OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

MIER2 (NM_017550) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC207065
MIER2 (Myc-DDK-tagged)-Human mesoderm induction early response 1, family member 2 (MIER2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $507.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA504260 MIER2 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7) 100 ul
$447.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "MIER2 or NM_017550"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MIER2
Synonyms KIAA1193; Mi-er2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207065 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCCTCCTCGCTGGGGAGGCAGAGTCCTCGCGTGGTCTCCTGCCTCGAGCACAGCCTGTGCC
CAGGGGAGCCGGGCTTGCAGACAACAGCAGTGGTGTCCATGGGCTCTGGAGACCATCAGTTCAACCTCGC
AGAGATCCTGTCACAGAACTACAGTGTTAGGGGGGAGTGCGAGGAGGCCTCGAGGTGCCCAGACAAGCCC
AAGGAGGAGCTGGAGAAGGACTTCATCTCCCAGAGCAACGACATGCCCTTTGATGAGCTGCTTGCGCTCT
ATGGCTACGAGGCGTCAGACCCCATTTCAGACCGGGAGAGTGAGGGTGGTGACGTGGCCCCGAACCTCCC
AGACATGACCCTGGACAAAGAACAAATAGCGAAGGATTTGCTTTCAGGGGAAGAAGAGGAAGAGACGCAA
TCATCTGCTGACGACCTCACCCCGTCCGTGACCTCCCACGAGGCCTCCGACCTCTTCCCTAACCGGAGTG
GATCTCGTTTCCTGGCTGATGAAGACAGAGAGCCTGGCTCTTCTGCCTCCTCCGACACCGAGGAGGACTC
TCTTCCTGCCAACAAATGTAAGAAGGAGATCATGGTGGGACCTCAGTTCCAAGCTGACCTCAGCAACCTG
CACTTGAACCGGCACTGTGAGAAGATCTACGAGAACGAAGACCAGCTGCTCTGGGACCCCAGCGTCCTCC
CTGAGAGGGAGGTGGAGGAGTTCCTGTACAGGGCGGTGAAGCGGCGTTGGCACGAGATGGCCGGGCCTCA
GCTCCCAGAGGGAGAAGCCGTGAAAGACAGTGAGCAGGCGCTGTACGAGTTGGTGAAATGCAACTTCAAT
GTGGAGGAGGCCCTGCGAAGGCTGCGGTTCAACGTGAAGGTGATCCGAGATGGGCTCTGTGCTTGGAGTG
AAGAGGAGTGCAGGAACTTTGAGCACGGCTTCCGTGTGCATGGAAAGAACTTTCACCTGATCCAGGCCAA
CAAGGTGCGCACACGGTCAGTGGGCGAGTGTGTCGAGTACTACTACCTGTGGAAGAAGTCGGAGCGCTAC
GACTACTTCGCCCAGCAGACGCGGCTGGGCCGGAGGAAGTACGTCCCGTCCGGAACCACGGACGCAGACC
AGGACCTGGATGGCAGCGACCCCGATGGCCCCGGCCGTCCGCGCCCGGAGCAAGACACCCTGACTGGGAT
GCGCACAGATCCACTGAGCGTGGATGGCACGGCCGGTGGTCTCGATGAGCCCGGAGTGGCCTCTGATGGA
CTCCCGTCCTCGGAGCCAGGGCCGTGTTCCTTCCAGCAGCTGGATGAGTCCCCCGCTGTACCCCTGTCCC
ATCGGCCCCCAGCCCTGGCCGACCCAGCCTCATACCAGCCAGCTGTCACTGCTCCGGAGCCAGACGCCAG
CCCAAGGCTGGCCGTGGACTTCGCCCTGCCCAAGGAGCTGCCCCTCATCTCCAGCCATGTGGACCTCAGC
GGGGATCCGGAGGAGACTGTGGCCCCAGCACAGGTGGCTTTGTCGGTCACCGAGTTTGGACTCATCGGCA
TTGGGGACGTGAACCCCTTCCTGGCCGCCCACCCCACGTGCCCGGCCCCCGGGCTACACTCGGAGCCCCT
GTCACACTGTAACGTGATGACCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207065 protein sequence
Red=Cloning site Green=Tags(s)

MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKP
KEELEKDFISQSNDMPFDELLALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQ
SSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSASSDTEEDSLPANKCKKEIMVGPQFQADLSNL
HLNRHCEKIYENEDQLLWDPSVLPEREVEEFLYRAVKRRWHEMAGPQLPEGEAVKDSEQALYELVKCNFN
VEEALRRLRFNVKVIRDGLCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERY
DYFAQQTRLGRRKYVPSGTTDADQDLDGSDPDGPGRPRPEQDTLTGMRTDPLSVDGTAGGLDEPGVASDG
LPSSEPGPCSFQQLDESPAVPLSHRPPALADPASYQPAVTAPEPDASPRLAVDFALPKELPLISSHVDLS
GDPEETVAPAQVALSVTEFGLIGIGDVNPFLAAHPTCPAPGLHSEPLSHCNVMTC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017550
ORF Size 1635 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017550.2
RefSeq Size 2825 bp
RefSeq ORF 1638 bp
Locus ID 54531
UniProt ID Q8N344
Cytogenetics 19p13.3
MW 59.9 kDa
Summary Transcriptional repressor.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_017550" in other vectors (4)
SKU Description Size Price
RC207065L3 Lenti ORF clone of Human mesoderm induction early response 1, family member 2 (MIER2), Myc-DDK-tagged 10 ug
$807.00
RC207065L4 Lenti ORF clone of Human mesoderm induction early response 1, family member 2 (MIER2), mGFP tagged 10 ug
$807.00
RG207065 MIER2 (tGFP-tagged) - Human mesoderm induction early response 1, family member 2 (MIER2) 10 ug
$489.00 $707.00
SC304479 MIER2 (untagged)-Human mesoderm induction early response 1, family member 2 (MIER2) 10 ug
$559.00

Plasmid Map for RC207065

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207065
Circular map for RC207065

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products