BATF (NM_006399) Human Tagged ORF Clone

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SKU
RC207104
BATF (Myc-DDK-tagged)-Human basic leucine zipper transcription factor, ATF-like (BATF)
  $225.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BATF
Synonyms B-ATF; BATF1; SFA-2; SFA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207104 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCACAGCTCCGACAGCAGTGACTCCAGCTTCAGCCGCTCTCCTCCCCCTGGCAAACAGGACTCAT
CTGATGATGTGAGAAGAGTTCAGAGGAGGGAGAAAAATCGTATTGCCGCCCAGAAGAGCCGACAGAGGCA
GACACAGAAGGCCGACACCCTGCACCTGGAGAGCGAAGACCTGGAGAAACAGAACGCGGCTCTACGCAAG
GAGATCAAGCAGCTCACAGAGGAACTGAAGTACTTCACGTCGGTGCTGAACAGCCACGAGCCCCTGTGCT
CGGTGCTGGCCGCCAGCACGCCCTCGCCCCCCGAGGTGGTGTACAGCGCCCACGCATTCCACCAACCTCA
TGTCAGCTCCCCGCGCTTCCAGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207104 protein sequence
Red=Cloning site Green=Tags(s)

MPHSSDSSDSSFSRSPPPGKQDSSDDVRRVQRREKNRIAAQKSRQRQTQKADTLHLESEDLEKQNAALRK
EIKQLTEELKYFTSVLNSHEPLCSVLAASTPSPPEVVYSAHAFHQPHVSSPRFQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006399
ORF Size 375 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006399.5
RefSeq Size 953 bp
RefSeq ORF 378 bp
Locus ID 10538
UniProt ID Q16520
Cytogenetics 14q24.3
Protein Families Transcription Factors
MW 14.1 kDa
Summary The protein encoded by this gene is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006399" in other vectors (6)
SKU Description Size Price
RC207104L1 Lenti ORF clone of Human basic leucine zipper transcription factor, ATF-like (BATF), Myc-DDK-tagged 10 ug
$525.00
RC207104L2 Lenti ORF clone of Human basic leucine zipper transcription factor, ATF-like (BATF), mGFP tagged 10 ug
$525.00
RC207104L3 Lenti ORF clone of Human basic leucine zipper transcription factor, ATF-like (BATF), Myc-DDK-tagged 10 ug
$525.00
RC207104L4 Lenti ORF clone of Human basic leucine zipper transcription factor, ATF-like (BATF), mGFP tagged 10 ug
$525.00
RG207104 BATF (tGFP-tagged) - Human basic leucine zipper transcription factor, ATF-like (BATF) 10 ug
$425.00
SC122745 BATF (untagged)-Human basic leucine zipper transcription factor, ATF-like (BATF) 10 ug
$225.00

Plasmid Map for RC207104

Download sequence for

Cloning scheme for RC207104

Circular map for RC207104

file-alt Citations

IRF4 haploinsufficiency in a family with Whipple's disease
Guérin, Kerner, Marr et al
Elife (2018) 7 DOI: 10.7554/eLife.32340
AI Summary: In this study, OriGene products BATF (RC207104) and JUN (RC209804) cDNA clones were used to investigate the molecular mechanisms underlying IRF4 haploinsufficiency in Whipple's disease.
Snippet: ermo Fisher Scientific) Gene (Human) PU.1 (NM_001080547.1) This paper Vector backbone: pcDNA 3.1D/V5-His-TOPO vector (Thermo Fisher Scientific) Gene (Human) BATF (NM_006399.3) OriGene RC207104 Gene (Human) JUN (NM_002228.3) OriGene RC209804 Strain (Tropheryma whipplei), strain background (DIG APD 25) Tw This paper NCBI taxon: 2039 Obtained fr;... ermo Fisher Scientific) Gene (Human) PU.1 (NM_001080547.1) This paper Vector backbone: pcDNA 3.1D/V5-His-TOPO vector (Thermo Fisher Scientific) Gene (Human) BATF (NM_006399.3) OriGene RC207104 Gene (Human) JUN (NM_002228.3) OriGene RC209804 Strain (Tropheryma whipplei), strain background (DIG APD 25) Tw This paper NCBI taxon: 2039 Obtained fr;The full-length cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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