GPR6 (NM_005284) Human Tagged ORF Clone

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SKU
RC207204
GPR6 (Myc-DDK-tagged)-Human G protein-coupled receptor 6 (GPR6)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPR6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207204 representing NM_005284
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGCGAGCGCCGCCTCGCTCAACGACTCCCAGGTGGTGGTAGTGGCGGCCGAAGGAGCGGCGGCGG
CGGCCACAGCAGCAGGGGGGCCGGACACGGGCGAATGGGGACCCCCTGCTGCGGCGGCTCTAGGAGCCGG
CGGCGGAGCTAATGGGTCTCTGGAGCTGTCCTCGCAGCTGTCGGCTGGGCCACCGGGACTCCTGCTGCCA
GCGGTGAATCCGTGGGACGTGCTCCTGTGCGTGTCGGGGACAGTGATCGCTGGAGAAAACGCGCTGGTGG
TGGCGCTCATCGCGTCCACTCCGGCGCTGCGCACGCCCATGTTCGTGCTGGTAGGCAGCCTGGCCACCGC
TGACCTGTTGGCGGGCTGTGGCCTCATCTTGCACTTTGTGTTCCAGTACTTGGTGCCCTCGGAGACTGTG
AGTCTGCTCACGGTGGGCTTCCTCGTGGCCTCCTTCGCCGCCTCTGTCAGCAGCCTGCTGGCCATTACGG
TGGACCGCTACCTGTCCCTGTATAACGCGCTCACCTATTACTCGCGCCGGACCCTGTTGGGCGTGCACCT
CCTGCTTGCCGCCACTTGGACCGTGTCCCTAGGCCTGGGGCTGCTGCCCGTGCTGGGCTGGAACTGCCTG
GCAGAGCGCGCCGCCTGCAGCGTGGTGCGCCCGCTGGCGCGCAGCCACGTGGCTCTGCTCTCCGCCGCCT
TCTTCATGGTCTTCGGCATCATGCTGCACCTGTACGTGCGCATCTGCCAGGTGGTCTGGCGCCACGCGCA
CCAGATCGCGCTGCAGCAGCACTGCCTGGCGCCACCCCATCTCGCTGCCACCAGAAAGGGTGTGGGTACA
CTGGCTGTGGTGCTGGGCACTTTCGGCGCCAGCTGGCTGCCCTTCGCCATCTATTGCGTGGTGGGCAGCC
ATGAGGACCCGGCGGTCTACACTTACGCCACCCTGCTGCCCGCCACCTACAACTCCATGATCAATCCCAT
CATCTATGCCTTCCGCAACCAGGAGATCCAGCGCGCCCTGTGGCTCCTGCTCTGTGGCTGTTTCCAGTCC
AAAGTGCCCTTTCGTTCCAGGTCTCCCAGCGAGGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207204 representing NM_005284
Red=Cloning site Green=Tags(s)

MNASAASLNDSQVVVVAAEGAAAAATAAGGPDTGEWGPPAAAALGAGGGANGSLELSSQLSAGPPGLLLP
AVNPWDVLLCVSGTVIAGENALVVALIASTPALRTPMFVLVGSLATADLLAGCGLILHFVFQYLVPSETV
SLLTVGFLVASFAASVSSLLAITVDRYLSLYNALTYYSRRTLLGVHLLLAATWTVSLGLGLLPVLGWNCL
AERAACSVVRPLARSHVALLSAAFFMVFGIMLHLYVRICQVVWRHAHQIALQQHCLAPPHLAATRKGVGT
LAVVLGTFGASWLPFAIYCVVGSHEDPAVYTYATLLPATYNSMINPIIYAFRNQEIQRALWLLLCGCFQS
KVPFRSRSPSEV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005284
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005284.5
RefSeq Size 1667 bp
RefSeq ORF 1089 bp
Locus ID 2830
UniProt ID P46095, F1DAM6
Cytogenetics 6q21
Protein Families Druggable Genome, GPCR, Transmembrane
MW 37.7 kDa
Summary Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_005284" in other vectors (6)
SKU Description Size Price
RC207204L1 Lenti ORF clone of Human G protein-coupled receptor 6 (GPR6), Myc-DDK-tagged 10 ug
$757.00
RC207204L2 Lenti ORF clone of Human G protein-coupled receptor 6 (GPR6), mGFP tagged 10 ug
$757.00
RC207204L3 Lenti ORF clone of Human G protein-coupled receptor 6 (GPR6), Myc-DDK-tagged 10 ug
$757.00
RC207204L4 Lenti ORF clone of Human G protein-coupled receptor 6 (GPR6), mGFP tagged 10 ug
$757.00
RG207204 GPR6 (tGFP-tagged) - Human G protein-coupled receptor 6 (GPR6) 10 ug
$489.00 $657.00
SC116840 GPR6 (untagged)-Human G protein-coupled receptor 6 (GPR6) 10 ug
$457.00

Plasmid Map for RC207204

Download sequence for

Cloning scheme for RC207204

Circular map for RC207204

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