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B3GNT5 (NM_032047) Human Untagged Clone

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SKU
SC106413
B3GNT5 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol B3GNT5
Synonyms B3GN-T5; beta3Gn-T5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC106413 sequence for NM_032047 edited (data generated by NextGen Sequencing)
ATGAGAATGTTGGTTAGTGGCAGAAGAGTCAAAAAATGGCAGTTAATTATTCAGTTATTT
GCTACTTGTTTTTTAGCGAGCCTCATGTTTTTTTGGGAACCAATCGATAATCACATTGTG
AGCCATATGAAGTCATATTCTTACAGATACCTCATAAATAGCTATGACTTTGTGAATGAT
ACCCTGTCTCTTAAGCACACCTCAGCGGGGCCTCGCTACCAATACTTGATTAACCACAAG
GAAAAGTGTCAAGCTCAAGACGTCCTCCTTTTACTGTTTGTAAAAACTGCTCCTGAAAAC
TATGATCGACGTTCCGGAATTAGAAGGACGTGGGGCAATGAAAATTATGTTCGGTCTCAG
CTGAATGCCAACATCAAAACTCTGTTTGCCTTAGGAACTCCTAATCCACTGGAGGGAGAA
GAACTACAAAGAAAACTGGCTTGGGAAGATCAAAGGTACAATGATATAATTCAGCAAGAC
TTTGTTGATTCTTTCTACAATCTTACTCTGAAATTACTTATGCAGTTCAGTTGGGCAAAT
ACCTATTGTCCACATGCCAAATTTCTTATGACTGCTGATGATGACATATTTATTCACATG
CCAAATCTGATTGAGTACCTTCAAAGTTTAGAACAAATTGGTGTTCAAGACTTTTGGATT
GGTCGTGTTCATCGTGGTGCCCCTCCCATTAGAGATAAAAGCAGCAAATACTACGTGTCC
TATGAAATGTACCAGTGGCCAGCTTACCCTGACTACACAGCCGGAGCTGCCTATGTAATC
TCCGGTGATGTAGCTGCCAAAGTCTATGAGGCATCACAGACACTAAATTCAAGTCTTTAC
ATAGACGATGTGTTCATGGGCCTCTGTGCCAATAAAATAGGGATAGTACCGCAGGACCAT
GTGTTTTTTTCTGGAGAGGGTAAAACTCCTTATCATCCCTGCATCTATGAAAAAATGATG
ACATCTCATGGACACTTAGAAGATCTCCAGGACCTTTGGAAGAATGCTACAGATCCTAAA
GTAAAAACCATTTCCAAAGGTTTTTTTGGTCAAATATACTGCAGATTAATGAAGATAATT
CTCCTTTGTAAAATTAGCTATGTGGACACATACCCTTGTAGGGCTGCGTTTATCTAA

Clone variation with respect to NM_032047.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032047 unedited
NNNCCCGTTCGGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGG
GCCGGTGGTTCCGCGCGGAGTTAGTCTGTGGTCAGTTACGTGGTGAAAACACGGCTGTGC
CGCGGCCGCATCTTTCCGCGGCCGAGGCCTCTCTGGGTGGGAGTGTTGGCTTCCTTTCCG
GATCGCTAAATGGGGAAAGTTCTGGCCGCTCGGCGGGATACGTCTCCAGGCCACGGATGG
TTCGTTCTCCGTGCCGCGGCCCCGAGCTGGGCTCCCTGGGTCTCCAGCGCGGGCTCCCGG
CATTGGGGGCTGCGGGCCGGCCCCTCCGCCCCGCCCCCGCCCCGCCGCGCCTCCTCGGCC
GAGCGGCTCGCGGTCTCCGGCGCGGGAGGCTCCGAGTCTGCCCACTCCGGGCCGAGCGAG
GTCTCTGGAGGAGAAGAGTGGCGAGGAGGTGAGGGCACGCCGGCCCTCGCCCGGCGGGTG
GCGCCAGGACTTCAGGTGGCATGGAATATTCACATGGGAGAGCCGCATGAGGCCGCCCAC
CACGCTTCCTGAAGGATGCCCGTGTGGAAGAATTTTGACGTGCCAGTGTCCTCGTTCTAC
AGGGTGTTCCATTCTTCCGCAATCTCAGAAAAATGGGACTAANAGAAACTATTTTGTAAA
ATAAGAAGACTTCCATTTTTTATGACCAACATGTATTAAGATGGACACCTACTCTACGAA
ACACGAAGTTCTATGGTCTCGAAGAAGCCCGTGCCTGTTTAAAACTGNATCCTACTAANA
ACAGACTTGAGTGGATATGAAGATGNTGGTTAGTGGCAGAAGAGTCAAAAATGGCAGTTA
ATTATTCANGTATTTGCTACTTGTTTTTTAGCGAGCCTCATGTTTTTTTGGGAACCAATC
GATAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032047 unedited
NNNNTTACTCTGNNACCGCGGCCGCATTCTAGNGATCGGTTTTTTTTTTTTTTTTTTTAA
TTTTTCAGTTGTTTATTGGAAACTATAGATTTTTTTTAAACTCACTAATTATTTTGCCAT
ACAAGAGCAAAATTCAAGTAATACAAATATTTAACATTTTGGTATAAAATTAACAATATG
GAAAAAAGATGCAAACCAACCAAAAGGTAAAAAAATTTTAACAATAACACTGTGTATAAC
CATCTTCTATTCAAGTGACCATCCAAGCTGATAGTTTATTTTCCCTTTGTTGACATTTTT
TAAAAAGATAGCCCTACACTGGAGCACCAAAAAGTTTTTTCCTTCTTTTTTAAAAGGTTG
GAACCCTTTTATTTACAGATACTCTTCTCAGTACTTTAAGTAAGTGTGATGAAGGAATCA
TCTAGCAACTTCCTTCTTAGAAAAAAAGGAAGTGCCTTCATATTTCCTTGAAATTTAAAC
TTGTTCCATTCTATTCTAAGCAAAAATTAAAAGGACACAGTTCAGAAGAGCTCTTTCAGC
AAATAAATAATTGTTTCACAAAAGCACTGCTGTAAACAAGATCACTTTGATGGCCAGAGA
CACTTATGTTTTCAACCAATGGCAACCTTAAACACTTCCAAGTATAGATACACAGGGTAT
ATATGGGCAAAAGGGCATACATCATTAATCAATCAACTAATAAAAATTAATTATAAGCTT
GGTGCTTGGNCAAATATGCCTTTTGGTCCCTAAGTTTTTTTTAATTGGTCAGAAAACTTA
AACTGTAATGATCTAAAAACCTGTATCTACTTTTGAAAGTAACTACACCCTAAAATGGTT
GACACTTGTGAGTTTGGCATTAGTTTAAAATTTTTAATTATCTAAGGATTGTAAACAAAG
CCTAAATTTCAAAAAGAAAAAATTTAAATTTCGGAACCATAAAAA
Restriction Sites ECoRI-NOT |
ACCN NM_032047
Insert Size 4400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032047.3, NP_114436.1
RefSeq Size 4127 bp
RefSeq ORF 1137 bp
Locus ID 84002
UniProt ID Q9BYG0
Cytogenetics 3q27.1
Domains Galactosyl_T
Protein Families Transmembrane
Protein Pathways Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
Summary This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_032047" in other vectors (7)
SKU Description Size Price
RC206965 B3GNT5 (Myc-DDK-tagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) 10 ug
$457.00
RC206965L1 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), Myc-DDK-tagged 10 ug
$757.00
RC206965L2 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), mGFP tagged 10 ug
$757.00
RC206965L3 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), Myc-DDK-tagged 10 ug
$757.00
RC206965L4 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), mGFP tagged 10 ug
$757.00
RG206965 B3GNT5 (tGFP-tagged) - Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) 10 ug
$489.00 $657.00
SC323946 B3GNT5 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) 10 ug
$457.00

file-alt Citations

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