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MPP2 (NM_005374) Human Tagged ORF Clone

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SKU
RC207011
MPP2 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $514.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MPP2
Synonyms DLG2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207011 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGTTGCCGCCACCAACTCTGAAACTGCCATGCAGCAAGTCCTGGACAACTTGGGATCCCTCCCCA
GTGCCACGGGGGCTGCAGAGCTGGACCTGATCTTCCTTCGAGGCATTATGGAAAGTCCCATAGTAAGATC
CCTGGCCAAGGCCCATGAGAGGCTGGAGGAGACGAAGCTGGAGGCCGTGAGAGACAACAACCTGGAGCTG
GTGCAGGAGATCCTGCGGGACCTGGCGCAGCTGGCTGAGCAGAGCAGCACAGCCGCCGAGCTGGCCCACA
TCCTCCAGGAGCCCCACTTCCAGTCCCTCCTGGAGACGCACGACTCTGTGGCCTCAAAGACCTATGAGAC
ACCACCCCCCAGCCCTGGCCTGGACCCTACGTTCAGCAACCAGCCTGTACCTCCCGATGCTGTGCGCATG
GTGGGCATCCGCAAGACAGCCGGAGAACATCTGGGTGTAACGTTCCGCGTGGAGGGCGGCGAGCTGGTGA
TCGCGCGCATTCTGCATGGGGGCATGGTGGCTCAGCAAGGCCTGCTGCATGTGGGTGACATCATCAAGGA
GGTGAACGGGCAGCCAGTGGGCAGTGACCCCCGCGCACTGCAGGAGCTCCTGCGCAATGCCAGTGGCAGT
GTCATCCTCAAGATCCTGCCCAGCTACCAGGAGCCCCATCTGCCCCGCCAGGTATTTGTGAAATGTCACT
TTGACTATGACCCGGCCCGAGACAGCCTCATCCCCTGCAAGGAAGCAGGCCTGCGCTTCAACGCCGGGGA
CTTGCTCCAGATCGTAAACCAGGATGATGCCAACTGGTGGCAGGCATGCCATGTCGAAGGGGGCAGTGCT
GGGCTCATTCCCAGCCAGCTGCTGGAGGAGAAGCGGAAAGCATTTGTCAAGAGGGACCTGGAGCTGACAC
CAAACTCAGGGACCCTATGCGGCAGCCTTTCAGGAAAGAAAAAGAAGCGAATGATGTATTTGACCACCAA
GAATGCAGAGTTTGACCGTCATGAGCTGCTCATTTATGAGGAGGTGGCCCGCATGCCCCCGTTCCGCCGG
AAAACCCTGGTACTGATTGGGGCTCAGGGCGTGGGACGGCGCAGCCTGAAGAACAAGCTCATCATGTGGG
ATCCAGATCGCTATGGCACCACGGTGCCCTACACCTCCCGGCGGCCGAAAGACTCAGAGCGGGAAGGTCA
GGGTTACAGCTTTGTGTCCCGTGGGGAGATGGAGGCTGACGTCCGTGCTGGGCGCTACCTGGAGCATGGC
GAATACGAGGGCAACCTGTATGGCACACGTATTGACTCCATCCGGGGCGTGGTCGCTGCTGGGAAGGTGT
GCGTGCTGGATGTCAACCCCCAGGCGGTGAAGGTGCTACGAACGGCCGAGTTTGTCCCTTACGTGGTGTT
CATCGAGGCCCCAGACTTCGAGACCCTGCGGGCCATGAACAGGGCTGCGCTGGAGAGTGGAATATCCACC
AAGCAGCTCACGGAGGCGGACCTGAGACGGACAGTGGAGGAGAGCAGCCGCATCCAGCGGGGCTACGGGC
ACTACTTTGACCTCTGCCTGGTCAATAGCAACCTGGAGAGGACCTTCCGCGAGCTCCAGACAGCCATGGA
GAAGCTACGGACAGAGCCCCAGTGGGTGCCTGTCAGCTGGGTGTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207011 protein sequence
Red=Cloning site Green=Tags(s)

MPVAATNSETAMQQVLDNLGSLPSATGAAELDLIFLRGIMESPIVRSLAKAHERLEETKLEAVRDNNLEL
VQEILRDLAQLAEQSSTAAELAHILQEPHFQSLLETHDSVASKTYETPPPSPGLDPTFSNQPVPPDAVRM
VGIRKTAGEHLGVTFRVEGGELVIARILHGGMVAQQGLLHVGDIIKEVNGQPVGSDPRALQELLRNASGS
VILKILPSYQEPHLPRQVFVKCHFDYDPARDSLIPCKEAGLRFNAGDLLQIVNQDDANWWQACHVEGGSA
GLIPSQLLEEKRKAFVKRDLELTPNSGTLCGSLSGKKKKRMMYLTTKNAEFDRHELLIYEEVARMPPFRR
KTLVLIGAQGVGRRSLKNKLIMWDPDRYGTTVPYTSRRPKDSEREGQGYSFVSRGEMEADVRAGRYLEHG
EYEGNLYGTRIDSIRGVVAAGKVCVLDVNPQAVKVLRTAEFVPYVVFIEAPDFETLRAMNRAALESGIST
KQLTEADLRRTVEESSRIQRGYGHYFDLCLVNSNLERTFRELQTAMEKLRTEPQWVPVSWVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005374
ORF Size 1656 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005374.2
RefSeq Size 4645 bp
RefSeq ORF 1659 bp
Locus ID 4355
UniProt ID Q14168, D3DX48
Cytogenetics 17q21.31
Domains Guanylate_kin, GuKc, L27, PDZ, SH3
Protein Families Druggable Genome
MW 61.6 kDa
Summary Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005374" in other vectors (6)
SKU Description Size Price
RC207011L1 Lenti ORF clone of Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2), Myc-DDK-tagged 10 ug
$814.00
RC207011L2 Lenti ORF clone of Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2), mGFP tagged 10 ug
$814.00
RC207011L3 Lenti ORF clone of Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2), Myc-DDK-tagged 10 ug
$814.00
RC207011L4 Lenti ORF clone of Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2), mGFP tagged 10 ug
$814.00
RG207011 MPP2 (tGFP-tagged) - Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2) 10 ug
$489.00 $714.00
SC116778 MPP2 (untagged)-Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2) 10 ug
$515.00

Plasmid Map for RC207011

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207011
Circular map for RC207011

file-alt Citations

The Kv1-associated molecules TAG-1 and Caspr2 are selectively targeted to axon initial segment in hippocampal neurons
Pinatel, Hivert, Saint-Martin et al
J. Cell. Sci. (2017)
AI Summary: In this study, human CASK and MPP2 cDNA clones obtained from OriGene were used to investigate the molecular determinants regulating Caspr2 localization in hippocampal neurons.
Snippet: sites of pmCherry-N1. The coding sequence of human CASK and MPP2 were obtained from OriGene (Rockville, USA) and inserted into the EcoR1-BamH1 sites of pmCherry-N1 vector. PCR amplified products were verified by sequencing (Genewiz, Takeley, ... All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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