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SLC44A2 (NM_020428) Human Tagged ORF Clone

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SKU
RC207181
SLC44A2 (Myc-DDK-tagged)-Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $646.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC44A2
Synonyms CTL2; PP1292
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207181 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGACGAGCGGCCCCACTACTACGGGAAACACGGAACGCCACAGAAGTATGATCCCACTTTCAAAG
GACCCATTTACAATAGGGGCTGCACGGATATCATATGCTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA
CGTGGCTGTAGGCATCATAGCCTGGACTCATGGAGACCCTCGAAAGGTGATCTACCCCACTGATAGCCGG
GGCGAGTTCTGCGGGCAGAAGGGCACAAAAAACGAGAACAAACCCTATCTGTTTTATTTCAACATTGTGA
AATGTGCCAGCCCCCTGGTTCTGCTGGAATTCCAATGTCCCACTCCCCAGATCTGCGTGGAAAAATGCCC
CGACCGCTACCTCACGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAGTACTATAAGCAGTTCTGTGTT
CCTGGCTTCAAGAACAATAAAGGAGTGGCTGAGGTGCTTCGAGATGGTGACTGCCCTGCTGTCCTCATCC
CCAGCAAACCCTTGGCCCGGAGATGCTTCCCCGCTATCCACGCCTACAAGGGTGTCCTGATGGTGGGCAA
TGAGACGACCTATGAGGATGGGCATGGCTCCCGGAAAAACATCACAGACCTGGTGGAGGGCGCCAAGAAA
GCCAATGGAGTCCTAGAGGCGCGGCAACTCGCCATGCGCATATTTGAAGATTACACCGTCTCTTGGTACT
GGATTATCATAGGCCTGGTCATTGCCATGGCGATGAGCCTCCTGTTCATCATCCTGCTTCGCTTCCTGGC
TGGTATTATGGTCTGGGTGATGATCATCATGGTGATTCTGGTGCTGGGCTACGGAATATTTCACTGCTAC
ATGGAGTACTCCCGACTGCGTGGTGAGGCCGGCTCTGATGTCTCTTTGGTGGACCTCGGCTTTCAGACGG
ATTTCCGGGTGTACCTGCACTTACGGCAGACCTGGTTGGCCTTTATGATCATTCTGAGTATCCTTGAAGT
CATTATCATCTTGCTGCTCATCTTTCTCCGGAAGAGAATTCTCATCGCGATTGCACTCATCAAAGAAGCC
AGCAGGGCTGTGGGATACGTCATGTGCTCCTTGCTCTACCCACTGGTCACCTTCTTCTTGCTGTGCCTCT
GCATCGCCTACTGGGCCAGCACTGCTGTCTTCCTGTCCACTTCCAACGAAGCGGTCTATAAGATCTTTGA
TGACAGCCCCTGCCCATTTACTGCGAAAACCTGCAACCCAGAGACCTTCCCCTCCTCCAATGAGTCCCGC
CAATGCCCCAATGCCCGTTGCCAGTTCGCCTTCTACGGTGGTGAGTCGGGCTACCACCGGGCCCTGCTGG
GCCTGCAGATCTTCAATGCCTTCATGTTCTTCTGGTTGGCCAACTTCGTGCTGGCGCTGGGCCAGGTCAC
GCTGGCCGGGGCCTTTGCCTCCTACTACTGGGCCCTGCGCAAGCCGGACGACCTGCCGGCCTTCCCGCTC
TTCTCTGCCTTTGGCCGGGCGCTCAGGTACCACACAGGCTCCCTGGCCTTTGGCGCGCTCATCCTGGCCA
TTGTGCAGATCATCCGTGTGATACTCGAGTACCTGGATCAGCGGCTGAAAGCTGCAGAGAACAAGTTTGC
CAAGTGCCTCATGACCTGTCTCAAATGCTGCTTCTGGTGCCTGGAGAAGTTCATCAAATTCCTTAATAGG
AATGCCTACATCATGATTGCCATCTACGGCACCAATTTCTGCACCTCGGCCAGGAATGCCTTCTTCCTGC
TCATGAGAAACATCATCAGAGTGGCTGTCCTGGATAAAGTTACTGACTTCCTCTTCCTGTTGGGCAAACT
TCTGATCGTTGGTAGTGTGGGGATCCTGGCTTTCTTCTTCTTCACCCACCGTATCAGGATCGTGCAGGAT
ACAGCACCACCCCTCAATTATTACTGGGTTCCTATACTGACGGTGATCGTTGGCTCCTACTTGATTGCAC
ACGGTTTCTTCAGCGTCTATGGCATGTGTGTGGACACGCTGTTCCTCTGCTTCTTGGAGGACCTGGAGAG
GAATGACGGCTCGGCCGAGAGGCCTTACTTCATGTCTTCCACCCTCAAGAAACCCTTGAACAAGACCAAC
AAGAAGGCAGCGGAGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207181 protein sequence
Red=Cloning site Green=Tags(s)

MGDERPHYYGKHGTPQKYDPTFKGPIYNRGCTDIICCVFLLLAIVGYVAVGIIAWTHGDPRKVIYPTDSR
GEFCGQKGTKNENKPYLFYFNIVKCASPLVLLEFQCPTPQICVEKCPDRYLTYLNARSSRDFEYYKQFCV
PGFKNNKGVAEVLRDGDCPAVLIPSKPLARRCFPAIHAYKGVLMVGNETTYEDGHGSRKNITDLVEGAKK
ANGVLEARQLAMRIFEDYTVSWYWIIIGLVIAMAMSLLFIILLRFLAGIMVWVMIIMVILVLGYGIFHCY
MEYSRLRGEAGSDVSLVDLGFQTDFRVYLHLRQTWLAFMIILSILEVIIILLLIFLRKRILIAIALIKEA
SRAVGYVMCSLLYPLVTFFLLCLCIAYWASTAVFLSTSNEAVYKIFDDSPCPFTAKTCNPETFPSSNESR
QCPNARCQFAFYGGESGYHRALLGLQIFNAFMFFWLANFVLALGQVTLAGAFASYYWALRKPDDLPAFPL
FSAFGRALRYHTGSLAFGALILAIVQIIRVILEYLDQRLKAAENKFAKCLMTCLKCCFWCLEKFIKFLNR
NAYIMIAIYGTNFCTSARNAFFLLMRNIIRVAVLDKVTDFLFLLGKLLIVGSVGILAFFFFTHRIRIVQD
TAPPLNYYWVPILTVIVGSYLIAHGFFSVYGMCVDTLFLCFLEDLERNDGSAERPYFMSSTLKKPLNKTN
KKAAES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020428
ORF Size 2118 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020428.2, NP_065161.2
RefSeq Size 3434 bp
RefSeq ORF 2121 bp
Locus ID 57153
UniProt ID Q8IWA5
Cytogenetics 19p13.2
Domains DUF580
Protein Families Transmembrane
MW 80.1 kDa
Summary Isoform 1, but not isoform 3, exhibits some choline transporter activity.UniProtKB/Swiss-Prot Function
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Documents
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"NM_020428" in other vectors (6)
SKU Description Size Price
RC207181L1 Lenti ORF clone of Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1, Myc-DDK-tagged 10 ug
$946.00
RC207181L2 Lenti ORF clone of Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1, mGFP tagged 10 ug
$946.00
RC207181L3 Lenti ORF clone of Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1, Myc-DDK-tagged 10 ug
$946.00
RC207181L4 Lenti ORF clone of Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1, mGFP tagged 10 ug
$946.00
RG207181 SLC44A2 (tGFP-tagged) - Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1 10 ug
$789.00 $846.00
SC127368 SLC44A2 (untagged)-Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1 10 ug
$647.00

Plasmid Map for RC207181

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207181
Circular map for RC207181

file-alt Citations

New age-related hearing loss candidate genes in humans: an ongoing challenge
Di Stazio, Morgan, Brumat et al
Gene (2020) 742, 144561 DOI: 10.1016/j.gene.2020.144561
Cited by 15
AI Summary: OriGene’s pCMV6-Entry vectors containing wild-type and mutant cDNAs of candidate genes (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3, and Strn) were used for transfection and protein expression studies to functionally assess gene variants associated with age-related hearing loss in this paper.
Snippet: The WT and human mutant genes of interest (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3 and Strn) cDNAs were cloned into a pCMV6-Entry vector (by Origene, Rockville, MD), Myc tagged, respectively. Vectors were purified through the PureLink HiPure Plasmid Maxiprep kit (Invitrogen) for transfection and protein expression
Showing 1-1 of 1 papers.
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