TMCC3 (NM_020698) Human Tagged ORF Clone

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SKU
RC206925
TMCC3 (Myc-DDK-tagged)-Human transmembrane and coiled-coil domain family 3 (TMCC3)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMCC3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206925 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGCAGCGACACGGCGCTCACCGTGGACCGGACCTACTCGGACCCCGGCCGGCACCACCGCTGCA
AGAGCCGGGTAGAACGTCATGACATGAATACCTTAAGCCTGCCCCTGAACATACGCCGAGGGGGGTCAGA
CACCAACCTCAACTTTGATGTCCCGGATGGCATCCTGGACTTCCACAAGGTCAAACTCACTGCAGACAGC
CTGAAGCAAAAAATTCTAAAGGTAACAGAGCAGATAAAAATTGAGCAAACATCGCGCGATGGGAATGTTG
CGGAGTATCTGAAACTAGTGAACAACGCAGACAAGCAGCAGGCGGGACGTATCAAGCAAGTCTTTGAGAA
GAAGAATCAGAAATCAGCTCACTCCATCGCCCAGCTGCAGAAGAAGTTAGAGCAGTATCATCGAAAGCTC
AGAGAGATCGAGCAGAATGGAGCCTCTAGGAGCTCAAAGGACATTTCCAAAGACCACCTGAAGGATATAC
ATCGCTCTTTGAAAGATGCCCACGTGAAATCTCGAACTGCCCCCCATTGCATGGAGAGCAGCAAATCGGG
CATGCCAGGGGTCTCACTTACTCCACCTGTGTTCGTTTTCAATAAGTCCAGAGAGTTTGCCAACCTGATC
CGGAATAAGTTTGGCAGCGCCGACAACATTGCTCACTTGAAAAATTCCTTAGAGGAGTTTAGGCAAGAGG
CGAGTGCCAGGGCCTACGGGGGCAGCGCTACCATCGTGAACAAACCCAAGTATGGCAGTGATGATGAATG
TTCGAGTGGCACGTCAGGCTCGGCCGACAGTAACGGAAACCAGTCGTTTGGGGCTGGTGGAGCCAGCACA
CTGGACAGCCAGGGCAAGCTCGCCGTGATCCTGGAGGAACTGAGGGAGATCAAGGATACCCAAGCTCAGC
TGGCTGAGGACATCGAGGCACTGAAGGTGCAGTTTAAGAGAGAATATGGTTTTATTTCTCAGACCCTGCA
AGAGGAAAGATACAGGTATGAGCGACTGGAGGACCAGCTGCATGACCTGACGGACCTGCATCAGCATGAG
ACAGCCAACCTGAAGCAGGAGCTGGCCAGCATTGAGGAGAAGGTGGCCTACCAGGCCTACGAGCGCTCGC
GGGACATCCAGGAAGCCTTGGAATCCTGCCAGACTCGCATTTCTAAGCTGGAGCTCCACCAGCAAGAGCA
GCAAGCTCTGCAGACAGACACCGTGAATGCTAAAGTTCTCCTGGGGAGGTGCATCAACGTGATCCTGGCC
TTCATGACTGTCATCTTAGTGTGTGTGTCCACCATCGCGAAGTTCGTCTCACCCATGATGAAGAGTCGCT
GCCACATTCTTGGCACCTTCTTTGCCGTGACTCTTCTTGCTATATTTTGTAAAAACTGGGACCATATCCT
GTGTGCCATAGAAAGGATGATAATACCAAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206925 protein sequence
Red=Cloning site Green=Tags(s)

MPGSDTALTVDRTYSDPGRHHRCKSRVERHDMNTLSLPLNIRRGGSDTNLNFDVPDGILDFHKVKLTADS
LKQKILKVTEQIKIEQTSRDGNVAEYLKLVNNADKQQAGRIKQVFEKKNQKSAHSIAQLQKKLEQYHRKL
REIEQNGASRSSKDISKDHLKDIHRSLKDAHVKSRTAPHCMESSKSGMPGVSLTPPVFVFNKSREFANLI
RNKFGSADNIAHLKNSLEEFRQEASARAYGGSATIVNKPKYGSDDECSSGTSGSADSNGNQSFGAGGAST
LDSQGKLAVILEELREIKDTQAQLAEDIEALKVQFKREYGFISQTLQEERYRYERLEDQLHDLTDLHQHE
TANLKQELASIEEKVAYQAYERSRDIQEALESCQTRISKLELHQQEQQALQTDTVNAKVLLGRCINVILA
FMTVILVCVSTIAKFVSPMMKSRCHILGTFFAVTLLAIFCKNWDHILCAIERMIIPR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020698
ORF Size 1431 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020698.1, NP_065749.1
RefSeq Size 5881 bp
RefSeq ORF 1434 bp
Locus ID 57458
UniProt ID Q9ULS5
Cytogenetics 12q22
Protein Families Transmembrane
MW 53.8 kDa
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Documents
Resources
"NM_020698" in other vectors (6)
SKU Description Size Price
RC206925L1 Lenti ORF clone of Human transmembrane and coiled-coil domain family 3 (TMCC3), Myc-DDK-tagged 10 ug
$757.00
RC206925L2 Lenti ORF clone of Human transmembrane and coiled-coil domain family 3 (TMCC3), mGFP tagged 10 ug
$757.00
RC206925L3 Lenti ORF clone of Human transmembrane and coiled-coil domain family 3 (TMCC3), Myc-DDK-tagged 10 ug
$757.00
RC206925L4 Lenti ORF clone of Human transmembrane and coiled-coil domain family 3 (TMCC3), mGFP tagged 10 ug
$757.00
RG206925 TMCC3 (tGFP-tagged) - Human transmembrane and coiled-coil domain family 3 (TMCC3) 10 ug
$657.00
SC126820 TMCC3 (untagged)-Human transmembrane and coiled-coil domain family 3 (TMCC3) 10 ug
$457.00

Plasmid Map for RC206925

Download sequence for

Cloning scheme for RC206925

Circular map for RC206925

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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