CRY2 (NM_021117) Human Tagged ORF Clone

SKU RC207337

CRY2 (Myc-DDK-tagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CRY2
• Synonyms: HCRY2; PHLL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207337 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCGACTGTGGCGACGGCGGCAGCTGTGGCCCCGGCGCCAGCGCCCGGCACGGACAGCGCCTCTT; CGGTGCACTGGTTCCGCAAAGGGCTGCGACTCCACGACAACCCGGCGTTGCTGGCGGCCGTGCGCGGGGC; GCGCTGCGTGCGCTGCGTTTACATTCTCGACCCGTGGTTCGCGGCCTCCTCCTCAGTCGGGATCAACCGA; TGGAGGTTCCTACTTCAGTCTCTGGAAGATTTGGACACAAGTTTAAGGAAACTGAACTCCCGCCTGTTTG; TAGTCCGGGGACAGCCAGCCGACGTGTTCCCAAGGCTGTTCAAGGAATGGGGAGTGACCCGCTTGACCTT; TGAATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATCATGAAGATGGCCAAGGAGGCTGGT; GTGGAAGTAGTGACGGAGAATTCTCATACCCTCTATGACCTGGACAGGATCATTGAGCTGAATGGGCAGA; AGCCACCCCTTACATACAAGCGCTTTCAGGCCATCATCAGCCGCATGGAGCTGCCCAAGAAGCCAGTGGG; CTTGGTGACCAGCCAGCAGATGGAGAGCTGCAGGGCCGAGATCCAGGAGAACCACGACGAGACCTACGGC; GTGCCCTCCCTGGAGGAGCTGGGGTTCCCCACTGAAGGACTTGGTCCAGCTGTCTGGCAGGGAGGAGAGA; CAGAAGCTCTGGCCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCCCG; AATGAACGCCAACTCCCTCCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGTTGTCTCTCC; TGCCGCCTCTTCTACTACCGCCTGTGGGACCTGTATAAAAAGGTGAAGCGGAACAGCACACCTCCCCTCT; CCCTATTTGGGCAACTCCTATGGCGAGAGTTCTTCTACACGGCAGCTACCAACAACCCCAGGTTTGACCG; CATGGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAATCCTGAGGCCCTGGCCAAGTGGGCT; GAGGGCAAGACAGGCTTCCCTTGGATTGATGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACC; ACCTGGCCCGGCATGCCGTGGCCTGCTTCCTGACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGT; CCGGGTATTTGATGAGCTGCTCCTGGATGCAGATTTCAGCGTGAACGCAGGCAGCTGGATGTGGCTGTCC; TGCAGTGCTTTCTTCCAGCAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGTCGCACGGACCCCA; GTGGGGACTACATCAGGCGATACCTGCCCAAATTGAAAGCGTTCCCCTCTCGATACATCTATGAGCCCTG; GAATGCCCCAGAGTCAATTCAGAAGGCAGCCAAGTGCATCATTGGTGTGGACTACCCACGGCCCATCGTC; AACCATGCCGAGACCAGCCGGCTTAACATTGAACGAATGAAGCAGATTTACCAGCAGCTTTCGCGCTACC; GGGGACTCTGTCTACTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCCAG; CTCGAGCCAGGCTGGCAGCATGAGCAGTGCAGGCCCAAGACCACTACCCAGTGGCCCAGCATCCCCCAAA; CGCAAGCTGGAAGCAGCCGAGGAACCACCTGGTGAAGAACTCAGCAAACGGGCCCGGGTGGCAGAGTTGC; CAACCCCAGAGCTGCCGAGCAAGGATGCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207337 protein sequence; Red=Cloning site Green=Tags(s); ; MAATVATAAAVAPAPAPGTDSASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINR; WRFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAG; VEVVTENSHTLYDLDRIIELNGQKPPLTYKRFQAIISRMELPKKPVGLVTSQQMESCRAEIQENHDETYG; VPSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLS; CRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWA; EGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLS; CSAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKAFPSRYIYEPWNAPESIQKAAKCIIGVDYPRPIV; NHAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPSSSQAGSMSSAGPRPLPSGPASPK; RKLEAAEEPPGEELSKRARVAELPTPELPSKDA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021117
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021117.5
• RefSeq Size: 4204 bp
• RefSeq ORF: 1782 bp
• Locus ID: 1408
• UniProt ID: Q49AN0
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• MW: 66.9 kDa
• Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]