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IHO1 (NM_178173) Human Untagged Clone

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SKU
SC327936
CCDC36 (untagged)-Human coiled-coil domain containing 36 (CCDC36) transcript variant 1
  $609.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IHO1
Synonyms CCDC36; CT74; LELA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC327936 representing NM_178173.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAATTTTAATGTCTGGAATATCAAAGAGATGCTCAGTATTCCTTCAGGCTCTGGGAACAAGAAGTCA
TCCAACTGGAATAATAATCAAAATGATTATTCCAGTCTCAGTGATTCCCAGTTCCTCTTTGGATCTCAG
TTCTGTCCAGAAAATTCAGAAACCCTATCAGCACCCTTGGACTTTGGTGCCCACTTGAGACATTCAAAA
CAGTCACAACAGAACTATCTGGAGGGTGAACCTAGCATTTTCACAAAGTACCAGACAAAGCCCCAGCTG
TTCGGAGGAGATATAAAAGATGGAGGTTTATTTCCTCCTCCTTTGTCAGTTGGAAAATCAAAAGGCCTC
TTGGAACAGTTTGAGGAGAAAAAGAAAAGGGCAAAAGACAAATGTGACAGTGAGACTCTATACAACTTT
GTTTCTAATGTTAGAGAAAGCATTCTCAGGTTGCAGACGTCTGTGGAAAAGTCTGAGGACCATCTCAGT
TCAAGAAGCCAATCTATTTTGGATTCTTTGGAGACTGTGGCCAAGACATTACAAGAGACTATACAGGCC
CAGAATGACCTGGTGTTTGAGGCAGTCCAGGACAAAGGCAACATGGAGCAGGCCATCCTTGAGATGAAG
AAAAGATTTGAAGCTAGACAAGGAGAGTTTATAGAAATGAAGTCCAACCTGAAGCACCTTGAAGTTTTA
GTTGCTCAGCAGAGTCAGGAATTCCAGCAGCTGTGTGAGCAGCTAGGCCAGCTGAATGTGCCCAGTGTC
CTAGCAGAGCTGAAGAGATTGATCTCAGTGCCTCCAGTGAAAGACAGTGCTTCTCAGACGTCGCCACCT
TTGGCCCAGAGCCTCAATCTCACCAGGCAGGAAAAATACACCTCTGAGAAACCAGTTTTATGGCAGGCC
CAGGCCCTCCCTGCTGCATGGAATCCTGGTATGGGCTCCCTACAGCCTGGAGAATTTGATGTCTGGGGT
GAAGGAGCAAAGAATGATGATCTCCAAGAAGAGGCTGCACTGCCAGCATTTGGGTCCCATGAAAGAAAT
AGGCATGTAAAGGACAAGGTGGTGCAGACTAACTGCAAGAACTGGGCTGTTACTAAAACAGGTGCCAAG
AACCATGGTTCCAGCGTCCCAGGCCATAAGATTCCCAGTGACAGGGACCTGGTTTCCCAAGGAGCCTCA
CAGCTCACATCATTGGAGATAAACTTTTCAACCAGCATTAAGAATGCCTGCCAAAAATATCAAGCCCAA
AGTATGTTTTTGTGTGACCCACGTGAACATTTGGTGATTAAACAGAAAGATGGGACTGTAGAAATGCGG
GGGAAAGACAAGAAGCAGCAGCCCAGGAAGGCCCACAGGGCCCACAGAGGCAGGCTCATAGCCAGCAAG
CAAAAACAAATCCCAATCCAGACCTGTAAATTCAATTCCAAATATCAGAGTCCTCAGCCTGCAATTTCT
GTCCCTCAAAGCCCCTTCCTGGGGCAGCAGGAACCCCGTGCTCAGCCTCTGCATCTGCAGTGTCCCAGG
AGCCCCAGAAAACCAGTCTGCCCTATTCTGGGAGGAACAGTCATGCCCAATAAGACAGTAAGGGCAGTG
CAGGGAAGACTCTTGCAGCTCAGCAGGTGCTCTTCCCAAGACAACTGGCTACTTTCCAGCAGTTCCCAG
GGGGACCACCAGATGAGCTGGTTCAGTGACCTCAATCTCGGATGTTCAGAGACCCCTCTATGCAAGGAG
GCAGGAAAGAATTTGCTCTATGACCTGGGTTTTGATAGCAGTGATGATGATGGCTTCTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_178173
Insert Size 1785 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178173.3
RefSeq Size 2994 bp
RefSeq ORF 1785 bp
Locus ID 339834
UniProt ID Q8IYA8
Cytogenetics 3p21.31
MW 66.3 kDa
Summary Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_178173" in other vectors (5)
SKU Description Size Price
RC206914 CCDC36 (Myc-DDK-tagged)-Human coiled-coil domain containing 36 (CCDC36), transcript variant 1 10 ug
$544.00
RC206914L3 Lenti ORF clone of Human coiled-coil domain containing 36 (CCDC36), transcript variant 1, Myc-DDK-tagged 10 ug
$844.00
RC206914L4 Lenti ORF clone of Human coiled-coil domain containing 36 (CCDC36), transcript variant 1, mGFP tagged 10 ug
$844.00
RG206914 CCDC36 (tGFP-tagged) - Human coiled-coil domain containing 36 (CCDC36), transcript variant 1 10 ug
$489.00 $744.00
SC126356 CCDC36 (untagged)-Human coiled-coil domain containing 36 (CCDC36), transcript variant 1 10 ug
$545.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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