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B3GNT5 (NM_032047) Human Tagged ORF Clone

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SKU
RC206965
B3GNT5 (Myc-DDK-tagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "B3GNT5 or NM_032047"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol B3GNT5
Synonyms B3GN-T5; beta3Gn-T5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206965 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAATGTTGGTTAGTGGCAGAAGAGTCAAAAAATGGCAGTTAATTATTCAGTTATTTGCTACTTGTT
TTTTAGCGAGCCTCATGTTTTTTTGGGAACCAATCGATAATCACATTGTGAGCCATATGAAGTCATATTC
TTACAGATACCTCATAAATAGCTATGACTTTGTGAATGATACCCTGTCTCTTAAGCACACCTCAGCGGGG
CCTCGCTACCAATACTTGATTAACCACAAGGAAAAGTGTCAAGCTCAAGACGTCCTCCTTTTACTGTTTG
TAAAAACTGCTCCTGAAAACTATGATCGACGTTCCGGAATTAGAAGGACGTGGGGCAATGAAAATTATGT
TCGGTCTCAGCTGAATGCCAACATCAAAACTCTGTTTGCCTTAGGAACTCCTAATCCACTGGAGGGAGAA
GAACTACAAAGAAAACTGGCTTGGGAAGATCAAAGGTACAATGATATAATTCAGCAAGACTTTGTTGATT
CTTTCTACAATCTTACTCTGAAATTACTTATGCAGTTCAGTTGGGCAAATACCTATTGTCCACATGCCAA
ATTTCTTATGACTGCTGATGATGACATATTTATTCACATGCCAAATCTGATTGAGTACCTTCAAAGTTTA
GAACAAATTGGTGTTCAAGACTTTTGGATTGGTCGTGTTCATCGTGGTGCCCCTCCCATTAGAGATAAAA
GCAGCAAATACTACGTGTCCTATGAAATGTACCAGTGGCCAGCTTACCCTGACTACACAGCCGGAGCTGC
CTATGTAATCTCCGGTGATGTAGCTGCCAAAGTCTATGAGGCATCACAGACACTAAATTCAAGTCTTTAC
ATAGACGATGTGTTCATGGGCCTCTGTGCCAATAAAATAGGGATAGTACCGCAGGACCATGTGTTTTTTT
CTGGAGAGGGTAAAACTCCTTATCATCCCTGCATCTATGAAAAAATGATGACATCTCATGGACACTTAGA
AGATCTCCAGGACCTTTGGAAGAATGCTACAGATCCTAAAGTAAAAACCATTTCCAAAGGTTTTTTTGGT
CAAATATACTGCAGATTAATGAAGATAATTCTCCTTTGTAAAATTAGCTATGTGGACACATACCCTTGTA
GGGCTGCGTTTATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206965 protein sequence
Red=Cloning site Green=Tags(s)

MRMLVSGRRVKKWQLIIQLFATCFLASLMFFWEPIDNHIVSHMKSYSYRYLINSYDFVNDTLSLKHTSAG
PRYQYLINHKEKCQAQDVLLLLFVKTAPENYDRRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGE
ELQRKLAWEDQRYNDIIQQDFVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSL
EQIGVQDFWIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLY
IDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKVKTISKGFFG
QIYCRLMKIILLCKISYVDTYPCRAAFI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032047
ORF Size 1134 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032047.5
RefSeq Size 4131 bp
RefSeq ORF 1137 bp
Locus ID 84002
UniProt ID Q9BYG0
Cytogenetics 3q27.1
Domains Galactosyl_T
Protein Families Transmembrane
Protein Pathways Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
MW 44.1 kDa
Summary This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. provided by RefSeq, Jul 2008
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"NM_032047" in other vectors (7)
SKU Description Size Price
RC206965L1 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), Myc-DDK-tagged 10 ug
$757.00
RC206965L2 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), mGFP tagged 10 ug
$757.00
RC206965L3 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), Myc-DDK-tagged 10 ug
$757.00
RC206965L4 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), mGFP tagged 10 ug
$757.00
RG206965 B3GNT5 (tGFP-tagged) - Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) 10 ug
$489.00 $657.00
SC106413 B3GNT5 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) 10 ug
$457.00
SC323946 B3GNT5 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) 10 ug
$457.00

Plasmid Map for RC206965

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206965
Circular map for RC206965

file-alt Citations

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