACTCTTGCCCAAGCTGAGGATTCTGTCTTTGT
TATAGTGAATAAAGAAAAACCAGATATATTTCAGCTGGTTTCAGTGAAACTGCCAAAATCCTCAAGCCAG
GAAGTAGAAGCCAAGGAGCTGTCCTTTGTTTTGGATTACATAAACCAGTCACCCAAGTGCATTGCCTTTG
GAAACGAGGGAGTATATGTTGCTGCAGTACGGGAATTTTACTTGTCTGTTTATTTTTTCAAAAAGAAAAC
AACATCAAGGTTTACTTTATCATCATCAAGAAATAAGAAGCATGCTAAAAACAATTTTACGTGTGTAGCA
TGTCACCCAACGGAAGACTGCATCGCATCTGGTCACATGGATGGCAAAATTCGTCTTTGGAGGAATTTTT
ATGATGATAAGAAATATACGTACACATGTTTACATTGGCACCATGATATGGTTATGGATTTGGCTTTTTC
AGTGACAGGCACCAGTCTGCTGAGTGGCGGTCGTGAATCTGTACTTGTAGAGTGGCGCGATGCAACAGAG
AAGAATAAGGAGTTTCTCCCGCGTTTAGGAGCTACTATTGAACATATCTCAGTCTCGCCTGCAGGAGATT
TATTCTGCACTTCTCACTCTGATAATAAGATAATAATTATTCACCGAAACCTTGAAGCATCCGCAGTAAT
TCAAGGCCTAGTGAAAGATAGGAGTATCTTCACTGGTTTGATGATTGATCCAAGAACTAAAGCTTTGGTT
TTGAATGGAAAACCTGGCCACCTGCAGTTTTATTCTCTCCAGAGTGATAAACAGTTATACAATTTAGATA
TTATACAGCAAGAATATATTAATGATTATGGTCTGATCCAAATTGAACTAACAAAGGCTGCATTTGGCTG
CTTTGGTAACTGGCTTGCAACAGTGGAACAGCGGCAAGAAAAGGAAACTGAGCTTGAATTGCAAATGAAA
CTGTGGATGTATAATAAGAAAACACAAGGGTTTATTCTTAACACTAAAATTAACATGCCACACGAAGACT
GCATTACAGCTCTCTGTTTCTGTAATGCAGAAAAATCTGAACAGCCCACCTTGGTTACAGCTAGCAAAGA
TGGTTACTTCAAAGTATGGATATTAACAGATGACTCTGACATATACAAAAAAGCTGTTGGCTGGACCTGT
GACTTTGTTGGTAGTTATCACAAGTATCAAGCAACTAACTGTTGTTTCTCCGAAGATGGTTCTTTACTAG
CAGTTAGTTTTGAGGAAATAGTCACAATATGGGATTCTGTAACATGGGAACTTAAATGTACATTTTGCCA
ACGAGCTGGGAAAATAAGGCACCTTTGCTTTGGGAGATTGACGTGTTCAAAGTATCTACTTGGTGCTACT
GAAAATGGCATTCTTTGCTGTTGGAATCTGCTGAGCTGTGCATTGGAGTGGAATGCAAAATTAAATGTTA
GAGTTATGGAACCCGATCCTAATTCAGAGAATATTGCTGCAATCTCTCAGTCTTCAGTGGGTTCAGACTT
GTTTGTATTTAAACCTAGTGAGCCAAGGCCATTGTATATTCAAAAGGGTATCTCCAGAGAGAAAGTCCAG
TGGGGAGTGTTTGTTCCACGAGATGTCCCTGAATCCTTCACCTCAGAAGCTTACCAGTGGCTAAATAGAT
CCCAGTTTTACTTCCTAACAAAATCACAGAGTTTATTGACATTCAGTACAAAGTCTCCAGAAGAAAAACT
CACACCAACAAGCAAACAGCTGCTAGCAGAAGAAAGTCTTCCCACAACCCCATTTTATTTCATATTGGGA
AAACACAGGCAACAGCAGGATGAAAAACTAAACGAAACTTTAGAGAATGAGCTGGTACAACTACCCTTAA
CAGAAAACATACCCGCAATTAGTGAGCTTCTTCACACTCCAGCCCATGTCCTGCCATCTGCTGCTTTCCT
GTGCTCCATGTTTGTAAATTCATTGCTGCTGTCTAAAGAGACTAAGAGTGCTAAGGAAATTCCTGAAGAT
GTAGATATGGAAGAAGAAAAAGAAAGTGAAGATTCAGATGAAGAAAATGATTTTACCGAAAAAGTCCAGG
ATACAAGTAACACAGGTTTAGGAGAAGACATTATACATCAGTTGTCAAAATCTGAAGAAAAAGAACTGAG
AAAATTTAGGAAAATAGACTACAGCTGGATAGCTGCCCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207339 protein sequence
Red=Cloning site Green=Tags(s)

MVEEENIRVVRCGGSELNFRRAVFSADSKYIFCVSGDFVKVYSTVTEECVHILHGHRNLVTGIQLNPNNH
LQLYSCSLDGTIKLWDYIDGILIKTFIVGCKLHALFTLAQAEDSVFVIVNKEKPDIFQLVSVKLPKSSSQ
EVEAKELSFVLDYINQSPKCIAFGNEGVYVAAVREFYLSVYFFKKKTTSRFTLSSSRNKKHAKNNFTCVA
CHPTEDCIASGHMDGKIRLWRNFYDDKKYTYTCLHWHHDMVMDLAFSVTGTSLLSGGRESVLVEWRDATE
KNKEFLPRLGATIEHISVSPAGDLFCTSHSDNKIIIIHRNLEASAVIQGLVKDRSIFTGLMIDPRTKALV
LNGKPGHLQFYSLQSDKQLYNLDIIQQEYINDYGLIQIELTKAAFGCFGNWLATVEQRQEKETELELQMK
LWMYNKKTQGFILNTKINMPHEDCITALCFCNAEKSEQPTLVTASKDGYFKVWILTDDSDIYKKAVGWTC
DFVGSYHKYQATNCCFSEDGSLLAVSFEEIVTIWDSVTWELKCTFCQRAGKIRHLCFGRLTCSKYLLGAT
ENGILCCWNLLSCALEWNAKLNVRVMEPDPNSENIAAISQSSVGSDLFVFKPSEPRPLYIQKGISREKVQ
WGVFVPRDVPESFTSEAYQWLNRSQFYFLTKSQSLLTFSTKSPEEKLTPTSKQLLAEESLPTTPFYFILG
KHRQQQDEKLNETLENELVQLPLTENIPAISELLHTPAHVLPSAAFLCSMFVNSLLLSKETKSAKEIPED
VDMEEEKESEDSDEENDFTEKVQDTSNTGLGEDIIHQLSKSEEKELRKFRKIDYSWIAAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032168
ORF Size 2490 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032168.1, NP_115544.1
RefSeq Size 2777 bp
RefSeq ORF 2493 bp
Locus ID 84128
UniProt ID Q8IWA0
Cytogenetics 2q32.2
MW 94.5 kDa
Summary Ribosome biogenesis factor. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_032168" in other vectors (6)
SKU Description Size Price
RC207339L1 Lenti ORF clone of Human WD repeat domain 75 (WDR75), Myc-DDK-tagged 10 ug
$1,060.00
RC207339L2 Lenti ORF clone of Human WD repeat domain 75 (WDR75), mGFP tagged 10 ug
$1,060.00
RC207339L3 Lenti ORF clone of Human WD repeat domain 75 (WDR75), Myc-DDK-tagged 10 ug
$1,060.00
RC207339L4 Lenti ORF clone of Human WD repeat domain 75 (WDR75), mGFP tagged 10 ug
$1,060.00
RG207339 WDR75 (tGFP-tagged) - Human WD repeat domain 75 (WDR75) 10 ug
$789.00 $960.00
SC127732 WDR75 (untagged)-Human WD repeat domain 75 (WDR75) 10 ug
$761.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.