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IHO1 (NM_178173) Human Tagged ORF Clone

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SKU
RC206914
CCDC36 (Myc-DDK-tagged)-Human coiled-coil domain containing 36 (CCDC36), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $544.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IHO1
Synonyms CCDC36; CT74; LELA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206914 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCAGTATTCCTTCAGGCTCTGGGAACAAGAAGTCATCCAACTGGAATAATAATCAAAATGATTATT
CCAGTCTCAGTGATTCCCAGTTCCTCTTTGGATCTCAGTTCTGTCCAGAAAATTCAGAAACCCTATCAGC
ACCCTTGGACTTTGGTGCCCACTTGAGACATTCAAAACAGTCACAACAGAACTATCTGGAGGGTGAACCT
AGCATTTTCACAAAGTACCAGACAAAGCCCCAGCTGTTCGGAGGAGATATAAAAGATGGAGGTTTATTTC
CTCCTCCTTTGTCAGTTGGAAAATCAAAAGGCCTCTTGGAACAGTTTGAGGAGAAAAAGAAAAGGGCAAA
AGACAAATGTGACAGTGAGACTCTATACAACTTTGTTTCTAATGTTAGAGAAAGCATTCTCAGGTTGCAG
ACGTCTGTGGAAAAGTCTGAGGACCATCTCAGTTCAAGAAGCCAATCTATTTTGGATTCTTTGGAGACTG
TGGCCAAGACATTACAAGAGACTATACAGGCCCAGAATGACCTGGTGTTTGAGGCAGTCCAGGACAAAGG
CAACATGGAGCAGGCCATCCTTGAGATGAAGAAAAGATTTGAAGCTAGACAAGGAGAGTTTATAGAAATG
AAGTCCAACCTGAAGCACCTTGAAGTTTTAGTTGCTCAGCAGAGTCAGGAATTCCAGCAGCTGTGTGAGC
AGCTAGGCCAGCTGAATGTGCCCAGTGTCCTAGCAGAGCTGAAGAGATTGATCTCAGTGCCTCCAGTGAA
AGACAGTGCTTCTCAGACGTCGCCACCTTTGGCCCAGAGCCTCAATCTCACCAGGCAGGAAAAATACACC
TCTGAGAAACCAGTTTTATGGCAGGCCCAGGCCCTCCCTGCTGCATGGAATCCTGGTATGGGCTCCCTAC
AGCCTGGAGAATTTGATGTCTGGGGTGAAGGAGCAAAGAATGATGATCTCCAAGAAGAGGCTGCACTGCC
AGCATTTGGGTCCCATGAAAGAAATAGGCATGTAAAGGACAAGGTGGTGCAGACTAACTGCAAGAACTGG
GCTGTTACTAAAACAGGTGCCAAGAACCATGGTTCCAGCGTCCCAGGCCATAAGATTCCCAGTGACAGGG
ACCTGGTTTCCCAAGGAGCCTCACAGCTCACATCATTGGAGATAAACTTTTCAACCAGCATTAAGAATGC
CTGCCAAAAATATCAAGCCCAAAGTATGTTTTTGTGTGACCCACGTGAACATTTGGTGATTAAACAGAAA
GATGGGACTGTAGAAATGCGGGGGAAAGACAAGAAGCAGCAGCCCAGGAAGGCCCACAGGGCCCACAGAG
GCAGGCTCATAGCCAGCAAGCAAAAACAAATCCCAATCCAGACCTGTAAATTCAATTCCAAATATCAGAG
TCCTCAGCCTGCAATTTCTGTCCCTCAAAGCCCCTTCCTGGGGCAGCAGGAACCCCGTGCTCAGCCTCTG
CATCTGCAGTGTCCCAGGAGCCCCAGAAAACCAGTCTGCCCTATTCTGGGAGGAACAGTCATGCCCAATA
AGACAGTAAGGGCAGTGCAGGGAAGACTCTTGCAGCTCAGCAGGTGCTCTTCCCAAGACAACTGGCTACT
TTCCAGCAGTTCCCAGGGGGACCACCAGATGAGCTGGTTCAGTGACCTCAATCTCGGATGTTCAGAGACC
CCTCTATGCAAGGAGGCAGGAAAGAATTTGCTCTATGACCTGGGTTTTGATAGCAGTGATGATGATGGCT
TC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206914 protein sequence
Red=Cloning site Green=Tags(s)

MLSIPSGSGNKKSSNWNNNQNDYSSLSDSQFLFGSQFCPENSETLSAPLDFGAHLRHSKQSQQNYLEGEP
SIFTKYQTKPQLFGGDIKDGGLFPPPLSVGKSKGLLEQFEEKKKRAKDKCDSETLYNFVSNVRESILRLQ
TSVEKSEDHLSSRSQSILDSLETVAKTLQETIQAQNDLVFEAVQDKGNMEQAILEMKKRFEARQGEFIEM
KSNLKHLEVLVAQQSQEFQQLCEQLGQLNVPSVLAELKRLISVPPVKDSASQTSPPLAQSLNLTRQEKYT
SEKPVLWQAQALPAAWNPGMGSLQPGEFDVWGEGAKNDDLQEEAALPAFGSHERNRHVKDKVVQTNCKNW
AVTKTGAKNHGSSVPGHKIPSDRDLVSQGASQLTSLEINFSTSIKNACQKYQAQSMFLCDPREHLVIKQK
DGTVEMRGKDKKQQPRKAHRAHRGRLIASKQKQIPIQTCKFNSKYQSPQPAISVPQSPFLGQQEPRAQPL
HLQCPRSPRKPVCPILGGTVMPNKTVRAVQGRLLQLSRCSSQDNWLLSSSSQGDHQMSWFSDLNLGCSET
PLCKEAGKNLLYDLGFDSSDDDGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178173
ORF Size 1752 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178173.2, NP_835467.1
RefSeq Size 2994 bp
RefSeq ORF 1785 bp
Locus ID 339834
UniProt ID Q8IYA8
Cytogenetics 3p21.31
MW 65.1 kDa
Summary Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.UniProtKB/Swiss-Prot Function
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"NM_178173" in other vectors (5)
SKU Description Size Price
RC206914L3 Lenti ORF clone of Human coiled-coil domain containing 36 (CCDC36), transcript variant 1, Myc-DDK-tagged 10 ug
$844.00
RC206914L4 Lenti ORF clone of Human coiled-coil domain containing 36 (CCDC36), transcript variant 1, mGFP tagged 10 ug
$844.00
RG206914 CCDC36 (tGFP-tagged) - Human coiled-coil domain containing 36 (CCDC36), transcript variant 1 10 ug
$489.00 $744.00
SC126356 CCDC36 (untagged)-Human coiled-coil domain containing 36 (CCDC36), transcript variant 1 10 ug
$545.00
SC327936 CCDC36 (untagged)-Human coiled-coil domain containing 36 (CCDC36) transcript variant 1 10 ug
$609.00

Plasmid Map for RC206914

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206914
Circular map for RC206914

file-alt Citations

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