Rel B (RELB) (NM_006509) Human Tagged ORF Clone
SKU
RC207006
RELB (Myc-DDK-tagged)-Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB)
$539.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Rel B |
| Synonyms | I-REL; IMD53; IREL; REL-B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006509 |
| ORF Size | 1737 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006509.4 |
| RefSeq Size | 2287 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 5971 |
| UniProt ID | Q01201 |
| Cytogenetics | 19q13.32 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | MAPK signaling pathway |
| MW | 62 kDa |
| Summary | NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function (PubMed:26385063).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006509" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207006L1 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), Myc-DDK-tagged | 10 ug |
$839.00
|
|
| RC207006L2 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), mGFP tagged | 10 ug |
$839.00
|
|
| RC207006L3 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), Myc-DDK-tagged | 10 ug |
$839.00
|
|
| RC207006L4 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), mGFP tagged | 10 ug |
$839.00
|
|
| RG207006 | RELB (tGFP-tagged) - Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB) | 10 ug |
$489.00
$739.00
|
|
| SC122747 | RELB (untagged)-Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB) | 10 ug |
$540.00
|
|
Citations
Inherited human RelB deficiency impairs innate and adaptive immunity to infection
Le Voyer, Maglorius Renkilaraj, Moriya et al
Proc Natl Acad Sci U S A (2024) 121 (37), e2321794121 DOI: 10.1073/pnas.2321794121
Le Voyer, Maglorius Renkilaraj, Moriya et al
Proc Natl Acad Sci U S A (2024) 121 (37), e2321794121 DOI: 10.1073/pnas.2321794121
Cited by 13
AI Summary: OriGene’s pCMV6 vector encoding C-terminally Flag-tagged human RelB was used to express wild-type and mutant RELB in HEK293T cells, enabling functional studies of RELB variants in this investigation of RelB deficiency and immune dysfunction.
Snippet: ome Analyzer IIx (Illumina), generating 72-base reads. Transfection of HEK293T Cells and Expression of RELB Variants. The pCMV6 vector encoding C-terminally Flag-tagged human RelB was purchased from Origene (Maryland, USA). The construct carrying the mutant allele was generated by direct mutagenesis with the CloneAmp HIFI PCR Premix (Takara, Japan). Cells were transfected for 24 h, after which Re
Glycogen synthase kinase-3ß is a crucial mediator of signal-induced RelB degradation
Neumann, Klar, Wilisch-Neumann et al
Oncogene (2011)
Neumann, Klar, Wilisch-Neumann et al
Oncogene (2011)
The RelB alternative NF-kappaB subunit promotes autophagy in 22Rv1 prostate cancer cells in vitro and affects mouse xenograft tumor growth in vivo
Labouba, Poisson, Lafontaine et al
cancer cell microenvironment (2014)
Labouba, Poisson, Lafontaine et al
cancer cell microenvironment (2014)
AI Summary: OriGene's RelB (NM_006509) cDNA was used to generate lentiviral vectors for overexpression studies in 22Rv1 prostate cancer cells, enabling the investigation of RelB’s role in autophagy and tumor growth.
Snippet: of puromycin (Sigma, St. Louis, MO). Lentiviral production and transduction RelB (NM_006509, from OriGene, Rockville, MD, USA) was inserted in pENTR/D-TOPO (Invitrogen, NY, USA). The generated pENTR-RelB vector ... Cite SaveSaving
Showing 1-3 of 3 papers.
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