UPP2 (NM_173355) Human Tagged ORF Clone

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SKU
RC207260
UPP2 (Myc-DDK-tagged)-Human uridine phosphorylase 2 (UPP2), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UPP2
Synonyms UDRPASE2; UP2; UPASE2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207260 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCAGTTATACCTGCCTCCAATAGGTCCATGAGATCTGACAGGAATACATATGTTGGAAAAAGGT
TTGTTCACGTTAAAAATCCTTACTTGGATTTGATGGATGAAGACATTCTCTATCACTTGGATTTGGGAAC
AAAAACACACAACCTACCAGCAATGTTTGGAGATGTAAAGTTTGTCTGTGTCGGTGGGAGCCCCAACAGA
ATGAAAGCATTTGCACTGTTTATGCACAAGGAGCTCGGGTTTGAGGAAGCTGAAGAAGACATAAAAGACA
TCTGTGCTGGGACAGACAGATACTGTATGTACAAAACCGGGCCTGTGCTCGCCATCAGTCACGGCATGGG
CATCCCCTCCATTTCTATTATGCTTCATGAACTCATCAAATTACTCCACCATGCACGGTGCTGCGATGTC
ACCATTATTAGAATCGGTACATCAGGGGGAATAGGGATTGCACCAGGGACTGTTGTAATAACGGATATAG
CTGTAGACTCCTTCTTTAAGCCCCGGTTTGAACAGGTCATTTTGGACAACATTGTCACCCGAAGTACTGA
ACTGGACAAAGAACTGTCTGAAGAACTGTTCAACTGTAGCAAAGAAATCCCCAACTTCCCAACCCTCGTT
GGACATACAATGTGTACCTATGATTTTTATGAAGGCCAAGGCCGACTAGATGGAGCACTGTGCTCCTTTT
CCAGAGAAAAAAAGTTAGACTACTTGAAGAGAGCATTTAAAGCTGGTGTCAGGAATATTGAAATGGAATC
TACAGTGTTTGCAGCTATGTGTGGACTCTGTGGTCTAAAAGCTGCTGTGGTCTGTGTGACACTTCTCGAC
AGACTCGACTGTGATCAGATCAACTTGCCTCATGATGTCCTGGTGGAGTACCAGCAACGGCCTCAGCTCC
TAATCTCCAACTTCATCAGACGGCGGCTTGGACTTTGTGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207260 protein sequence
Red=Cloning site Green=Tags(s)

MASVIPASNRSMRSDRNTYVGKRFVHVKNPYLDLMDEDILYHLDLGTKTHNLPAMFGDVKFVCVGGSPNR
MKAFALFMHKELGFEEAEEDIKDICAGTDRYCMYKTGPVLAISHGMGIPSISIMLHELIKLLHHARCCDV
TIIRIGTSGGIGIAPGTVVITDIAVDSFFKPRFEQVILDNIVTRSTELDKELSEELFNCSKEIPNFPTLV
GHTMCTYDFYEGQGRLDGALCSFSREKKLDYLKRAFKAGVRNIEMESTVFAAMCGLCGLKAAVVCVTLLD
RLDCDQINLPHDVLVEYQQRPQLLISNFIRRRLGLCD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173355
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173355.4
RefSeq Size 2413 bp
RefSeq ORF 954 bp
Locus ID 151531
UniProt ID O95045
Cytogenetics 2q24.1
Protein Pathways Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
MW 35.5 kDa
Summary Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows substrate specificity and accept uridine, deoxyuridine, and thymidine as well as the two pyrimidine nucleoside analogs 5-fluorouridine and 5-fluoro-2(')-deoxyuridine as substrates.[UniProtKB/Swiss-Prot Function]
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Documents
Resources
"NM_173355" in other vectors (4)
SKU Description Size Price
RC207260L3 Lenti ORF clone of Human uridine phosphorylase 2 (UPP2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC207260L4 Lenti ORF clone of Human uridine phosphorylase 2 (UPP2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG207260 UPP2 (tGFP-tagged) - Human uridine phosphorylase 2 (UPP2), transcript variant 1 10 ug
$489.00 $500.00
SC123316 UPP2 (untagged)-Human uridine phosphorylase 2 (UPP2), transcript variant 1 10 ug
$300.00

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