YTHDF1 (NM_017798) Human Tagged ORF Clone
$520.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | YTHDF1 |
| Synonyms | C20orf21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_017798 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017798.4 |
| RefSeq Size | 3277 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 54915 |
| UniProt ID | Q9BYJ9 |
| Cytogenetics | 20q13.33 |
| MW | 60.9 kDa |
| Summary | Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_017798" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207185L1 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), Myc-DDK-tagged | 10 ug |
$820.00
|
|
| RC207185L2 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), mGFP tagged | 10 ug |
$820.00
|
|
| RC207185L3 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), Myc-DDK-tagged | 10 ug |
$820.00
|
|
| RC207185L4 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), mGFP tagged | 10 ug |
$820.00
|
|
| RG207185 | YTHDF1 (tGFP-tagged) - Human YTH domain family, member 1 (YTHDF1) | 10 ug |
$489.00
$720.00
|
|
| SC113954 | YTHDF1 (untagged)-Human YTH domain family, member 1 (YTHDF1) | 10 ug |
$521.00
|
|
Citations
Targeting YTHDF1 effectively re-sensitizes cisplatin-resistant colon cancer cells by modulating GLS-mediated glutamine metabolism
Chen, Liu, Lin et al
Mol Ther Oncolytics (2021) 20, 228-239 DOI: 10.1016/j.omto.2021.01.001
Chen, Liu, Lin et al
Mol Ther Oncolytics (2021) 20, 228-239 DOI: 10.1016/j.omto.2021.01.001
Cited by 133
AI Summary: In this study, OriGene products including the YTHDF1 shRNA (Cat# TL320003) and GLS1 cDNA clone (Cat# RC209865) were used to manipulate gene expression and investigate the roles of YTHDF1 and GLS1 in modulating cisplatin resistance and glutamine metabolism in colon cancer cells.
YTHDF1 Aggravates the Progression of Cervical Cancer Through m6A-Mediated Up-Regulation of RANBP2
Wang, Luo, Kang et al
Front Oncol (2021) 11, 650383 DOI: 10.3389/fonc.2021.650383
Wang, Luo, Kang et al
Front Oncol (2021) 11, 650383 DOI: 10.3389/fonc.2021.650383
Cited by 79
AI Summary: In this study, OriGene products including shRNA lentiviral particles (catalog # TL320708) and YTHDF1 overexpression plasmid (catalog # RC221512) were used to modulate YTHDF1 expression in cervical cancer cells, enabling the investigation of its role in cancer progression.
microRNA-421-3p prevents inflammatory response in cerebral ischemia/reperfusion injury through targeting m6A Reader YTHDF1 to inhibit p65 mRNA translation
Zheng, Tang, Lu et al
Int Immunopharmacol (2020) 88, 106937 DOI: 10.1016/j.intimp.2020.106937
Zheng, Tang, Lu et al
Int Immunopharmacol (2020) 88, 106937 DOI: 10.1016/j.intimp.2020.106937
Cited by 102
AI Summary: OriGene products, specifically the YTHDF1 plasmid (Cat# RC212847) and YTHDF1 siRNA (Cat# SR312847), were used in this study to manipulate YTHDF1 expression and investigate its role as a target of microRNA-421-3p in regulating inflammatory responses during cerebral ischemia/reperfusion injury.
The m6A reader YTHDF1 promotes ovarian cancer progression via augmenting EIF3C translation
Liu, Wei, Jin et al
Nucleic Acids Res. (2020) 48 (7), 3816-3831 DOI: 10.1093/nar/gkaa048
Liu, Wei, Jin et al
Nucleic Acids Res. (2020) 48 (7), 3816-3831 DOI: 10.1093/nar/gkaa048
Cited by 818
Cap-independent mRNA translation is upregulated in long-lived endocrine mutant mice
Ozkurede, Kala, Johnson et al
J. Mol. Endocrinol. (2019) 63 (2), 123-138 DOI: 10.1530/JME-19-0021
Ozkurede, Kala, Johnson et al
J. Mol. Endocrinol. (2019) 63 (2), 123-138 DOI: 10.1530/JME-19-0021
Showing 1-5 of 5 papers.
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